The Glycomics Core provides services, instrumentation and expertise in glycomics to BIDMC research groups, affiliated and non-affiliated academic institutions and corporates.
Mass Spectrometer used for oligosaccharide structural analyses and MS-MS analysis.
Chromatography systems (3 systems) including autosamplers, fluorescence and UV detectors, and fraction collectors to isolate, purify and fractionate glycan compounds for structural analyses and microarray printing.
Non-contact printer used for high precision printing glycan or protein microarrays.
Microarray scanner used to quantify fluorescent signals for glycan microarray slides.
Mass spectrometer used for glycan profiling and structural analysis.
Consult with the Glycomics Core team to move your glycomics project(s) forward. The Glycomics Core can provide assistance with experimental and project design, grant proposal, research and data interpretation.
- Divers reagents (antibodies, cell lines, etc…) are made available to the research community only for the cost of shipping and handling. Reagents are shipped overnight on dry ice or ice packs.
- Analysis of potential glycan-binding proteins (GBPs) and antibodies, inactivated bacteria or virus on various glycan arrays including CFG glycan array, microbial glycan array, NCFG-derived defined or shotgun arrays
- Printing of custom microarrays on glass slides using natural occurring glycans, commercially available or glycopeptides/glycoproteins.
Glycan microarray analysis is currently processed following NCFG and CFG request procedures. More information can be found on their respective website. It will be requested from investigator to briefly describe and explain the nature of their projects.
Microarray printing service is also available, whether you need to print glycans or peptides/proteins.
N-glycan and O-glycan profiling of glycoproteins – purified glycoproteins, serum/plasma, cells or tissues by MALDI-TOF (Matrix-Assisted Laser desorption/Ionization) and other related technologies.
Glycolipids analysis – analysis of the glycan part of glycolipids isolated from cells or tissues by MALDI-TOF.
Sialic acid linkage analysis - discriminate between α2,3-linked sialic acid and α2,6-linked sialic acid by MALDI-TOF.
Mucin glycan analysis – analysis of mucin O-glycans.
Although the quantity of material provided to the core facility can be discussed on a case by case basis we propose the following guideline:
- Purified glycoprotein: 50 µg
- Serum: 20 µl
- Cells: 5.10^6 cells
- Tissue: 50 mg
Several enzymes are currently available, including β4GalT, Fut6, ST3Gal4 and ST6Gal1.