Viral Core’s goal is to provide scientists with access to cutting-edge viral vector technologies in an effective, high-quality and cost-efficient fashion. Viral Core also provides consultation to investigators in selecting, designing and using viral vectors.
We purify AAVs using Iodixanol gradients and ultracentrifugation. We could prep AAV vectors into serotype 1, 2, 5, 6, 7, 8, 9, rh10 and DJ. For a regular AAV prep, we need at least 150ug maxi-prep plasmid DNA. Typical virus yield is between 250-400ul. Genomic titer is quantified using Q-PCR. Purified AAV is good for in vivo applications.
Our lenti virus is pseudotyped with vsvg. We concentrate lenti virus by ultracentrifugation and quantify its genomic titer by Q-PCR.
Please provide at least 100ug plasmid DNA for a lenti prep. Typical virus yield is between 150-200ul.
We provide pre-made AAVs with different serotypes for AAV-GFP, AAV-Cre and AAV-ChR2. For detailed information of in stock pre-made AAVs, please email firstname.lastname@example.org.