The core is equipped with the following instruments: BIO-RAD QX200 Droplet Digital PCR System, Illumina MiSeq Next Generation Sequencer, Arcturus XT Laser Capture Microdissection System, Agilent 4200 TapeStation Bioanalyzer, Covaris E220 Ultra-Sonicator, ABI 7000 and 7500 Fast Real-Time qPCR systems.
On an hourly fee basis we provide: training on equipment usage, consultations, core setup, data analysis, troubleshooting, primer design. On per sample or per run fee basis we provide: ddPCR runs, qRT-PCR runs, MiSeq NGS runs, quality and quantity DNA and RNA analysis, and Covaris ultrasonicator sample shearing.
For details contact Dr. Petkova.
This quality and quantity control bioanalyzer is a powerful tool for accurate measurement of your DNA or RNA sample and will get you ready for your next step of application. The DNA ScreenTape will provide you with your sample’s QC needed in NGS, cDNA libraries preparation, genotyping, QC of qPCR products, PCR fragment analysis, mycoplasma detection. The RNA ScreenTape analysis will facilitate the preciseness of your gene expression profiling, qRT-PCR, NGS or microarray experiments.
The Adaptive Focused Acoustics (AFA) technology employs a very high frequency ultrasound waves to be focused into a discrete zone in the sample vessel. This efficient delivery requires minimal energy avoiding the adverse effect on the sample. The focused ultrasonicator provides exquisite control and tuning of the power, making the application of the instrument versatile: from gentle, low-power to high-power applications such as chromatin shearing, DNA fragmentation, liposome and nanosuspension formation.
"Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths."
After being trained on the proper usage of the LCM microscope, you will be free to reserve time on the LCM through an on-line calendar and work with the equipment. Come over and enjoy this powerful, state-of-the art instrument.
You bring your libraries for sequencing, we run them and provide you with the .fastq for further analysis.
Real-Time PCR will always remain the technology you will need for the final touch of your gene expression studies. Our core is equipped with all an investigator needs for primer design, experimental run and data processing. If you have been trained to operate a Real-Time PCR machine you can perform the experiment yourselves. Or, we can do it for you. You can purchase from us all the necessary reagents, endogenous controls and consumables. Or, you can use your own.