The Microscopy Core of the Program in Membrane Biology (PMB) at MGH is equipped and staffed to provide a wide range of services to investigators from MGH and the Boston area scientific community in the area of light and electron microscopy. The Core is housed on the 8th floor of the Simches Research Center at MGH, and is directed by Dr. Dennis Brown, Ph.D. The Core will provide services ranging from complete performance of the technical procedures required, to full training of personnel. Among the techniques available are laser scanning confocal microscopy, spinning disk confocal microscopy, tissue fixation, sectioning, immunostaining and conventional immunofluorescence microscopy, image analysis, and all aspects of electron microscopy including immunogold staining. The Core facility provides a unique and important resource for members of the scientific community as they approach a variety of research problems that call for these advanced microscopy techniques.
Thus, the Microscopy Core has a variety of menus to fit the microscopy and immunocytochemistry needs of most PIs. Interaction usually involves a preliminary discussion with the Core director and/or other Core staff who will be involved in the work. Intellectual and practical input are then tailored to fit individual requirements.
JEOL 1011 electron microscope with fully digital image capture and processing.
Primarily designed for the demanding needs of cryosectioning in biomedical, neuro-anatomical and pharmaceutical research
The Leica CM3050 S cryostat features superior user comfort with excellent safety standards for practically all types of cryosectioning applications.
It is the instrument of choice for all research applications and for advanced clinical cryosectioning needs.
Particularly when working with delicate specimens – for example brain samples in neuroscience – the precise specimen orientation and the specimen feed system guarantees reproducible, thin, serial sections of maximum quality.
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins.
The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.
For ultrathin frozen sectioning.
For paraffin sectioning.
The Ultramicrotome Leica EM UC7 provides easy preparation of semi- and ultrathin sections as well as perfect, smooth surfaces of biological and industrial samples for TEM, SEM, AFM and LM examination.
Combining ergonomic design and innovative technology the Ultramicrotome Leica EM UC7 sets new standards in Ultramicrotomy. It offers a range of outstanding features and benefits of use for the ultramicrotomist, whether highly skilled or absolute beginners.
MMI CellCut laser microdissection system
Nikon 80i upright epifluorescence microscope with Hamamatsu Orca CCD camera. Includes complete image analysis software (IP Lab).
Features fully digital image capture and processing.
Features Hamamatsu Orca CCD camera, Nikon Elements software and motorized XYZ stage.
The Nikon Eclipse 90i offers extensive motorized functions of its main body and with its accessories. On the main body, the 90i features a motorized fine-coarse control with a maximum speed of 5mm per second and an encoder-incorporated Z-axis provides resolution to 0.05 um and reproducibility to 0.1 um. In addition, a convenient control switch at the front of the microscope manages motorized light distribution, nosepiece rotation, fine or coarse focusing with quick drop function, handles the Epi fluorescence shutter, light intensity control, filter cube rotation and aperture along with the field diaphragms.
The 90i's motorized accessories include an XY Stage, Universal Condenser and DIC nosepiece that allows fast selection between high-contrast and high-resolution imaging capabilities. Nikon's 'fly-eye' lens array provides an even intensity that completely fills the objective back aperture and satisfies the strict demands of high-resolution digital imaging. Construction of multidimensional, ultra-high resolution images is made possible with Nikon's new violet corrected (VC) Plan APO optics and C1 Digital Eclipse Laser System. The VC Plan APO optics expand the spectral correction range into the violet lines, offer improved aberration correction and provide crisper and cleaner images across a broad range of observation conditions.
Nikon TE300 inverted microscope with wide-field optics and Hamamatsu Orca CCD camera. For fluorescence and ratio imaging, with Improvision OpenLab software.
Nikon Total Internal Reflection Fluorescence (TIRF) microscope system
Olympus double-headed light microscope. For section examination.
Two stand alone PC-based computer platforms with Nikon Elements, Zeiss Zen and Perkin Elmer Volocity image analysis software
Dr. Dennis Brown and the advisory committee will continually explore new techniques to be offered as part of the core service.
Dr. Dennis Brown and the Core technologists will be available for consultation in the use of the different microscopy techniques, and to provide technical advice, such as the different fixation techniques that enable antibodies to detect their antigens.
The core technicians will also provide training to high frequency users who wish to invest the substantial time necessary for learning to use the equipment proficiently (with the exception of the electron microscope).
The core will perform conventional immunofluorescence microscopy, confocal microscopy, live-cell imaging, conventional electron microscopy and EM immunogold labeling, tissue sectioning, tissue fixation, and computer based image analysis and quantification. In some cases, investigators will provide their own stained tissues/cell for imaging, while in others the Core will perform all services from fixation and sectioning, to staining and imaging.
"Scanalytics IPLab for Windows and Macintosh is image acquisition, processing, measurement, and analysis software. IPLab integrates image acquisition, external device control, an easy scripting utility, and advanced measurement and processing tools. You can easily expand IPLab's functionality at any time with extensions such as Ratio Plus, MultiProbe, and Motion Control."
"NIS-Elements AR provides a total imaging solution covering image capture, archiving, and analysis. It was developed by Nikon for the most advanced levels of research—giving the user complete control of all microscope and camera functions."
"High Performance 3D-4D imaging software for a better insight to your science."
Includes Zen Black and Zen Blue