The Immunology Division Flow Cytometry Facility provides flow cytometric analysis and cell sorting services to investigators in the Harvard Medical School, The Harvard Stem Cell Institute, and in the Harvard affiliated hospitals and institutions. The facility currently has an LSRII and a FACSCalibur self-run acquisition and analysis for staff trained researchers, and a FACSAria IIu and a MoFlo Astrios EQ for cell sorting (performed by an expert technician).
FACSAria for high-speed cell sorting, equipped with 4 lasers (355 (UV), 405, 488, and 638 nm) and 16 detectors for up to 14-color discrimination, and 4-way sorting.
FACSCalibur cytometer (2 Laser/4 color) available for assisted as well as unassisted flow cytometric acquisition and analysis.
The Astrios is the newest generation MoFlo on the market, offering many exciting new possibilities: 18 parameter 6 way sorting, reliable 384 well single cell plate sorting, a BL2 biosafety hood, and sort speeds of up to 70,000 cells/sec.
3-laser (405, 488 and 633 nm) 9-detector with a high throughput sampler for multiparameter flow analysis.
"BD FACSDiva™ software is a collection of rich tools for flow cytometer and application setup, data acquisition, and analysis that help streamline flow cytometry workflow for today's busy laboratory. BD FACSDiva software offers built-in features for consistent and maintainable daily cytometer setup that supports reproducible data acquisition and reliable results."