The Enhanced Neuroimaging Core offers comprehensive training, consultation and support throughout every phase of an imaging experiment. Image acquisition is available on several systems offering confocal, multi-photon, epi-fluorescence, brightfield, DIC, phase contrast, and 6D live cell imaging. Image analysis is supported through several high-end analysis platforms. Please contact Lai Ding, the optical imaging manager, or Daniel Tom, the optical imaging specialist to discuss your imaging needs.
The bidirectional triple light sheet technology of the UltraMicroscope II generates 6 focused light sheets to excite samples from the side while the fluorescence light is detected by a sCMOS camera perpendicular to the illumination plane. Moving the sample through the light sheet generates a 3D image stack. Selective excitation of the focal plane reduces bleaching and photo toxicity significantly. The open setup allows the analysis of cleared samples in any clearing solution or in aqueous media. The dynamic horizontal light sheet focus guarantees excellent Z-resolution covering the entire field of view.
The Leica SP8 X is a high sensitivity confocal microscope system that offers complete flexibility for any fluorescence experiment.
List of features include: white light laser, spectrophotometric detector, humidified stage top incubator, resonant scanner, large field of view, automated stage for confocal montage, motorized DIC, laser based autofocus, galvo-z, and HyD sentisitive detectors.
Provides all the features needed for routine confocal techniques; offering excellent image quality.
Spectrophotometric detector - User definable emission bandwidths
Automated stage for confocal montage images.
Motorized DIC - Automatically inserts the correct DIC Prism, Polarizer, and Analyzer. The bias of the prism can be stored and applied automatically.
Precise galvo-Z for quick Z-stack imaging.
• Two depletion lasers (592 and 660 nm) for super-resolution imaging of green and red dyes
• Leica DMI 6000 inverted microscope
• Pulsed white-light laser for tunable excitation and gated detection
• High-speed resonant scanner for live-cell imaging
• 3 HyD and 2 PMT detectors
• CW laser lines for conventional confocal imaging: 405, 458, 488, 514 nm
• Tokai Hit stagetop incubator
• Motorized xy stage
• LAS AF software for acquisition; Huygens Professional software for deconvolution
• Achievable resolution: < 40 nm lateral, and < 150 nm axial
"Microscope has three visible wavelength lasers and META spectral emission detectors which can precisely and effectively separate strongly overlapping fluorescence emission spectra.
The Two-Photon system features the Coherent Chameleon™ Multi-photon Excitation (MPE) laser tunable from 720nm – 930nm with 4 NLOs (External detectors). Designed specifically for MPE microscopy, the Chameleon™ provides widely tunable femtosecond pulses with more than 1W of average power. The Chameleon can excite all the fluorophores commonly used in MPE. The longer wavelengths of the Chameleon reduce cell photo bleaching, and allow biologists to image deeper into tissue due to less light scatter, with less tissue damage."
"Although the facility primarily supports NeuroDiscovery investigators studying neurodegenerative disease, we welcome inquires from other users.
All users must complete training before access to the facility is granted.
Experienced users are provided access out of the regular hours with consent from the Facility Manager. Only those users with sufficient training on the microscopes can gain access to the facility during off-peak hours."
Our core provides image analysis service on custom plugin/macro-script for ImageJ; Journal for Metamorph and custom image analysis protocols. Analysis service includes image filtering, cell counting, image segmentation and measurement, batch data process, particle analysis. The service charge is based on hourly rate same as the microscopy usage. For projects with images taken in our core, this service is free.
"Imaris is Bitplane’s core scientific software module that delivers all the necessary functionality for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size. Conveniently load, process and visualize data and images acquired from almost any confocal and wide field microscope to gain new and groundbreaking insight from your image data."
"ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.
It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.
It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."
"MetaMorph® Microscopy Automation & Image Analysis Software is the industry standard for automated microscope acquisition, device control, and image analysis, bringing microscopists greater understanding of cell morphology, function, and behavior for over 25 years."