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Cellular Imaging Core (BCH)

Location: Children's Hospital, Division of Neuroscience, 300 Longwood Avenue, CLS13035, Boston, MA 02115

Summary:

The Imaging Core provides access to standard digital and laser scanning confocal microscopy, multiphoton microscopy, unbiased stereology, and automated confocal microscopy for large scale genome-wide screens. In addition, the Core has substantial capabilities in quantitative and three-dimensional volumetric image reconstruction.

Affiliations:

People:

Resources:

Instruments

  • 100X Nikon Plan Fluor ( Objective lens )

    Part of Nikon E800 microscope (oil) workstation; oil, 1.3NA.

  • 100X Zeiss plan-APOCHROMAT ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • 10X Nikon Plan Apo ( Objective lens )

    Part of Neurolucida and Stereo Investigator Imaging Station; dry, 0.45NA.

  • 10X Nikon Plan Fluor ( Objective lens )

    Part of Nikon 80i microscope (air) workstation; dry, 0.3NA.

  • 10X Nikon Plan Fluor ( Objective lens )

    Part of Nikon E800 microscope (oil) workstation; dry, 0.3NA.

  • 10X Zeiss plan-NEOFLUAR ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; dry, 0.3NA.

  • 1X Nikon Plan UW ( Objective lens )

    Part of Nikon E800 microscope (oil) workstation; dry, 0.04NA.

  • 20X Nikon Plan Apo ( Objective lens )

    Part of Neurolucida and Stereo Investigator Imaging Station; dry, 0.75NA.

  • 20X Nikon Plan Fluor ( Objective lens )

    Lab contains two of these. Part of Nikon E800 microscope (oil) workstation; oil, 0.75NA and Nikon 80i microscope (air) workstation; dry, 0.5NA.

  • 25X Zeiss plan-NEOFLUAR ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; multi-immersion, 0.75N.

  • 2X Nikon Plan Apo ( Objective lens )

    Lab contains two of these. Part of Neurolucida and Stereo Investigator Imaging Station; dry, 0.1NA and Nikon 80i microscope (air) workstation; dry, 0.1NA.

  • 40X Nikon Plan Apo ( Objective lens )

    Lab contains two of these. Part of Neurolucida and Stereo Investigator Imaging Station; dry, 0.95NA and Nikon 80i microscope (air) workstation; dry, 0.95NA.

  • 40X Nikon Plan Fluor ( Objective lens )

    Part of Nikon E800 microscope (oil) workstation; oil, 0.3NA.

  • 40X Zeiss plan-NEOFLUAR ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • 40X Zeiss plan-NEOFLUAR ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; water, 0.6NA.

  • 458, 488 and 514 nm: Argon-ion laser, 30 mW ( Gas laser )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • 4X Nikon Plan Apo ( Objective lens )

    Part of Nikon 80i microscope (air) workstation; dry, 0.2NA.

  • 543 nm: Helium-Neon laser, 1mW ( Helium neon ion laser )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • 5X Zeiss plan-APOCHROMAT ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation;dry, 0.16NA.

  • 60X Nikon Plan Apo ( Objective lens )

    Lab contains two of these. Part of Nikon E800 microscope (oil) workstation; oil, 1.4NA and Nikon 80i microscope (air) workstation; dry, 0.95NA.

  • 633 nm: Helium-Neon laser, 15mW ( Helium neon ion laser )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • 63X Zeiss plan-APOCHROMAT ( Objective lens )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.4NA.

  • 720-930 nm: tunable Chameleon IR laser, 1,870 mW max (2-photon excitation) ( Argon ion laser )

    Part of Zeiss Axioplan 2 microscope (upright) workstation; oil, 1.3NA.

  • Camera lucida ( Microscope digital camera )

    Attached to Zeiss Axioskop microscope.

  • CY5 fluorescence filter cube ( Optical filter )

    Lab contains three copies of this instrument. Part of the Nikon E800 microscope (oil) workstation, Nikon 80i microscope (air) workstation, and Neurolucida and Stereo Investigator Imaging Station.

  • DAPI fluorescence filter cube ( Optical filter )

    Lab contains two copies of this instrument. Part of the Nikon 80i microscope (air) workstation and Neurolucida and Stereo Investigator Imaging Station.

  • Digital camera ( Digital camera )

    Part of the Neurolucida and Stereo Investigator Imaging Station.

  • Fiber optics illuminator ( Light source )

    Attached to SZ654 microscope

  • FITC fluorescence filter cube ( Optical filter )

    Lab contains three copies of this instrument. Part of the Nikon 80i microscope (air) workstation, Nikon E800 microscope (oil) workstation, and Neurolucida and Stereo Investigator Imaging Station.

  • Hamamatsu ORCA-R2 CCD camera ( Charge-coupled device camera )

    Part of the Nikon 80i microscope (air) workstation.

  • Leica TCS SP8 STED One ( Laser scanning confocal microscope )

    The Leica SP8 is a high sensitivity point scanning confocal that provides very-high-quality images with fine detail and more contrast than conventional microscopy. In addition, the imaging technique allows for reconstruction of virtual 3-dimensional (3-D) images (static or time lapse) of the tissue when multiple (optical) sections are combined. Having an encoded motorized stage allows reproducible positioning of sample and automated mosaic tiling for imaging large areas without sacrificing spatial resolution. Besides, its resonant scanner for low photo-toxicity makes it ideal for live cell imaging.

  • Leica TCS SP8 STED One workstation ( Laser scanning confocal microscope )

    The Leica SP8 is a high sensitivity point scanning confocal that provides very-high-quality images with fine detail and more contrast than conventional microscopy. In addition, the imaging technique allows for reconstruction of virtual 3-dimensional (3-D) images (static or time lapse) of the tissue when multiple (optical) sections are combined. Having an encoded motorized stage allows reproducible positioning of sample and automated mosaic tiling for imaging large areas without sacrificing spatial resolution. Besides, its resonant scanner for low photo-toxicity makes it ideal for live cell imaging.

  • Motorized XY stage and Z-axis focus ( Motorized stage )

    Part of the Neurolucida and Stereo Investigator Imaging Station.

  • Neurolucida and Stereo Investigator Imaging Station ( Fluorescence microscope )

    Has parts:
    Nikon 80i upright microscope,
    Motorized XY stage and Z-axis focus,
    2X Nikon Plan Apo,
    10X Nikon Plan Apo,
    20X Nikon Plan Apo,
    40X Nikon Plan Apo,
    Fluorescence filters cubes - DAPI,
    Fluorescence filters cubes - FITC,
    Fluorescence filters cubes - Rhodamin,
    Fluorescence filters cubes - Cy5,
    camera.

  • Nikon 80i microscope (air) workstation ( Fluorescence microscope )

    Has parts:
    Nikon 80i microscope (air) microscope,
    2X Nikon Plan Apo,
    4X Nikon Plan Apo,
    10X Nikon Plan Fluor,
    20X Nikon Plan Fluor,
    40X Nikon Plan Apo,
    60X Nikon Plan Apo
    Fluorescence filters cubes DAPI,
    Fluorescence filters cubes FITC,
    Fluorescence filters cubes Rhodamin,
    Fluorescence filters cubes Cy5,
    Hamamatsu ORCA-R2 CCD camera.

  • Nikon 80i microscope (air) microscope ( Microscope )

    Part of Nikon 80i microscope (air) workstation.

  • Nikon 80i upright microscope ( Upright microscope )

    Part of the Neurolucida and Stereo Investigator Imaging Station. The Neurolucida 3D analysis system is used for high resolution neuron tracing, brain mapping, serial section reconstruction, and neuroanatomical analysis. It can also perform cell counting in 3D space, volumetric analysis, and interactive and automatic particle analysis.

  • Nikon E800 microscope (oil) ( Microscope )

    Part of the Nikon E800 microscope (oil) workstation.

  • Nikon E800 microscope (oil) workstation ( Fluorescence microscope )

    Has parts:
    Nikon E800 microscope (oil) microscope,
    1X Nikon Plan UW,
    10X Nikon Plan Fluor,
    20X Nikon Plan Fluor,
    40X Nikon Plan Fluor,
    60X Nikon Plan Apo,
    100X Nikon Plan Fluor,
    Fluorescence filters cubes - UV,
    Fluorescence filters cubes - FITC,
    Fluorescence filters cubes - TRITC,
    Fluorescence filters cubes - Tx RED,
    Fluorescence filters cubes - CY5,
    Spot RT cooled CCD camera.

  • Photo-camera ( Microscope digital camera )

    Attached to Zeiss Axioskop microscope.

  • Remote Confocal Image Processing PC Workstation ( Computer workstation )

    Workstation 1

  • Rhodamin fluorescence filter cube ( Optical filter )

    Lab contains two copies of this instrument. Part of the Nikon 80i microscope (air) workstation and Neurolucida and Stereo Investigator Imaging Station.

  • Spot RT cooled CCD camera ( Charge-coupled device camera )

    Part of the Nikon E800 microscope (oil) workstation; includes both color and black & white imaging.

  • TRITC fluorescence filter cube ( Optical filter )

    Part of Nikon E800 microscope (oil) workstation.

  • Tx Red fluorescence filter cube ( Optical filter )

    Part of Nikon E800 microscope (oil) workstation.

  • UV fluorescence filter cube ( Optical filter )

    Part of Nikon E800 microscope (oil) workstation.

  • Zeiss Axioplan 2 microscope ( Upright microscope )

    Part of the Zeiss Axioplan 2 microscope (upright) workstation.

  • Zeiss Axioplan 2 microscope workstation ( Two-photon confocal microscope )

    Has parts:
    Zeiss Axioplan 2 microscope,
    5X Zeiss plan-APOCHROMAT,
    10X Zeiss plan-NEOFLUAR,
    25X Zeiss plan-NEOFLUAR,
    40X Zeiss plan-NEOFLUAR,
    40X Zeiss plan-NEOFLUAR,
    63X Zeiss plan-APOCHROMAT,
    100X Zeiss plan-APOCHROMAT,
    458, 488 and 514 nm: Argon-ion laser, 30 mW,
    543 nm: Helium-Neon laser, 1mW,
    633 nm: Helium-Neon laser, 15mW,
    720-930 nm: tunable Chameleon IR laser, 1,870 mW max (2-photon excitation).

  • Zeiss Axioskop microscope ( Microscope )

  • Zeiss Axioskop microscope workstation ( Digital microscope )

    Has parts: Zeiss Axioskop microscope, camera lucida, photo-camera.

Software

  • ImageJ ( Software )

    Available on two workstations; free software package specifically tailored for fluorescence microscopy. "ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.

    It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.

    It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."

  • Imaris 7 workstation ( Algorithmic software suite )

  • Zeiss LSM Image Browser ( Software )

    Available for workstation 2. Image processing software.


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Last updated: 2019-09-10T15:21:19.467-04:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016