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Biochemical assays

eagle-i ID

http://harvard.eagle-i.net/i/00000166-ac7e-5d59-60f6-3e5480000000

Resource Type

  1. Support service

Properties

  1. Resource Description
    • Measurement of hormones and metabolites in plasma/serum of mice, rat or human subjects (Elisa, RIA, WB). Hormones: Insulin, leptin, adiponectin, resistin, RBP4, TTR. Metabolites: Glucose, free fatty acids, triglycerides, cholesterol. For measurement of other hormones or metabolites, contact Dr Peroni • Insulin signaling: PI-3 kinase, Akt, GSK3, p70s6 kinase, MAP kinase, glycogen synthase activation; phosphorylation of insulin receptor and IRSs. • Leptin signaling: phosphorylatiom of Jaks, stats, AMP kinase, MAP kinase, acetyl-coA carboxylase, p70S6 kinase phosphorylation. • Enzyme activity assays: AMP Kinase immune complex assay, Glucose 6-Phosphatase • Tissue glycogen or lipid content.
  2. Contact
    Wellenstein, Kerry
  3. Service Provided by
    BADERC Metabolic Physiology Core (HMS)
 
RDFRDF
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016