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Cryopreservation, IVF, & Strain Rederivation

eagle-i ID

http://harvard.eagle-i.net/i/0000015a-d7ba-92b8-5df2-764380000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    • Sperm Cryopreservation - including quality control (QC) to 2-cell test • Recovery live pups for testing, following sperm cryopreservation and QC 2-cell test • IVF recovery of mouse lines from cryopreserved sperm (one litter of pups provided) • IVF with fresh mouse sperm for rapid expansion to produce dozens or hundreds of age-matched mouse colonies • Mouse embryo Cryopreservation • Resuscitate of cryopreserved mouse embryos to generate mouse colony • Rederivation of mouse strains by embryo transfer to generate pathogen free mouse lines
  3. Service Fee URL
    http://gmf.fas.harvard.edu/pages/cryopreservation
  4. Contact
    Wu, Lin, Ph.D.
  5. Access Restriction(s)
    Service fees for external, non-Harvard investigators are given upon request.
  6. Service Provided by
    Genome Modification Facility, Harvard University
  7. Related Technique
    Cryoembedding
  8. Related Technique
    In vitro fertilization
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016