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Microinjection of CRISPR Reagents, ES or iPS Cells, DNA, & TALEN

eagle-i ID

http://harvard.eagle-i.net/i/0000015a-d76c-6523-5df2-764380000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    • Microinjection of CRISPR reagents or TALENs into mouse zygotes for gene knockout projects • Microinjection of CRSIPR reagents or TALENs into mouse zygotes for gene knockin projects • Microinjection of ES clones (targeted C57BL/6, 129, JM8A, or hybrid ES Cells) into embryos of C57BL/6, B6-albino, hybrid, or CD1 for generating gene targeted mice • Microinjection of iPS clones into embryos of C57BL/6, B6-albino, hybrid, or CD1 • Microinjection of plasmid DNA into pronuclei of embryos: hybrid mouse strain, C57BL/6, FVB, Balb/C, NOD, or mutant strains for generating transgenic mice • Microinjection of BAC or YAC DNA into pronuclei of embryos: hybrid mouse strain, C57BL/6, or others
  3. Service Fee URL
    http://gmf.fas.harvard.edu/pages/blastocyst-injection
  4. Contact
    Wu, Lin, Ph.D.
  5. Access Restriction(s)
    Service fees for external, non-Harvard investigators are given upon request.
  6. Service Provided by
    Genome Modification Facility, Harvard University
  7. Related Technique
    Transgenic mouse model generation
  8. Related Technique
    Microinjection
 
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Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016