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Custom made AAV prep

eagle-i ID

http://harvard.eagle-i.net/i/00000151-ac16-0604-6771-1ce080000000

Resource Type

  1. Material processing service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    We purify AAVs using Iodixanol gradients and ultracentrifugation. We could prep AAV vectors into serotype 1, 2, 5, 6, 7, 8, 9, rh10 and DJ. For a regular AAV prep, we need at least 150ug maxi-prep plasmid DNA. Typical virus yield is between 250-400ul. Genomic titer is quantified using Q-PCR. Purified AAV is good for in vivo applications.
  3. Service Fee URL
    http://www.childrenshospital.org/research-and-innovation/research/centers/fm-kirby-neurobiology-center/cores/viral-core
  4. Service Provided by
    Boston Children's Hospital Viral Core
  5. Related Technique
    Virus preparation
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016