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14F = yMK886
eagle-i ID
http://harvard.eagle-i.net/i/0000012d-c2d5-273a-4882-b08d80000000
Resource Type
Properties
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Resource Description
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MATa ADE2+ his3-GCN4RE mutated leu2- LYS2+ MET15+ trp1-ura3-DED1 w/GCN4RE.
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Resource Description
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PLUS insertion of two optimal. GCN4 binding sites in the DED1 promoter (XhoI) ATGAGTCATtcgaATGACTCAT.
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Resource Description
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as yMK869: wt GCN4RE of HIS3 (ATGACTCTT) changed to 'GTCGACGTC', effectively destroying the GCN4 binding site
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Related Publication or Documentation
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Gcn4 activator targets Gcn5 histone acetyltransferase to specific promoters independently of transcription
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Parental Strain Name
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yMK886
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Location
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Struhl Laboratory
