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YCplac22 5myc DOT1
eagle-i ID
http://harvard.eagle-i.net/i/0000012d-7518-c448-87dd-920c80000000
Resource Type
Properties
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Construct Insert
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DOT1
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Resource Description
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The DOT1 gene (including 600 bp upstream of ATG, and 250 bp downstream of the term codon) was cloned by PCR as a SphI-XmaI fragment into the corresponding sites of YCplac22.
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Construct Backbone
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YCplac22
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Related Publication or Documentation
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Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association
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Selectable Marker
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TRP1
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Location
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Struhl Laboratory
Harvard University
