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Ambion Human 1: Mimics

eagle-i ID

http://harvard.eagle-i.net/i/0000012c-a895-f9a5-02c0-2b6880000000

Resource Type

  1. miRNA library

Properties

  1. Library Source
    ICCB-Longwood Screening Facility (HMS)
  2. Resource Description
    384-well plate format. Concentration Information: 1uM in buffer. Control Information: Pre-miR miRNA Precursor Molecules-Neg.Control #1 (Ambion Cat#: AM17110); Human PLK1 siRNA SMARTPool (Dharmacon Cat#: M-003290-01); Pre-miR miRNA Precursor Molecules-Neg. Control #2 (Ambion Cat#: AM17111). This library is the Ambion Human Pre-miR miRNA Mimic Library and was purchased in the Summer of 2009. The design of this library was based on miRBase release 13.0. The stock concentration for the siRNA library plates designated for screening is 1uM. Higher concentration stocks are available upon request. All of the siRNAs are stored in 1x Dharmacon siRNA buffer (20mM KCl, 6mM HEPES-pH 7.5, and 0.2 mM MgCl2). Screeners using 96-well plates for their assay will be provided with the appropriate information to translate 384-well plate numbers to 96-well plate numbers for the purposes of labeling assay plates.
  3. Contact
    Shamu, Caroline, Ph.D.
  4. Manufacturer
    0000013d-bc68-88e5-ecc6-81a580000000
  5. Access Restriction(s)
    Please note that ICCB-L provides these reagents only within the context of a full screen or an approved medicinal chemistry project.
  6. Website(s)
    https://iccb.med.harvard.edu/ambion-human-1-mimics
  7. Library Count
    Number of siRNA wells: 851
  8. Source Organism or Virus
    Homo_sapiens
  9. Location
    ICCB-Longwood Screening Facility (HMS)
 
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Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016