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Intact molecular weight determination service

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    Intact proteins, oligonulceotides and peptides frequently need an intact mass determination, and MALDI-TOF is the preferred method to obtain this information due to the "soft" ionization technique and low charge states associated with this technique. Samples are applied to a target with an appropriate matrix and allowed to dry fully, concentrating of sample in a crystalized matrix spot. A UV laser imparts energy to the sample through the matrix, causing the sample to ionize (typically a a singly or doubly charged species) and the time it takes to travel along the flight tube is proportional to the mass of the sample molecule. Typically proteins and peptides between 0.5 and 200kDa, and oligonucleotides up to 10kDa can be observed at very high sensitivity. Sample concentration is key to good signal quality, and salts, detergents and other compounds in the sample buffer can reduce the ionization of the molecule significantly.
  3. Related Resource
  4. Access Restriction(s)
    Different fees apply for academic and corporate users.
  5. Service Provided by
    Mass Spectrometry and Proteomics Research Laboratory (FAS)
  6. Website(s)
  7. Related Technique
    Protein mass determination by mass spectrometry
Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016