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Complex protein mixture analysis service

eagle-i ID

http://harvard.eagle-i.net/i/0000012b-00c0-b2e4-db6e-7a3f80000004

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    Complex mixtures of proteins are identified by a number of single- and multi-dimensional approaches. For example, GeLC, in which an entire lane of an SDS-PAGE gel is excised into sections, affords the user a two dimensional separation of the protein mixture based on protein intact molecular weight (SDS-PAGE) and then individual peptide hydrophobicity by reversed phase chromatography (RPLC). A similar method known as MUDPit (Multidimensional Protein Identification Technology) starts with a solution digestion of the sample, then two dimensional chromatography by strong cation exchange chromatography (SCX) followed by reversed phase chromatography (RPLC).
  3. Related Resource
    0000012b-00c0-2faa-db6e-7a3f80000000
  4. Access Restriction(s)
    Different fees apply for academic and corporate users.
  5. Service Provided by
    Mass Spectrometry and Proteomics Research Laboratory (FAS)
  6. Website(s)
    http://proteomics.fas.harvard.edu/pages/services-1
  7. Related Technique
    Protein electrophoresis
  8. Related Technique
    Protein identification
 
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Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016