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Cell sorting protocol

http://harvard.eagle-i.net/i/0000012a-438d-97d4-3e3e-48fb80000000

Resource Description

Assays run and analyzed by the facility include: Fluorescent cell labeling, non-fluorescent cell labeling, Endocytosis, Phagocytosis, Live-dead cell discrimination, Proliferation/DNA content/cell cycle analysis, Mitochondrial staining, Apoptosis, Antibody/antigen binding, non-invasive cell growth analysis, Cell isolation, and Intracellular immunophenotyping by cell permeabilization.
Uses

Becton Dickinson FACScan analyzer
Uses

Beckman Coulter FC 500 flow cytometer
Uses

MoFlo Astrios cell sorter
Uses

Cellular assay
Uses

Fluorescence activated cell sorting (FACS)
Used by

Flow Cytometry Core (BIDMC)

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