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RIP-tTA/N Tg

eagle-i ID

http://harvard.eagle-i.net/i/0000012a-25bf-7988-f5ed-943080000013

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    Development: The tetracycline regulatable transactivator (tTA, (Gossen M, Bujard H,1992; Gossen M, Bujard ,1995) is placed under the control of the rat insulin promoter (Ins2or RIP), and is expressed in the pancreatic beta cells. The RIP-tTA transgenic construct was injected into (C57BL/6J x SJL)F1 oocytes. The resulting line was backcrossed onto the NOD background for 22 generations. This strain provides a Tet-Off tool that permits the inducible expression of genes in the pancreatic beta cells during various stages of diabetes development. Mice hemizygous for this transgenic insert are viable, fertile, normal in size, display normal NOD diabetes onset, but do not display any other gross physical or behavioral abnormalities. When mated to a second transgenic strain carrying the target gene regulated by the tetO responsive elements, expression of the target gene is turned off by the tetracycline analog, doxycycline (dox). Dox can be administered orally in the food or water.
  2. Related Disease
    type 1 diabetes mellitus
  3. Related Publication or Documentation
    Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
  4. Related Publication or Documentation
    Transcriptional activation by tetracyclines in mammalian cells.
  5. Parental Strain Name
    C57BL/6J x SJL
  6. Genetic Alteration(s)
    Tetracycline controlled transactivator insertion
  7. Genetic Alteration(s)
    Rat Insulin promoter
  8. Clinical or Environmental Source
    Controlled facility at Jackson labs
  9. Location
    Genetically Modified NOD Mouse Core Facility (HMS)
 
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