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Ealpha16/N Tg

eagle-i ID

http://harvard.eagle-i.net/i/0000012a-25bf-7988-f5ed-943080000009

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    A cloned Ek alpha MHC Class II gene was introduced by microinjection into (C57BL/6 x SJL) F1 mouse oocytes. These strains, as NOD mice, are generally deficient in the MHC II E complex because of a large deletion in the promoter of he Ealpha gene. The transgene thus restore proper expression of the E alpha E beta complex, and immune reactivities linked to it (Le Meur et al, 1985). The line is currently at the >99th backcross generation in NOD/LtJ (Bohme J et al, 1990). Transgenic mice are viable and fertile. Ealpha 16 abundance in the spleen of all transgenic mice is equivalent to that in BALB/c and B10-BR controls. E alpha 16 expresses the I-E complex on all cells that normally display class II molecules. On the NOD genetic background, the transgene E alpha 16 confers almost complete protection from insulitis by preventing lymphocyte infiltration into the pancreatic islets of 10 week old mice.
  2. Related Disease
    type 1 diabetes mellitus
  3. Related Publication or Documentation
    Correcting an immune-response deficiency by creating E alpha gene transgenic mice.
  4. Related Publication or Documentation
    MHC-linked protection from diabetes dissociated from clonal deletion of T cells.
  5. Parental Strain Name
    NOD
  6. Genetic Alteration(s)
    E Alpha 16 insertion
  7. Clinical or Environmental Source
    Controlled facility at Jackson labs
  8. Location
    Genetically Modified NOD Mouse Core Facility (HMS)
 
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