eagle-i Harvard UniversityHarvard University
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Ealpha16/N Tg

eagle-i ID


Resource Type

  1. Mus musculus


  1. Resource Description
    A cloned Ek alpha MHC Class II gene was introduced by microinjection into (C57BL/6 x SJL) F1 mouse oocytes. These strains, as NOD mice, are generally deficient in the MHC II E complex because of a large deletion in the promoter of he Ealpha gene. The transgene thus restore proper expression of the E alpha E beta complex, and immune reactivities linked to it (Le Meur et al, 1985). The line is currently at the >99th backcross generation in NOD/LtJ (Bohme J et al, 1990). Transgenic mice are viable and fertile. Ealpha 16 abundance in the spleen of all transgenic mice is equivalent to that in BALB/c and B10-BR controls. E alpha 16 expresses the I-E complex on all cells that normally display class II molecules. On the NOD genetic background, the transgene E alpha 16 confers almost complete protection from insulitis by preventing lymphocyte infiltration into the pancreatic islets of 10 week old mice.
  2. Related Disease
    type 1 diabetes mellitus
  3. Related Publication or Documentation
    Correcting an immune-response deficiency by creating E alpha gene transgenic mice.
  4. Related Publication or Documentation
    MHC-linked protection from diabetes dissociated from clonal deletion of T cells.
  5. Parental Strain Name
  6. Genetic Alteration(s)
    E Alpha 16 insertion
  7. Clinical or Environmental Source
    Controlled facility at Jackson labs
  8. Location
    Genetically Modified NOD Mouse Core Facility (HMS)
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016