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RAG-1[0]/N

eagle-i ID

http://harvard.eagle-i.net/i/0000012a-25bf-7988-f5ed-943080000000

Resource Type

  1. Mus musculus

Properties

  1. Resource Description
    Development: A neomycin resistance cassette was inserted in the RAG-1 gene, resulting in a 1356 bp deletion in the 5' end of the coding sequence into 129-derived AB1 ES cell line. The C57BL/6J strain was generated by backcrossing mice carrying the Rag-10 mutation 10 times to C57BL/6J inbred mice. (Mombaerts P et al, 1992). Rag-1 deficient mice were transferred to the NOD/LtSz strain background. The line is currently at the 18th backcross to NOD. RAG-1 deficient mice are viable and fertile.They are unable to initiate V(D)J recombination in Ig and TCR genes and lack functional T and B lymphocytes. Although RAG-1 expression has been reported in the central nervous system of the mouse, no obvious neuroanatomical or behavioral abnormalities have been found in the RAG-1-deficient mice. On the NOD background, NOD.Rag10 mice are devoid of mature T or B cells. NOD/LtSz-Rag10 recipients of adoptively transferred spleen cells from diabetic NOD/Lt+/+ mice rapidly develop diabetes.
  2. Related Disease
    diabetes mellitus
  3. Related Publication or Documentation
    NOD/LtSz-Rag1null mice: an immunodeficient and radioresistant model for engraftment of human hematolymphoid cells, HIV infection, and adoptive transfer of NOD mouse diabetogenic T cells.
  4. Related Publication or Documentation
    Cutting edge: Cross-talk between cells of the innate immune system: NKT cells rapidly activate NK cells.
  5. Related Publication or Documentation
    RAG-1-deficient mice have no mature B and T lymphocytes.
  6. Parental Strain Name
    C57BL/6J
  7. Genetic Alteration(s)
    Recombination Activating Gene 1 deletion
  8. Clinical or Environmental Source
    Controlled facility at Jackson labs
  9. Phenotype Findings
    Unable to initiate V(D)J recombination in TCR and lack function T lymphocytes.
  10. Phenotype Findings
    Unable to initiate V(D)J recombination in Ig and lack functional B lymphocytes.
  11. Phenotype Findings
    NOD/LtSz-Rag10 recipients of adoptively transferred spleen cells from diabetic NOD/Lt+/+ mice rapidly develop diabetes.
  12. Location
    Genetically Modified NOD Mouse Core Facility (HMS)
 
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