Protein acetylation protocol

eagle-i ID

Resource Type

Properties

1. Resource Description

   "Protein lysine acetylation, referring to acetylation of the ɛ-amino group of a lysine residue, [is] an important post-translational modification for regulating protein functions in various organisms. Lysine acetylation is a rapidly reversible and precisely controlled covalent modification that serves as a simple on/off switch or participates in a codified manner with other post-translational modifications to regulate protein functions in different cellular and developmental processes. This unit describes and discusses methods used for in vitro and in vivo determination of lysine acetylation." Curr. Protoc. Protein Sci. 54:14.11.1-14.11.17.

2. Uses

   4800 MALDI-TOF/TOF mass spectrometer

3. Uses

   Experimental posttranslational modification

4. Used by

   Molecular Biology Core Facilities (DFCI)

5. Used by

   Mass Spectrometry Core (BIDMC)