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pTarT-4

http://harvard.eagle-i.net/i/0000012a-250b-df2e-5617-7942800001d2

Resource Description

Gene-trap with polyA trap (Pax2-splice doner) incorporated into the selectable marker. SA-NIRES-EGFP-pA rBA-DsRed-SD.
Resource Description

Lab was unable to grow high titer virus with this construct. Probably because the removal of the pA stop for the selectable marker (DsRed) put the 5' LTR in direct competition with the rat Beta-Actin constitutive promoter.
Additional Name

Gene Trap
Construct Backbone

pTarT
Selectable Marker

Chloramphenicol
Related Technique

Gene trapping
Location

Tabin Laboratory

Inferred Types from the eagle-i Ontology (What is an ontology?)

Provenance Metadata About This Resource Record