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pTarT-4
eagle-i ID
http://harvard.eagle-i.net/i/0000012a-250b-df2e-5617-7942800001d2
Resource Type
Properties
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Resource Description
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Gene-trap with polyA trap (Pax2-splice doner) incorporated into the selectable marker. SA-NIRES-EGFP-pA rBA-DsRed-SD.
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Resource Description
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Lab was unable to grow high titer virus with this construct. Probably because the removal of the pA stop for the selectable marker (DsRed) put the 5' LTR in direct competition with the rat Beta-Actin constitutive promoter.
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Additional Name
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Gene Trap
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Construct Backbone
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pTarT
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Selectable Marker
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Chloramphenicol
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Related Technique
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Gene trapping
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Location
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Tabin Laboratory
