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Molecular Pathology Core Lab (MPCL)

Location: Molecular Pathology Core Laboratory (MPCL), Dana-Farber Cancer Institute, 450 Brookline Avenue, Jimmy Fund Building JF-0215, Boston, MA 02215

Summary:

The Molecular Pathology Core Laboratory (MPCL) works to advance molecular pathology-based cancer research. The group addresses significant scientific and clinical research needs and has created a central hub where researchers can interact, collaborate, and utilize novel molecular pathology technologies to translate basic science discoveries into patient care applications for cancer. Our mission comprises comprehensive pathology annotation to encompass clinical, morphological, and molecular characteristics, genetic discovery in primary human tumors, with limited material and in real time, novel biomarker identification and pre-clinical trials, and transitioning assays to the clinical setting

Affiliations:

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    Resources:

    Instruments

    • LCM – Arcturus XT Laser Capture Microdissection ( Laser capture microdissection microscope )

      "The ArcturusXT™ microdissection system offers a unique combination of infrared (IR) laser–enabled laser capture microdissection (LCM) and ultraviolet (UV) laser cutting in one platform. The ArcturusXT™ LCM instrument and reagents, combined with our downstream analysis platforms like those for Sanger sequencing, next-generation sequencing, and quantitative PCR, provide a complete solution for microgenomics research."

    Services

    • General histology ( Material processing service )

      Paraffin tissue processing and embedding; frozen/FFPE tissue sectioning; H&E staining; cell pellet block preparation

    • Immunohistochemistry (IHC) ( Material analysis service )

      Chromogenic IHC (single/dual); multiplex fluorescence IHC (up to 6 markers/slide).

    • In Situ Hybridization (ISH) ( Material processing service )

    • Laser Capture Microdissection (LCM) ( Material processing service )

      LCM training and core operated services available, plus LCM instrument use.

    • Multispectral Imaging Analysis ( Material analysis service )

      Whole slide scanning – bright-field, fluorescence (10X, 20X, 40X); ROI image analysis – multispectral composite, segmentation, phenotyping, scoring TMA scanning and analysis.

    • TUNEL ( Material analysis service )

      Chromogenic/fluorescence


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    Last updated: 2019-04-05T14:11:23.515-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016