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Nutritional Biomarker Laboratory

Director: Furtado, Jeremy D., ScD

Location: 665 Huntington Ave, Bldg 2, Room 243, Boston, MA 02115

Summary:

The Nutritional Biomarker Laboratory at the Harvard T.H. Chan School of Public Health has more than 30 years of expertise in the analysis of antioxidants and fatty acids in blood, tissues, and foods backed by external quality monitoring programs administered by the National Institute of Standards and Technology (NIST), the American Oil Chemists’ Society (AOCS), and the Centers for Disease Control and Prevention (CDC). More recently we have added a blood lipid panel, 25-OH vitamin D EIA assay, tyrosol and hydroxytyrosol in urine and plasma, and various ELISA-based assays. We are skilled at the development of new assays that are amenable to our instruments and are happy to discuss projects beyond the scope of our standard panels.

Affiliations:

People:

Resources:

Instruments

  • Agilent 6890 GC-FID ( Flame ionization detector )

  • Agilent 6890 GC-MS ( Gas chromatography-mass spectrometry platform )

  • Biotek Synergy HT microplate reader ( Multimode microplate reader )

    "BioTek Instruments have developed the Synergy™ HT Multi-Mode Microplate Reader. This plate reader for fluorescence, absorbance and luminescence measurements was designed with an emphasis on superior performance in all detection methods. Unlike many multi-mode microplate readers, the Synergy HT utilizes a unique dual-optics design."

  • Hitachi LaChrom HPLC system ( High performance liquid chromatography instrument )

    "Hitachi high-performance liquid chromatograph equipped with a diode array and a gamma detector."

Services

  • 25-OH Vitamin D EIA ( Material analysis service )

    Enzyme immunoassay traceable to the ID-LCMS/MS 25(OH)D Reference Method Procedure and Vitamin D Standardization Program certified.

  • Antioxidant Panel ( Analysis service )

    Concentration (micrograms per Liter) of zeaxanthin+lutein, beta-cryptoxanthin, cis-lycopene, trans-lycopene, alpha-carotene, cis-beta-carotene, trans-beta-carotene, retinol, delta-tocopherol, gamma-tocopherol, alpha-tocopherol measured by HPLC.

  • Apolipoproteins ( Material analysis service )

    Concentrations (mg/dL) measured by ELISA.

  • Blood Lipid Panel ( Material analysis service )

    Triacylglyceride (TG) and total cholesterol by enzymatic assay; HDL cholesterol by apoB precipitation and enzymatic assay; LDL cholesterol by Friedewald equation.

  • Custom Assays ( Material analysis service )

    Available upon request.

  • Fatty Acid Profile ( Material analysis service )

    Percentage of total fatty acid for 42 different fatty acids measured by GC-FID.


Last updated: 2018-08-01T16:43:25.730-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016