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Forsyth Imaging Core

Director: Bidlack, Felicitas, Ph.D.

Location: 245 First Street, 5th Floor Science Center, room 5123, Cambridge, MA 02142

Summary:

The Forsyth Imaging Core specializes in light microscopy for spectral imaging and imaging of clarified tissue samples.

Affiliations:

People:

Resources:

Instruments

  • Image Processing Workstation ( Computer workstation )

    - Work station with the following software:
    - Imaris Software
    - Zen Black
    - Zen Blue
    - FIJI
    - MATLAB

  • Smaller dissecting microscopes ( Stereo microscope )

    Please inquire

  • Zeiss Axio Observer Inverted Microscope ( Inverted microscope )

  • Zeiss Evo LS10 scanning electron microscope ( Environmental scanning electron microscope )

    EVO LS 10 includes:
    -Stage movement of 80x100x35 mm (X,Y,Z)
    -Maximum specimen height of 100 mm

  • Zeiss LSM 780 Confocal Microscope ( Laser scanning confocal microscope )

    34 channel GaAsP detector for spectral imaging

  • Zeiss LSM 880 Confocal Microscope ( Confocal microscope )

    Specialized Tissue Clearing Objectives (20x/1.0, WD=5.6mm) for LSM880

  • Zeiss Stemi SV11 stereo microscope ( Stereo microscope )

    - Zoom factor of 11; the widest range for fully color-corrected, distortion-free images - sharp from edge to edge.
    - Coarse/fine control for rapid, precise focusing.
    - High-intensity, 150 watt coaxial illumination.
    - Antiflex cap for specular surfaces.
    - Largest working distances: ovjective 1x = approx. 80 mm; ovjective 1.6x = approx. 40 mm
    - New type of tube with convenient viewing angle of 35°, allowing you to work without fatigue for longer periods
    - Perfect also for 3-dimensional pictures.

Services

Software

  • Fiji/Imagej ( Software )

    "ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.

    It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.

    It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."

    Available through the NIH.

  • Imaris ( Software )

    "Delivers all the necessary functionality for data visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size, from a few megabytes to multiple gigabytes in size. Conveniently load, process and visualize data and images acquired from almost any confocal and wide field microscope."

  • MATLAB ( Software )

    "MATLAB® is a high-level language and interactive environment that enables you to perform computationally intensive tasks faster than with traditional programming languages such as C, C++, and Fortran."

  • ZEN ( Software )

    Includes Zen Black and Zen Blue


Web Links:

Last updated: 2017-08-29T14:19:08.351-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016