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CyTOF Antibody Resource and Core

Director: Cahill, Laura

Location: Brigham and Women’s Hospital, 20 Shattuck Street, Thorn Building, Room 425, Boston, MA 02115

Summary:

Our core specializes in conjugating purified antibodies to a 35-metal catalogue for CyTOF users. With over 429 unique markers (261 for human and 168 for mouse), any user can create their own panel from our inventory. We also have optimized protocols specific to CyTOF for cell surface, intracellular cytokine, transcription factor, and phospho-signaling stains. All protocols have upstream specific techniques optimized for single cell population preservation following cryopreservation and through the entirety of the staining procedure.

We are akin to flow cytometry, but we actually utilize mass cytometry (CyTOF).

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Protocols

Services

  • Conjugated Antibody Requests ( Material production service )

    For Harvard-affiliated institutions, the price for a standard antibody vial (40 uL) is $70. For custom conjugations, the price is $230 for a vial (200 uL). For institutions not affiliated with Harvard, the respective prices are $101 and $331. Please download a request template from our website for all orders.

  • Panel Design and Staining Protocols ( Support service )

    Knowledge of metal cross-talk and antibody sensitivity/relative protein abundance is used to carefully design each panel. Optimized protocols specific to CyTOF include cell surface, intracellular cytokines, transcription factors, and phospho-signaling stains. If interested, please e-mail Laura Cahill at LCahill5@bwh.harvard.edu.

Software

  • Cytobank ( Algorithmic software suite )

    "Cytometers are able to analyze more than 30 parameters per cell, and millions of cells per sample. Cytobank is built from the ground up on the concept of single-cell analysis, with all data visualizations connected to the individual cell events. This enables unparalleled communication between clinical researchers, scientists, and informaticians that takes full advantage of what cytometry has to offer."


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Last updated: 2017-06-22T13:16:28.061-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016