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HSCI-CHB Flow Cytometry Research Lab

Director: Mathieu, Ronald, Ph.D.

Location: Flowlab at CLS 15037: 3 Blackfan Circle, Flowlab at ENDERS 853: 96 Binney St, Boston MA 0215, Flowlab at KARP 8113: 1 Blackfan Circle, Boston MA 02115

Summary:

The lab is committed to meeting all the flow cytometry research needs of the HSCI, BCH, DFCI and Harvard communities. We are continuously improving our equipment and software to bring the latest instrumentation and capabilities to our users for cell sorting and analysis. Since being established in August of 2008, under the Direction of Ronald Mathieu, Ph.D, ASCP, the lab has contribute to a significant amount of publications and brought forth many collaborations.

Affiliations:

People:

Resources:

Instruments

  • 1-Laser GuavaCyte ( Flow cytometer )

  • 2-Laser FC-500 ( Flow cytometer )

  • 3 Fully equipmed analysis station (FLowjo, Diva, ModFit and Kaluza) ( Flow cytometer analyzer )

  • 3-Laser BL2 BD FACSAria II u ( Flow cytometer sorter )

  • 3-Laser MACSVYB ( Flow cytometer )

  • 3-Laser Moflo Legacy ( Flow cytometer sorter )

    405nm Violet Laser:
    - 450/50 FL7 (PACBLUE)
    - 610/20 FL8 (QDOT605)

    488nm Blue Laser:
    - 530/30 FL1 (FITC)
    - 575/26 FL2 (PE)
    - 610/20 FL3 (PETXRED)
    - 670/30 FL4 (PECY5)

    640nm Red Laser:
    - 670/14 FL5 (APC)
    - 780/60 FL6 (APCCY7)

  • 4-Laser BL2 BD FACSAria II ( Flow cytometer sorter )

  • 4-Laser Fortessa ( Flow cytometer analyzer )

    405nm Violet Laser:
    - 450/50 Pacific Blue
    - 525/50 AmCyan
    - 610/20 Qdot 605

    488nm Blue Laser:
    - 530/30 FITC
    - 575/26 PE
    - 610/20 PE-Texas Red/PI
    - 670/30 PeCy5
    - 695/40 PerCP-Cy5.5
    - 780/60 PE-Cy7

    640nm Red Laser:
    - 670/14 APC
    - 730/45 Alexa Fluor 700
    - 780/60 APC-Cy7

  • 5-Laser LSR II ( Flow cytometer analyzer )

    355nm UV laser intercept:
    - 530/30 Indo-1
    - 450/50 Hoechst Blue

    405nm Violet Laser intercept:
    - 610/20 QDot-605
    - 525/50 AmCyan/ BD Horizon V500
    - 450/50 Pac Blue/ BD Horizon V450

    488nm Blue Laser intercept:
    - FSC
    - SSC
    - 550/20 YFP
    - 530/30 FITC/Alexa 488
    - 695/40 PerCP, PerCP-Cy5.5

    561nm (Yellow/Green) intercept:
    - 780/60 PE Cy7
    - 710/50 PE-Cy5.5
    - 670/30 PE-Cy5
    - 610/20 PE TxRd/mCherry
    - 585/15 PE

    640nm Red Laser intercept:
    - 780/60 APC CY7
    - 730/45 Alexa-700
    - 660/20 APC

  • 6-Laser BD FACSAria II u ( Flow cytometer sorter )

Services


Web Links:

Last updated: 2017-01-11T23:04:13.838-06:00

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