The Cell Biology Microscopy Facility was created to provide the Harvard Medical School basic research community with access to advanced light microscopes with an emphasis on live cell imaging with high spatiotemporal resolution. The CBMF includes a Lattice Light Sheet microscope and an OMX Blaze Structured Illumination Super-resolution microscope. We provide expertise and assistance in experimental design using these new techniques.
Please note: access to services varies with equipment, see access policy on our website.
We are very excited to have the lattice light sheet microscope, designed by Eric Betzig at Janelia Research campus. Light sheet microscopy is a rapidly emerging microscopy technique that provides increased imaging speed and minimal photobleaching and photo-toxicity by using a second objective lens to restrict illumination to the focal plane of the detection objective. The lattice light-sheet microscope uses a linear array of coherent Bessel-Gauss beams to create an extremely thin light sheet that is tightly confined in the axial (Z) direction. The result is unprecedented spatiotemporal resolution when imaging live samples in three-dimensions over time, while minimizing photo-toxicity and photobleaching.
- Twofold increase in lateral (120nm) and axial (340nm) resolution with 3D Structured illumination
- Fast 3D-SIM: 1 μm Z-stack (120 images) in as fast as 1 frame per second (at 1ms exposure)