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Glycomics Core (BIDMC)

Director: Lehoux, Sylvain, Ph.D.

Location: Beth Israel Deaconess Medical Center, 330 Brookline Avenue, room SL-0417, Boston, MA 02215

Summary:

The Glycomics Core provides services, instrumentation and expertise in glycomics to BIDMC research groups, affiliated and non-affiliated institutions and corporate companies.

Affiliations:

People:

Resources:

Instruments

Services

  • Consulting Service ( Support service )

    Consult with the Glycomics Core team to move your glycomics project(s) forward. The Glycomics Core can provide assistance with experimental and project design, grant proposal, research and data interpretation.

  • Distribution of Reagents ( Transport service )

    - Divers reagents (antibodies, cell lines, etc…) are made available to the research community only for the cost of shipping and handling. Reagents are shipped overnight on dry ice or ice packs.

  • Glycan Microarrays ( Material analysis service )

    - Analysis of potential glycan-binding proteins (GBPs) and antibodies, inactivated bacteria or virus on various glycan arrays including CFG glycan array, microbial glycan array, NCFG-derived defined or shotgun arrays
    - Printing of custom microarrays on glass slides using natural occurring glycans, commercially available or glycopeptides/glycoproteins.

    Glycan microarray analysis is currently processed following NCFG and CFG request procedures. More information can be found on their respective website. It will be requested from investigator to briefly describe and explain the nature of their projects.

    Microarray printing service is also available, whether you need to print glycans or peptides/proteins.

  • Mass Spectrometry ( Material analysis service )

    N-glycan and O-glycan profiling of glycoproteins – purified glycoproteins, serum/plasma, cells or tissues by MALDI- (Matrix-Assisted Laser desorption/Ionization) and other related technologies.

    Sialic acid linkage analysis - discriminate between α2,3-linked sialic acid and α2,6-linked sialic acid by MALDI-TOF.

  • Production of Recombinant Glycosyltransferase ( Material production service )

    Several enzymes are currently available, including β4GalT, Fut6, ST3Gal4 and ST6Gal1.

    Although the quantity of material provided to the core facility can be discussed on a case by case basis we propose the following guideline:
    - Purified glycoprotein: 50 µg
    - Serum: 20 µl
    - Cells: 5.10^6 cells
    - Tissue: 50 mg


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Last updated: 2019-02-11T08:02:56.219-06:00

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