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Boston Children's Hospital Viral Core

Director: He, Zhigang, Ph.D.

Location: 3 Blackfan Circle, Center For Life Science Building, Room13060, Boston, MA, 02115

Summary:

Viral Core’s goal is to provide scientists with access to cutting-edge viral vector technologies in an effective, high-quality and cost-efficient fashion. Viral Core also provides consultation to investigators in selecting, designing and using viral vectors.

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Instruments

Services

  • Custom made AAV prep ( Material processing service )

    We purify AAVs using Iodixanol gradients and ultracentrifugation. We could prep AAV vectors into serotype 1, 2, 5, 6, 7, 8, 9, rh10 and DJ. For a regular AAV prep, we need at least 150ug maxi-prep plasmid DNA. Typical virus yield is between 250-400ul. Genomic titer is quantified using Q-PCR. Purified AAV is good for in vivo applications.

  • Custom made lenti prep ( Material processing service )

    Our lenti virus is pseudotyped with vsvg. We concentrate lenti virus by ultracentrifugation and quantify its genomic titer by Q-PCR.
    Please provide at least 100ug plasmid DNA for a lenti prep. Typical virus yield is between 150-200ul.

  • Pre-made AAV prep ( Material processing service )

    We provide pre-made AAVs with different serotypes for AAV-GFP, AAV-Cre and AAV-ChR2. For detailed information of in stock pre-made AAVs, please email chen.wang@childrens.harvard.edu.


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Last updated: 2015-12-16T12:51:18.711-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016