The Assay Development and Screening Facility (ADSF) provides consultation and implementation of high-throughput and high-content screening assays on a fee-for-service model. The core has specialized equipment to support image-based and live cell assay development. The ADSF also partners with the Human Neuron Differentiation Service to create and conduct phenotypic screening using patient derived iPSC lines differentiated into specific neuronal cell types to model human CNS disorders.
Role: Research Assistant, ADSF
The ADSF is anchored by a Hamamatsu FDSS7000EX kinetic reader, which has the capability of conducting measurements of fluorescence or luminescence in 384 wells at once, with time resolution of up to 40Hz. The equipment allows for measurements of changes in membrane potential and concentration of intracellular ions such as Ca2+ in primary cultures, stem cell-derived or trans-differentiated cells or cell lines in a fast, accurate, parallel fashion.
We have a confocal based high-content screening (HCS) platform, ThermoFisher's Arrayscan XTI. This HCS instrument features a temperature and CO2 controlled live cell chamber to assay your biology over several days. The arrayscan XTI has an integrated liquid handler to administer your substrate of interest, well by well, capturing changes in fluorescence upto 10Hz with an enhanced CCD camera and 7 LED light source using 6 to 1536 well labware.
The core enables researchers to develop assays of their targets suitable for subsequent industrial-scale, high throughput screening, thereby filling this critical gap in the drug development pathway. Additional funding by Mass Life Sciences (MLSC) enables access to the core facilities for external and industrial researchers.
The core specializes in live cell screening capabilities not available in other core facilities. It also provides access to annotated NIH and FDA approved screening libraries, as well as chemically diverse sets of small drug-like compound libraries.
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