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Molecular Medicine Core (BIDMC)

Directors: Balk, Steven, M.D., Ph.D.; Petkova, Victoria, Ph.D. (Co-Director)

Location: 330 Brookline Avenue, Beth Israel Deaconess Medical Center, SL232-The Slosberg Building, Boston, MA 02215

Summary:

The core is equipped with the following instruments: Illumina MiSeq Next Generation Sequencer, Arcturus XT Laser Capture Microdissection System, Agilent 4200 TapeStation Bioanalyzer, Covaris E220 Ultra-Sonicator, ABI 7000 and 7500 Fast Real-Time qPCR systems, Li-Cor Odyssey System Infrared Imager.

On an hourly fee basis we provide: training on equipment usage, consultations, core setup, data analysis, troubleshooting, primer design, LCM usage. On per sample or per run fee basis we provide: qRT-PCR runs, quality and quantity DNA and RNA analysis, Covaris ultrasonicator sample shearing, LiCor scanning.

For details contact Dr. Petkova.

Affiliations:

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Instruments

  • ABI 7000 Real-Time PCR System ( Real-time PCR machine )

  • ABI 7500 Fast Real-Time qPCR system ( Real-time PCR machine )

  • Agilent 2200 TapeStation Bioanalyzer ( Capillary electrophoresis instrument )

    This quality and quantity control bioanalyzer is a powerful tool for accurate measurement of your DNA or RNA sample and will get you ready for your next step of application. The DNA ScreenTape will provide you with your sample’s QC needed in NGS, cDNA libraries preparation, genotyping, QC of qPCR products, PCR fragment analysis, mycoplasma detection. The RNA ScreenTape analysis will facilitate the preciseness of your gene expression profiling, qRT-PCR, NGS or microarray experiments.

  • Arcturus XT Laser Capture Microdissection System ( Laser capture microdissection microscope )

  • Covaris E220 Ultra-Sonicator ( Sonicator )

    The Adaptive Focused Acoustics (AFA) technology employs a very high frequency ultrasound waves to be focused into a discrete zone in the sample vessel. This efficient delivery requires minimal energy avoiding the adverse effect on the sample. The focused ultrasonicator provides exquisite control and tuning of the power, making the application of the instrument versatile: from gentle, low-power to high-power applications such as chromatin shearing, DNA fragmentation, liposome and nanosuspension formation.

  • Illumina MiSeq Next Generation Sequencer ( DNA sequencer )

    "Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths."

  • Li-Cor Odyssey System Infrared Imager ( Infrared image acquisition device )

    This Odyssey Infrared Imager is a self service scanner available during for scanning blots.

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Last updated: 2017-03-13T12:44:25.239-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016