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Woolf Lab

Summary:

Dr. Woolf’s laboratory is currently devoted to investigating how the functional, chemical and structural plasticity of neurons contributes to adaptive and maladaptive functions of the mammalian nervous system. The group’s major efforts are devoted to the study of pain, the formation of neural circuits during development, and the failure of regeneration in the adult CNS. Most of this work is focused on primary sensory and spinal cord neurons, which are studied using a multidisciplinary approach that spans mouse and human genetics, molecular and cell biology, bioinformatics, synaptic electrophysiology, neuroanatomy, integrative systems biology and behavior. The group works closely with a wide number of academic groups and the pharmaceutical and biotechnology industry to identify and validate molecular targets for novel analgesics and axonal growth determinants. The lab represents a complex mixture of basic and translational neuroscience.

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Organisms and Viruses

  • Adv-Cre Mouse ( Mus musculus )

    Cre line

  • AMPAR4 KO Mouse ( Mus musculus )

  • ATF3 OE ( Mus musculus )

    Atf3 Flox conditional knockin
    Flox knock-in over expressor

  • ATF3-flox Mouse ( Mus musculus )

    Atf3 Flox conditional knockout

  • BAX KO Mouse ( Mus musculus )

    Bax full knockout

  • BrnIIIa-Cre ( Mus musculus )

    BrnIIIa-CRE line

  • CD1/WDR KO Mouse ( Mus musculus )

    CD1/WDR full knockout

  • Cd11b-thymidine kinase mouse ( Mus musculus )

    Cd11b-thymidine kinase knock-in
    Kills microglia

  • Coronin KO Mouse ( Mus musculus )

    Coronin full knockout

  • fGRIN Mouse ( Mus musculus )

    fGRIN1 Flox conditional knockout
    Flox NR1 subunit

  • GCH1 OE Mouse ( Mus musculus )

    Gch1 flox conditional over-expressor

  • GCH1-eGFP Mouse ( Mus musculus )

    Reporter Line

  • GCH1-flox Mouse ( Mus musculus )

    GCH1 Flox knockout

  • GFRP OE Mouse ( Mus musculus )

    GFRP over expressor Flox conditional Knockin

  • HSP flox Mouse ( Mus musculus )

    HSP Flox conditional knockout

  • HSP OE Mouse ( Mus musculus )

    HSP Flox knock in over expressor

  • Metallotheinen KO Mouse ( Mus musculus )

    MT1 full knockout

  • PACS KO Mouse ( Mus musculus )

    PACS full knockout

  • Parv-Cre Mouse ( Mus musculus )

    CRE line

  • Rag KO Mouse ( Mus musculus )

    Rag full knockout

  • Rosa-Diptheria Toxin A-flox Mouse ( Mus musculus )

    Rosa-Diptheria Toxin A-floxed

  • Rosa-Tomato Mouse ( Mus musculus )

    ROSA26-Tomato flox reporter line

  • Rosa-Zgreen Mouse ( Mus musculus )

    Rosa-Zgreen floxed reporter line

  • Sema5a KO Mouse ( Mus musculus )

    "In this knockout strain, exon 6 of the semaphorin 5A (Sema5a) gene is excised (includes coding region), abolishing gene function. Sema5A is a transmembrane protein which acts as a repellant for axonal guidance during retinal neural development. Wildtype P0 and P3 mice express Sema5A in the outer neuroblastic layer, directly adjacent to the inner neuroblastic layer (INBL). At P7, P10, and P14, the expression is only observed in the middle to outer part of inner nuclear layer (INL), and transcripts are not detectable at P21, when retinal development is almost complete. Homozygotes are viable and fertile. When bred to mice lacking Sema5b (Stock No. 017534), SEMA5A/B double KO mice exhibit severe defects in retinal ganglion cells, amacrine cells, cholinergic amacrine cells, calretinin-positive amacrine cells, and calbindin-positive amacrine cells. The defects lead to neurite mistargeting within both the inner plexiform layer (IPL) and INL resulting in functional defects in retinal responses to visual stimuli. These mice may be useful for studying axonal guidance and lamination regulation during retinal development. " Sema5a knockout

  • SNS-Cre Mouse ( Mus musculus )

    SNS-CRE line

  • SOD-(G93A) ( Mus musculus )

    Transgene has G93A point mutation ocuring in humans

  • T cell receptor α/d KO Mouse ( Mus musculus )

    T cell receptor α/d knockout

  • Thy1-YFP Mouse ( Mus musculus )

    Thy1-YFP flox reporter line

  • TRPA1 KO Mouse ( Mus musculus )

    TRPA1 knockout


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Last updated: 2013-09-04T14:15:01.012-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016