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Eggan Lab

Summary:

In my laboratory, we pursue two interlocking areas of investigation: the basic biology of stem cell programming and reprogramming, as well as the application of the resulting technologies to studies of the neuromuscular system and the diseases that affect it.

Coming to a fundememtal understanding of how a cell's identity is determined during differentiation and how it can in turn be manipulated experimentally, is a central goal of developmental biology, one with susbstantial ramifications for biomedicine. We study both the differentiation of embryonic stem cells into the neural lineage and the reprogramming of commonly available differentiated cell types, such as fibroblasts, into either pluripotent stem cells or cells of therapeutic interest such as spinal motor neurons. To study differentiation and dedifferentiation, we employ a variety of approaches including stem cell differentiation, nuclear transfer and defined reprogramming strategies using known transcriptional regulators and novel small molecule compounds.
A number of devestating diseases, including ALS and SMA specificaly affect the neuromuscular system. Little is known concerning the molecular pathology underlying these conditions at least in part because it has been impossible to access significant quantities of the disease affected cell type, the spinal motor neuron. With recent advances in stem cell and reprogramming biology we can now produce billions of spinal motor neurons with control and diseased genotypes. We use this new resource to design in vitro disease models for both mechanistic studies and for the discovery of novel small molecule therapeutics.

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Reagents

  • 11a - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB and teratomas

  • 11c - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB and teratomas

  • 17b - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB and teratomas

  • 29a -ALS [SOD1(L144F)] - Female ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB

    Genetic Disorder: (ALS) Amyotrophic Lateral Sclerosis (L144F [Leu144 > Phe] dominant allele of the superoxide dismutase (SOD1) gene)

  • 29b - ALS [SOD1(L144F)] ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB

    Genetic Disorder: (ALS) Amyotrophic Lateral Sclerosis (L144F [Leu144 > Phe] dominant allele of the superoxide dismutase (SOD1) gene)

  • A29c - ALS [SOD1(L144F)] ( Induced pluripotent stem cell line )

    Pluripotency Tested:
    3 germ layers in EB

    Genetic Disorder: (ALS) Amyotrophic Lateral Sclerosis (L144F [Leu144 > Phe] dominant allele of the superoxide dismutase (SOD1) gene)


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Last updated: 2013-11-04T08:26:59.440-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016