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HSCI Humanized Neonatal Mouse Center

Director: Marasco, Wayne A., MD, PhD

Summary:

There is a growing need for animal models to carry out in vivo developmental and regenerative medicine studies of human cells, tissues and organs. The Humanized Neonatal Mouse Center (HNMC) was created to accelerate research in the stem cell field by providing humanized mouse models to study human stem cell engraftment and differentiation in regenerative medicine. We have over 4 years of experience in constructing different types of humanized mouse models, including neonatal heart, lung and kidney injury models. We have extensive experience in hematopoietic stem cell reconstruction. It is our goal at HNMC to facilitate collaborative research in human stem cell biology, where physiologically relevant microenvironments (niches) may be created in vivo to study human stem cell fate and function under experimental settings where disease, damage or degenerative conditions can be controlled. We can provide customized humanized mouse models to the HSCI research community, to collaborate on research studies of common interest, and to advance the general use of these models for a broad range of translational and preclinical studies.

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Organisms and Viruses

  • BLT humanized mouse ( Mus musculus )

    "BLT: The BLT mouse is a modification of the SCID-Hu model which was developed by Joseph McCune in the early nineties. To construct the BLT model, NOD/SCID mice are cotransplanted with human liver and thymus tissues along with autologous CD34+ hematopoietic stem cells. Development of a robust human hemato-lymphoid system is observed between 12-16 weeks post engraftment with human cells and tissues. This model is also being used extensively in HIV research and studying of virus-specific human immune responses. The BLT mice are, however, susceptible to thymic lymphoma and may not allow long term studies (average lifespan ~8.5 months)."

  • GTL humanized mouse ( Mus musculus )

    "GTL: This mouse model, also known in the literature as NSG-BLT, is a modification of the BLT mice where cotransplantation is performed on the NSG and not on NOD/SCID background. Since the lifespan of the NSG mice is not curtailed by the development of thymic lymphomas as in the NOD/SCID, the GTL mouse model would potentially allow researchers to carry out longer term experiments than is not possible with the BLT mice."

  • hNSG humanized mouse ( Mus musculus )

    "hNSG: NSG (NOD/SCID IL-2 receptor gamma chain knockout, Jackson Laboratories, stock#005557) mice engrafted with human CD34+ hemopoeitic stem cells demonstrate high level reconstitution with human immune cells. The humanized NSG (hNSG) mice can be used to set up small animal models of human infectious diseases and test the efficacy of antimicrobials. This mouse model is being extensively used to establish HIV infection models and testing of various therapeutic strategies involving the use of siRNA, zinc finger nucleases and topical microbicides. This model has also been used to study various cancers of the human immune cells including AML, ALL and Multiple Myeloma. However, it is to be noted that human T cell development in this mouse model is delayed which calls for further modifications/enhancement of this model."


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Last updated: 2014-02-24T10:00:11.052-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016