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Lederer Lab

Location: Brigham and Women's Hospital, Vascular Surgery, 75 Francis St, Boston, MA 02115

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    Organisms and Viruses

    • Caspase-1-KO ( Mus musculus )

      "Mice homozygous for the Il10 and Casp1 targeted mutations are viable and fertile when housed under SPF conditions. NOD/Lt mice deficient for both of these genes develop type 1 diabetes at a rate equivalent to the parental strains. The Il10 targeted mutation also renders this NOD/Lt stock susceptible to colitis (although not as severe as other strains of Il10 deficient mice) when maintained under standard housing conditions."

    • CD4-KO ( Mus musculus )

    • Head injury model ( Mus musculus )

      Model of: Craniocerebral Trauma

    • MyD88-KO ( Mus musculus )

      homozygous null strain for Myeloid differentiation primary response gene (88) in C57Bl/6 background

    • OTII TCR tg ( Mus musculus )

      "These transgenic mice express the mouse alpha-chain and beta-chain T cell receptor that pairs with the CD4 coreceptor and is specific for chicken ovalbumin 323-339 in the context of I-A b. Homozygous mice are viable and fertile. In these mice there is a four-fold increase in the CD4 to CD8 peripheral T cell ratio, and lymph node T cells demonstrate a dose-dependent proliferative response to the specific ovalbumin ligand. These transgenic mice are useful for studying in vivo T cell biology such as TCR-ligand interactions, T cell activation, thymic selection, cross-presentation of antigens, process of thymic selection and central and peripheral T cell tolerance and induction."

    • Peritoneal infection model ( Mus musculus )

    • Pneumonia Model ( Mus musculus )

      Lung Pneumonia model using Pseudomonas aeruginosa, or Streptococcus pneumoniae

    • Sepsis model ( Mus musculus )

      Cecal ligation and puncture procedure for induction of Sepsis (CLP).

    • TIRF-KO ( Mus musculus )

      "Mice that are homozygous for the mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This single base pair deletion mutation was induced by ENU mutagenesis. Unlike wildtype macrophages, macrophages derived from these animals fail to respond to synthetic lipid A, endotoxin lipopolysaccharide (LPS), and dsRNA with production of tumor necrosis factor (TNF). Macrophages are less susceptible to LPS-induced cytotoxicity. Nitrous oxide and type I interferon production in activated macrophages is impaired. Homozygotes exhibit increased susceptibility to mouse cytomegalovirus. Although homozygotes are resistant to challenges with LPS, the mice will become ill and some may die."

    • Total body radiation ( Mus musculus )

    • Trauma Model ( Mus musculus )

      Model of: Closed Head Injuries, Craniocerebral Trauma

    Protocols


    Last updated: 2013-03-14T10:41:01.152-05:00

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    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016