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Ragon Institute Imaging Core, Flow Cytometry (MGH)

Director: Waring, Michael T.

Location: 400 Technology Square, 9th floor, Cambridge, MA, 02139

Summary:

The mission of the Ragon Institute Imaging Core is to bring the latest imaging modalities to bear on fundamental molecular and cell biological questions pertaining to infectious diseases. As an MGH core, it also serves the greater MGH community in all aspects of microscopy, flow cytometry and cell sorting. The Flow Cytometry division of the Ragon Institute Imaging Core is located on the 9th floor of 400 Technology Square in Cambridge. The core offers training, assistance and access to flow cytometers and other instrumentation. Consultation is provided on experimental design. Our high speed cell sorter is situated in a BL2+ facility, permitting sorting of fixed or live samples with up to BL2+ level of biosafety.

Since the Ragon Institute is also a collaboration between MGH, Harvard, and MIT, so too does the facility welcome members of the MIT and Harvard communities.

Affiliations:

People:

Resources:

Instruments

  • 3 Laser LSRII (BD Biosciences) ( Flow cytometer analyzer )

    405, 488 and 640 excitation lines
    16 colors
    High Throughput Sampler (HTS)

  • 4 Laser, 13 color LSR II (BD Biosciences) ( Flow cytometer analyzer )

    350, 405, 488, and 640 excitation lines
    13 colors

  • 4 Laser, 18 color LSR II (BD Biosciences) ( Flow cytometer analyzer )

    350, 405, 488, and 640 excitation lines
    18 colors

  • Analysis Stations ( Computer workstation )

    Access to Flowjo Software and other tools

  • AutoMACS cell separator (Miltenyi Biotech) ( Cell separator )

    "With the autoMACS® Pro Separator you can isolate cells fully automated, directly from whole blood or bone marrow, which makes it ideal for multiple myeloma research or lineage-specific chimersim analysis after bone marrow transplantation. autoMACS technology hast been cited in over 2300 peer-reviewed publications, making it the most trusted, most proven automated magnetic cell separation technology."

  • Bioplex 3D multiplex analyzer (Bio-Rad) ( Microarray platform )

    "The Bio-Plex® 3D suspension array system is the next generation multiplexing platform based on xMAP technology. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for high-throughput testing for nucleic acid and protein applications.
    Measure up to 500 unique analytes in a single sample
    96- and 384-well plate capability
    Plate read times twice as fast as the Bio-Plex/Luminex 200 system
    Robotics interfacing capabilities
    LIS-compatible software
    Compatible with the magnetic and nonmagnetic assays
    Bio-Plex Manager™ software version 6.0 for data analysis"

  • Sony SH800 Cell Sorter (Sony Biotech) ( Flow cytometer sorter )

    "The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes and applications using the 70 μm, 100 μm, and 130 μm microfluidics sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to use.

    System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that also can be exported to third party analysis tools."

  • SORP FACSAria II (BD Biosciences) ( Flow cytometer sorter )

    350, 405, 488, 561, 640 excitation lines
    18 colors

  • Two 5 Laser LSR Fortessa (BD Biosciences) ( Flow cytometer analyzer )

    350, 405, 488, 561, 640 excitation lines
    18 colors
    HTS

Services

  • Assisted Flow Cytometry ( Material analysis service )

    Users may have assistance in the use of the flow cytometers.

  • Flow Cytometric Cell Sorting ( Material modification service )

    Users can book time with core personnel to have their samples run on a cell sorter to purify target populations of interest. The FACSAria IIu sorter is housed in a biosafety cabinet to allow for safe processing of primary human samples or infectious samples. Up to 4 populations can be selected at a time.

  • Flow Cytometry Instrumentation Access ( Access service )

    Trained users are permitted to sign up for and use the flow cytometers for analysis at their convenience.

  • Flow Cytometry Training ( Training service )

    The core offers a training regimen to prepare researchers to become independent operators of our instrumentation. The full syllabus is outlined on the “Training” page found via the link below, and includes an “Introduction to Flow Cytometry” seminar and hands-on instruction in instrument operation and optimization.


Web Links:

Last updated: 2018-02-02T16:11:57.183-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016