Provides resources in immunofluorecence, electron microscopy, confocal, and deconvolution microscopy of living and fixed cells and tissues, and offers expertise in imaging polarized cells in monolayer culture
There are 3 locations for Core B:
* Immune Disease Institute (IDI)
* Children's Hospital Boston (CHB)
* Beth Israel Deaconess Medical Center (BIDMC)
At the CHB location only.
CHB location contains two Nikon E800 microscopes, one with a Bio-Rad MRC1024 confocal laser scanner.
Used for DIC, phase and fluorescence microscopy interfaced to a spinning disc confocal at the IDI location.
Facility has four of these available at the CHB location.
Used for DIC, phase, fluorescence and Apotome-based confocal microscopy at the CHB location.
Used for phase, fluorescence and spinning-disk confocal microscopy at the CHB location.
Used for phase, fluorescence and TIRF microscopy.
Used for DIC, phase, and fluorescence microscopy at the IDI location.
At the IDI location only.
The Imaging Core makes available to the research community modern motorized microscopes capable of multiple modes of imaging of both live and fixed biological samples. In particular, our optical microscopes can perform phase and DIC imaging, widefield fluorescence, ratio imaging, laser scanning and spinning-disk confocal microscopy, dual-color total internal reflection microscopy, 5-D fluorescence imaging, FRAP, photoconversion and FRET imaging.