eagle-i Harvard UniversityHarvard University
See it in Search

Animal Physiology Core (JDC)

Director: Goodyear, Laurie, Ph.D.

Location: Joslin Diabetes Center, One Joslin Place, Boston, MA 02215

Summary:

The Joslin Animal Physiology Core provides technically advanced physiological evaluation of rodents. The core has non-invasive devices for the study of metabolism under conditions of exercise, diabetes, obesity, and unique diets. In addition, the core has 2D and 3D in vivo optical imaging for the measurement of fluorescence, bioluminescence and microCT. The facility at Joslin enables external users to access the core via an associated quarantine room for the importation of mice. Core personnel offer their expertise in designing and executing a variety of physiological assessments. For any inquiries about Core services, please contact Core personnel.

Affiliations:

People:

Resources:

Instruments

  • Comprehensive Lab Animal Monitoring System (CLAMS) ( CLAMS system )

  • Diurnal Incubators ( Incubator )

    Used to study the physiological mechanisms of brown fat generation and activation at both thermoneutral and cold temperatures.

  • DSI Telemetry System (body temperature) ( Telemetry system )

    "Scientists are able to study a number of physiological parameters in laboratory mice using DSI PhysioTel™ Transmitters. DSI offers different implantable transmitters to measure different parameters including pressure and activity; temperature and activity; biopotentials and activity; as well as transmitters that measure a combination of pressure, biopotentials, temperature and activity.

    DSI implantable telemetry transmitters have been, and continue to be, used in studies involving mice such as telemetric recordings of sleep and home cage activity, abnormal heart rate and body temperature in mice lacking thyroid hormone receptor x1, the effects of triiodothyronine on heart rate, and numerous other studies

    All of DSI’s PhysioTel Transmitters are implantable and capable of measuring activity. The design of the transmitter shape allows for subcutaneous or intraperitoneal placement and PhysioTel transmitters can be re-sterilized between uses, extending their implant life and reducing costs."

  • Dual Energy X-ray Absorptiometry scanner ( Densitometer )

  • IVIS SpectrumCT imaging system ( Computed tomography scanner )

    "The IVIS® SpectrumCT offers the ultimate performance and range of applications for in vivo imaging – leading edge molecular AND anatomical imaging in a single integrated system. The IVIS SpectrumCT was designed to offer the ultimate range of applications for in vivo and longitudinal imaging. The system enables synchronization of functional and anatomical data simultaneously and co-registered for true quantitative 3D imaging.

    The IVIS SpectrumCT maintains the versatility and the most advanced optical features similar to the world leading imaging platform, the IVIS Spectrum. Moreover, the IVIS SpectrumCT offers low dose and ultrafast microCT for multiple animals, simultaneously. The average dose per scan is about 10mGy with scanning and reconstruction times of less than a minute."

  • Laser Doppler Perfusion Imaging system ( Laser doppler flowmeter )

  • Metabolic Cages ( Animal cage )

  • Non-Invasive Tail Cuff Blood Pressure System ( Blood pressure monitor )

  • Rodent Exercise Treadmills ( Treadmill )

Services

  • Blood Pressure Monitoring ( Material analysis service )

    The Animal Physiology Core personnel use non-invasive tail-cuff sensor and monitoring system for the measurement of systolic and diastolic blood pressure and heart rate.

  • Body Composition using DEXA ( Material analysis service )

    The Dual Energy X-ray Absorptiometry (DEXA) scanner uses x-rays to assess lean and fat mass, bone mineral density and bone length. The DEXA scanner (Lunar PixiMus II) can be used on mice up to 55 grams in weight.

  • Energy Expenditure and Metabolism using the CLAMS system ( Material analysis service )

    The 12 chamber CLAMS system measures physiologic parameters including oxygen consumption (VO2), carbon dioxide (CO2) production, respiratory exchange ratio (RER), food and drinking behavior, activity level, and caloric heat production.

  • Exercise Procedures ( Material analysis service )

    The Animal Physiology Core has treadmills and wheelcages for acute and long-term training. After training in their use, the researcher will use these independently.

  • IVIS Spectrum CT Imaging System Monitoring ( Material analysis service )

    A new IVIS Spectrum CT imaging system is now being added to the Animal Physiology Core as part of the current expansion. This system uses novel optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and regulated gene expression patterns in living animals via bioluminescent and fluorescent reporters.

  • Metabolic Cages for By-product Collection ( Material processing service )

    The Metabolic Cages are used to collect by-products from an individual rodent as each chamber accurately separates urine from feces.

  • Physiological Measurements using Telemetry System ( Material analysis service )

    The DSI Telemetry System uses implantable transmitters to measure a number of physiological parameters in mice. Some of these parameters include, but are not limited to, blood pressure and activity, heart rate and activity, and body temperature and activity - all of which are measured telemetrically from the appropriate transmitter inside the individual mouse.

  • Temperature Control Diurnal Chambers ( Material analysis service )

    The Core has 2 diurnal incubator chambers (Caron) to study physiological mechanisms in mice at thermoneutral and cold temperatures. The chambers can hold up to 12 cages each and are set to the standard light cycle.

  • Vascular Studies ( Material analysis service )

    A Laser Doppler Perfusion Imaging (LDPI) system is used to provide functional evidence for ischemia-induced changes in vascularization.


Web Links:

Last updated: 2019-03-11T11:50:02.840-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016