The Microbial Identification Microarray Core (MIM) at The Forsyth Institute is a one-of-a-kind core service that enables the rapid determination of bacterial profiles of human clinical samples. The first MIM offering focuses on the detection of bacterial profiles from clinical samples from the oral cavity. Drs. Bruce Paster and Floyd Dewhirst, have used molecular analyses based on 16S rRNA sequencing to identify about 600 oral bacterial species, of which over half have not yet been cultivated. Using this information, they have developed the Human Oral Microbe Identification Microarray, or HOMIM, which allows the simultaneous detection of about 300 of the most prevalent oral bacterial species, including those that cannot yet be grown in vitro.
Microarrays targeting bacterial species of the human and mouse intestines are presently under development. In addition, exploratory and pilot studies to identify bacteria within any human clinical sample by 16S rRNA cloning and sequencing are available.
This service is available to researchers from all academic institutions and to industry. Researchers submit DNA isolated from clinical samples and receive an online comprehensive data analysis and easy-to-interpret readout. Depending upon the number of samples to be analyzed and position in the queue, results can usually be obtained within days. Note that results are presently for research purposes only.
"The following procedure is suitable for isolation of DNA from hard-to-lyse species, such as S. mutans, Staphylococcus spp., Actinomyces spp., and Scardovia spp. This procedure works well for samples from all oral sites including saliva, supragingival or subgingival plaque, buccal epithelial cells, scrapings from tongue surfaces, palatal and throat swabs, and denture surfaces."
The Human Oral Microbe Identification Microarray, known as HOMIM, allows for the simultaneous detection of about 300 of the most prevalent oral bacterial species, including those that have not yet been cultivated. HOMIM is recognized as a valuable research tool by many investigators from academic and private institutions, government, and industry.
Determination of bacterial profiles of human clinical samples and comparison of bacterial associations in health vs. disease.
Investigating for the presence of oral bacterial species in extra-oral sites such as abscesses, atherosclerotic plaques, sepsis, etc.
Researchers submit DNA isolated from clinical samples and receive an online comprehensive data analysis and easy-to-interpret readout.
"Utility of HOMIM:
- To determine and compare bacterial associations in oral health and disease, including different types of periodontitis, caries, gingivitis, ventilator-associated pneumonia, endodontic and odontogenic lesions, abscesses, and halitosis
- To determine the efficacy of therapies, e.g., mouth rinses, antibiotic treatment, scaling and root planing, and laser or periodontal surgery
- To determine the progression of oral diseases
- To determine those patients at risk for periodontitis and other oral diseases
- To determine potential biomarkers for non-oral, systemic diseases, e.g., pancreatic cancer, heart disease, and Crohn's Disease
HOMIM has been used to rapidly produce preliminary data for grants, to obtain data for student projects or theses, and to analyze precious, archived frozen samples (e.g., saliva).
At this time, HOMIMs may not be used for diagnostic purposes and are to be used only for research endeavors."
We test for the effects of various therapies on the oral ecology.