Genomics Core: High-density oligonucleotide arrays allow quantitative analysis of thousands of transcripts and provide an efficient way to determine the full pattern of alterations in gene expression present in a wide variety of experimental conditions and pathologic states. Affymetrix chips are high-density collections of 25-mer oligonucleotides aligned in arrays on a silicon chip. We also provide RNA quality assessment through Bioanalyzer assays.
PCR Core: The PCR Core is part of the Genomics Core and maintains a suite of real-time PCR instruments.
Metabolic Core: The Genomics Core also houses XFe96 and XF24 Seahorse Analyzers for the real-time measurement of metabolism in live cells.
"The 7900HT Fast Real-Time PCR System is the only real-time quantitative PCR system that combines 384-well plate compatibility with fully automated robotic loading—and now also offers optional Fast real-time PCR capability. Acknowledged as the gold standard in real-time PCR, the 7900HT system combined with TaqMan Genomic Assays enables you to achieve unprecedented throughput and flexibility, allowing you to pursue projects beyond the scope of previous real-time instruments."
"The ABI prism 7900HT Sequence Detection System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An Automation Accessory combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of todays drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5 nuclease assay."
"The GeneChip® Fluidics Station 450 is used for the wash and stain operation of Affymetrix GeneChip® arrays. The instrument includes design advancements to provide improved ease of use and true walk-away freedom dramatically improving efficiency in your genetic analysis."
"The GeneChip Hybridization Oven 645 provides precise temperature and rotation control to ensure the successful hybridization of as many as 64 GeneChip cartridges at one time.
An ergonomic front-panel display contains system status indicators and buttons for adjusting the set point temperature and the rotisserie speed. This panel, in addition to a highly visible display and large window, provides immediate feedback of proper operation when using the oven.
The oven can be configured for 110 or 220V operation."
"The GeneChip Scanner 3000 incorporates advanced design improvements with space-saving, speed, superior performance, and lower scanner-to-scanner variation to dramatically improve throughput and efficiency in your genetic analysis. This new scanner provides more accurate gridding and more consistent scanner-to-scanner biological performance, improving data integrity and data sharing between researchers."
"The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells on a single platform. Results are delivered within 30-40 minutes in automated, high quality digital data.
The BioAnalyzer can be used for rapidly analyzing RNA, DNA, and proteins. This instrument uses lab-on-a-chip technology to perform automated quality control, quantification, and sizing of samples. Applications include: Microarray sample quality control, PCR optimization, Analysis of protein purifications."
"The MJ Research PTC-200 is a 96 well gradient thermocycler that can be supplied with different alpha blocks such as a 96 well or a dual 48-well block."
"The Seahorse XFe24 Analyzer measures OCR and ECAR of live cells in a 24-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis and provide a systems-level view of cellular metabolic function in cultured cells, islets, and ex vivo samples."
"The Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis and provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples."
We perform analysis of RNA samples using the RNA Nano assay.
In-house bioinformatics team can provide analysis for microarrays.
Assistance with sample preparation (RNA isolation), experimental design, and choice of array.
Supported arrays include Affymetrix 3' expression arrays, transcriptome arrays, gene arrays, exon arrays, and promoter arrays. We perform microarray assays from total RNA, but also perform hybridization of user-prepared cRNA/cDNA samples to experimental arrays. Preliminary analysis of data is done with Affymetrix Expression Console.
Instruction in RT-PCR setup, operation of SDS software, and operation of PCR machines.
Training on Seahorse instrumentation for new users, with ongoing troubleshooting support.
"The Primer Express® Software v3.0 allows you to design your own primers and probes using TaqMan® and SYBR® Green I dye chemistries for gene quantitation and allelic discrimination (SNP) real-time PCR applications. Developed specifically for use with our 7300, 7500, 7500 Fast, and 7900HT Fast Real-Time PCR Systems, Primer Express® Software provides customized application-specific documents for absolute/relative quantitation and allelic discrimination."
"Affymetrix® Expression Console software supports Probe Set summarization and CHP file generation for both 3' Expression (GeneChip® Human Genome U133 Plus 2.0 Array) and Exon Arrays (GeneChip Human Exon 1.0 ST Array)."