The Flow and Imaging Cytometry Resource provides research flow and imaging cytometry services to all investigators in the PCIMM at Children's Hospital, Boston and Immune Disease Institute, HMS and the local scientific community on a case-by-case basis. With state of the art instrumentation, such as the standard configuration 3-lasers FACSAria located in BL2+-facility, 20-parameters 4-lasers FACSAria SORP and DIVA FACSVantage SE TurboSort™, the facility offers high speed cell sorting and complex analytical services, development of collaborative projects as well as consulting on design and development of new protocols and methods.
"FACSVantageDiVa is equipped with three lasers; A solid state ICyte laser producing 488nm blue line, one Spectra- Physics air cooled 633nm HeNe (red) laser, and a Coherent Innova 302 (krypton) Laser. Cell sorters are using DiVa software."
"FACSAria is a 3-laser, 11-parameter for flow sorting applications. The machine is equipped with a sample cooling system and has 2- and 4-way sorting options into 1, 3 and 15 ml tubes, as well as an Automated Cell Deposition Unit for microplate and slide sorting. The flow core designed Aria custom installation inside a BL2 + level biohazard containment cabinet with an internal Aerosol Management System and a separate remote system control workstation located outside of the BL2 + Lab provides ability to safely sort potentially suspect or known biohazardous samples. "
"A new 4-laser, 20-parameter FACSAria 2 SORP for flow sorting applications. The machine is equipped with a sample cooling system, 2- and 4-way sorting options into 1 and 15 ml tubes, as well as an Automated Cell Deposition Unit for microplate and slide sorting and Aerosol Management System. It also has a unique option of changing sorting needles (to adjust core size for nanoparticles sorting). Instrument available to investigators at 7/24 on-line schedule after initial training and passing a sorting test. "
"The 11-parameters FACSAria IIU is located at the BL2+ lab and is intended for infectious sorts and not-fixed human cells (with accordance of biosafety rules)."
"Four color, six parameter non-sorting bench top instruments capable of performing four color data acquisition and analysis. They are equipped with 488nm and 633 nm lasers."
The instrument "has 3 lasers (405 nm, 488 nm and 633 nm) and provides 8-colors, 10 parameters analytical capabilities."
"The Imagestream100 is a hybrid instrument combining the statistical power of flow cytometry and visual power of microscopy. The unique software program IDEAS allows it to perform robust, multiparameter quantitative analyses and generate brightfields, darkfields and fluorescence images of every cell. Graphics, statistics, images, and other data components can be easily copied to the Windows clipboard. The instrument implements the TDI (Time Delay Integration) System that greatly increases sensitivity of acquisition. The images' detection bands are fixed (470-500 nm; 400-470 nm; 500-560 nm; 560-595 nm; 595-660 nm; 660-735 nm). The system is compatible with a wide variety of fluorochromes and fluorescent proteins used in flow cytometry and microscopy."
"Provides institute investigators with state-of-the-art equipment along with human expertise to support cell sorting (separation), acquisition, and analysis of flow and imaging cytometric data."
"Offering comprehensive training, consultation for maximizing the efficiency of the experiments and collaborative development of new methods and applications."