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MGH Pathology: Flow, Image and Mass Cytometry Cores (Simches and CNY locations)

Director: Preffer, Fred, Ph.D.

Location: 13th Street, Building 149, Room 5.226, Charlestown Navy Yard, and 185 Cambridge Street, Room 3.434, Simches Building, Boston, MA 02114

Summary:

The core facilities at MGH Pathology provide flow cytometric cell sorting and analysis, image analysis and mass cytometry with the most sophisticated instruments currently available. There are two facility locations, one at MGH (Simches) and another at the Charlestown Navy Yard (CNY).

Flow Cytometry (Simches and CNY): The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies. We are able to sort unfixed specimens in our BSL2+ FACSAria Fusions, at both our Simches/Boston and Charlestown Navy Yard locations.

Multispectral Imaging Flow Cytometry (Simches): The Amnis technology combines flow with fluorescent and bright field image based analysis.

Mass Cytometry (CNY): Helios, which is a CyTOF (cytometry by Time-Of-Flight) system, permits single-cell analysis using stable heavy metal-tagged antibodies (~35) rather than fluorescent moieties.

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Last updated: 2019-02-06T14:30:28.816-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016