The core facilities at MGH Pathology provide flow cytometric cell sorting and analysis, image analysis and mass cytometry with the most sophisticated instruments currently available. There are two facility locations, one at MGH (Simches) and another at the Charlestown Navy Yard (CNY).
Flow Cytometry (Simches and CNY): The core is able to analyze and physically separate cells based upon features detectable with fluorescently labeled monoclonal antibodies. We are able to sort unfixed specimens in our BSL2+ FACSAria Fusions, at both our Simches/Boston and Charlestown Navy Yard locations.
Multispectral Imaging Flow Cytometry (Simches): The Amnis technology combines flow with fluorescent and bright field image based analysis.
Mass Cytometry (CNY): Helios, which is a CyTOF (cytometry by Time-Of-Flight) system, permits single-cell analysis using stable heavy metal-tagged antibodies (~35) rather than fluorescent moieties.
Mass cytometry uniquely combines time-of-flight mass spectrometry with Maxpar metal-labeling technology to enable breakthrough discovery and comprehensive functional profiling applications. Cellular targets are labeled with metal-tagged antibodies and detected and quantified by time-of-flight mass spectrometry. The high purity and choice of metal isotopes provide minimal background noise from signal overlap or endogenous cellular components. And 135 available detection channels ensure an ongoing ability to add more parameters, enabling you to fully study the functional complexity of biological systems at the single-cell level.
A four laser FACSAriaII cell sorter configured with 405 nm, 488 nm, 594 nm and 633 nm air cooled lasers.
Analytic four Laser Fortessa configured with 405 nm, 488 nm, 561 nm, and 633 nm air cooled lasers.
Analytic four Laser LSR II configured with 405 nm, 488 nm, 561 nm, and 633 nm air cooled lasers.
Configured with 373nm, 405nm, 488nm, 560nm, 592nm, 642nm lasers. This dual camera 12-channel instrument has 20x, 40x and 60x Magnification, EDF and 96-well plate capability.
Modified 5 Laser Digital Vantage SE cell sorter with 2 water-cooled argon, 2 krypton and one air cooled 633nm laser.
Analytic 8 Laser LSR
State-of-the-art multi-parametric flow cytometric analysis and cell sorting available at both Simches and Charlestown Navy Yard locations.