The Harvard Medical School EM Facility is a fee-for-service core facility open to all researchers. The Facility provides services and supervision in Transmission Electron Microscopy.
"For carbon and metal coating, rotary shadowing, glow-discharge."
"The JEOL can be used with film or DITABIS digital imaging plates. The digital imaging plates are loaded in the film holders and images are taken the same way as with traditional film, the imaging plates are then scanned and saved as TIFF files."
"For brightfield and immunoflourescence."
"Ultrathin frozen sections (60-90nm) are routinely used for immunogold localizations. (Tokayasu method)."
"Microtome for plastic sectioning."
"For easy trimming of resin embedded specimens."
"The Tecnai Spirit was installed in July 2005. The BioTWIN lens configuration is designed specifically for high-contrast imaging. The scope is equipped with an AMT 2k CCD camera."
"To be able to view a biological sample in the electron microscope it must first be stabilized or 'fixed' in a way that the ultrastructure of the cells or tissue remain as close to the living material as possible. The choice of fixative depends on the purpose of your study. It should be noted that, even though standard protocols can be sufficient for many purposes, finding the right fixation conditions is often a matter of trial and error. "
"The routine method for examining biological samples in TEM is to embed the material in plastic and cut ultrathin (~60-80nm) sections. The choice of resin depends on the type of sample and the purpose of your study. Listed below are some of the more commonly used ones. "
E.g., purified viruses and proteins.
Resin and frozen.