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Drosophila RNAi Screening Center (HMS)

Director: Mohr, Stephanie, Ph.D.

Location: New Research Building, Room 336, 77 Avenue Louis Pasteur, Boston, MA 02115

Summary:

The DRSC facilitates genome-wide and related Drosophila cell-based and in vivo screening at our state-of-the-art facility. We prove reagents, protocols and consultation throughout the screening process, including help with production of custom RNAi libraries; assay development, optimization and automation; data acquisition; data analysis and integration; and planning of follow-up assays.

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Instruments

  • Agilent Velocity Bravo liquid handling robot ( Liquid handler )

    Agilent Velocity Bravo liquid handling robot with 96-well format head for 96-to-96 or 96-to-384 micro-well format liquid handling tasks

  • Formulatrix Mantis liquid handling robot ( Liquid handler )

    Formulatrix Mantis liquid handling robot for addition of up to six reagents at a time to 384-well format micro-well plates, including in complex layouts (well-by-well addition, not column- or row-based addition)

  • GE IN Cell 6000 automated confocal imaging system ( Confocal microscope )

    High-content, high-throughput imaging at the DRSC/TRiP-FGR is supported by a GE IN Cell Analyzer 6000 Cell Imaging System with automation. Our IN Cell has 4x, 10x, 20x, and 60x lenses, and supports multi-color confocal or non-confocal fluorescence imaging as well as brightfield imaging. Please use the order/signup system or get in touch with the Director to learn about using this instrument for low- or high-throughput screens and other imaging projects.

  • LiCor Aerius fluorimeter ( Fluorometer )

    LiCor Aerius fluorimeter for ‘in cell Western’ and other total fluorescence readouts from 384-well format micro-well plates

  • Molecular Devices Paradigm luminometer and fluorimeter ( Fluorescence and luminescence assay detection system )

    Molecular Devices Paradigm luminometer and fluorimeter (“plate reader”) for total luminescence or fluorescence readouts from 384-well format micro-well plates

  • Perkin Elmer Multiprobe liquid handling robot ( Liquid handler )

    Perkin Elmer Multiprobe liquid handling robot for “cherry-picking” (individual selection) from 96-well format micro-well plates

Reagents