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Struhl Laboratory

Location: Department of Biological Chemistry and Molecular Pharmacology (BCMP), 240 Longwood Ave., Building C2, Rooms 315-325, Boston, MA 02115

Summary:

The molecular mechanisms of transcriptional regulation are highly conserved among eukaryotes. Transcriptional regulation in response to environmental and developmental cues is mediated by the combinatorial and synergistic action of specific DNA-binding activators and repressors on components of the general transcription machinery and chromatin modifying activities. Much of the work in this laboratory combines genetic, molecular, and genomic approaches available in yeast to address fundamental questions about transcriptional regulatory mechanisms in living cells. In addition, we are defining physiological targets of human transcriptional regulatory proteins and chromatin modifications on a whole-genome basis using a novel microarray approach.

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Organisms and Viruses

  • #699 (W303a) SST1+ (halo assay) ( Saccharomyces cerevisiae )

  • #957 (W303a) sst1- (halo assay) ( Saccharomyces cerevisiae )

  • 13B = SPY-ADE #1 ( Saccharomyces cerevisiae )

    MATa ade2::hisG his3::ADE2 leu2::PET56 met17-0 trp1-63 ura3-0. Based on FY1347 (S288C)/SPY2 #1.

  • 13E = KY320-4251 Vishy #6 ( Saccharomyces cerevisiae )

    4251 = min HIS3 prom. w/opt. GCN4 binding site. MATa ade2-101oc 4251p::HIS3 leu2::PET56 lys2-801am trp1- 1 ura3-52 GAL4+ GCN4 GCN5.

  • 13F = KY320-4251∆gcn5 (09a) ( Saccharomyces cerevisiae )

    MATa ade2-101oc 4251p:HIS3 leu2::PET56 lys2-801am trp1-∆1 ura3-52 GAL4+ GCN4 gcn5::URA3. 1XA-gcn5..URA3-YClac111 (SacI/SphI).

  • 13H = KY320-4251∆gcn5 (09a) ( Saccharomyces cerevisiae )

    Same as KY320-4251 but A1=gcn4-∆1. MATa ade2-101oc 4251p:HIS3 leu2::PET56 lys2-801am trp1-∆1 ura3-52 GAL4+ gcn4-∆1 GCN5.

  • 13I = KY320-4251-A1 ∆gcn5 (2a) ( Saccharomyces cerevisiae )

    MATa ade2-101oc 4251p:HIS3 leu2::PET56 lys2-801am trp1-∆1 ura3-52 GAL4+ gcn4-∆1 gcn5::URA3. 1XA-gcn5..URA3-YClac111 (SacI/SphI).

  • 14D = yMK839 ( Saccharomyces cerevisiae )

    MATa ADE2+ HIS3+ leu2- LYS2+ MET15+ trp1-ura3-.

  • 14E = yMK869 ( Saccharomyces cerevisiae )

    wt GCN4RE of HIS3 (ATGACTCTT) changed to 'GTCGACGTC', effectively destroying the GCN4 binding site. MATa ADE2+ his3-GCN4RE mutated leu2- LYS2+ MET15+ trp1-ura3-.

  • 14F = yMK886 ( Saccharomyces cerevisiae )

    as yMK869: wt GCN4RE of HIS3 (ATGACTCTT) changed to 'GTCGACGTC', effectively destroying the GCN4 binding site PLUS insertion of two optimal. GCN4 binding sites in the DED1 promoter (XhoI) ATGAGTCATtcgaATGACTCAT. MATa ADE2+ his3-GCN4RE mutated leu2- LYS2+ MET15+ trp1-ura3-DED1 w/GCN4RE.

  • 15B = SPY5-HIS3 (IV) ( Saccharomyces cerevisiae )

    HIS3 promoter integration w/19H SalI/SpeI: HIS3 prom ∆-683>-115 (only Tc/Tr). MATa ade2::hisG IV:±HIS3:TRP1 lys2::PET56 met17-∆0 trp1-∆63 ura3-∆0.

  • 19E = ySH120 (sc2884/ 38) gcn5 ( Saccharomyces cerevisiae )

    MAT? ADE2 ade8 Gcn4RE_(∆TC/∆TR):HIS3 leu2::PET56 lys2-∆202 trp1-∆161 ura3-52 ∆gcn5::URA3 gcn4-∆1 . replacement construct sc2884/∆38 deletes -106>-58 incl. TC & TR.

  • 19F = 8460::320 ( 93) gcn5 ( Saccharomyces cerevisiae )

    MAT? ade2-(?) Gcn4RE_(∆TC):HIS3 met? leu2-(?) ura3-52 ∆gcn5::URA3 gcn4-∆1 URA3::∆gcn5 . Based on KY329.

  • 1XA = KY1093 gcn5 ( Saccharomyces cerevisiae )

    MATa ade2-101oc Gal4:HIS3 (TATAAA) leu2-(?) lys2-801am met? trp1-∆1 ura3-52 URA3::∆gcn5 GCN4 . Based on KY117/KY114construct sc3640, i.e. Gal4:HIS3 (TATAAA).

  • 1XB = KY1101 gcn5 ( Saccharomyces cerevisiae )

    MATa ade2-101oc Gal4:HIS3 (TGTAAA) leu2-(?) lys2-801am met? trp1-∆1 ura3-52 URA3::∆gcn5 GCN4 . Based on KY117/KY114construct sc3660, i.e. Gal4:HIS3 (TGTAAA).

  • 1XC = KY1163 gcn5 ( Saccharomyces cerevisiae )

    MATa ade2-101oc Gal4:HIS3∆3'ofUAS>-35 (∆TC,∆TR) leu2-(?) lys2-801am met? trp1-∆1 ura3-52 URA3::∆gcn5 GCN4 . Based on KY117/KY114construct sc3304, i.e. Gal4:HIS3 (∆ 3' of UAS to -35: ∆TC & ∆TR).

  • 1XD = KY1164 gcn5 ( Saccharomyces cerevisiae )

    Based on KY117/KY114construct sc3304, i.e. Gal4:HIS3 (∆ 3' of UAS to -55: ∆TC but incl. TR). MATa ade2-101oc Gal4:HIS3∆3'ofUAS>-55 (∆TC,∆TR) leu2-(?) lys2-801am met? trp1-∆1 ura3-52 URA3::∆gcn5 GCN4 .

  • 1XE = KY320 A, 9000-8440 Vishy #97 gcn5 ( Saccharomyces cerevisiae )

    TC-TATA element of HIS3 replaced by TR-element (TATAAAG), plus optimal GCN4 binding site and ∆-96>-441. ∆gcn4!. MATa ade2-101oc opt. GCN4RE_2xTR:HIS3 leu2::PET56 lys2-801am MET-wt trp1-∆1 ura3-52 GAL4+ URA3::∆gcn5 gcn4-∆1 .

  • 1XF = KY320 A, 9305-8460 Vishy #132 gcn5 ( Saccharomyces cerevisiae )

    TC-TATA element of HIS3 replaced by CCTAAAC, TR-element (TATAAAG) replaced by TGTAAAG (no TBP binding), plus optimal GCN4 binding site and ∆-96>-441. ∆gcn4!. MATa ade2-101oc opt. GCN4RE_2xTGTAAAG:HIS3 leu2::PET56 lys2-801am MET-wt trp1-∆1 ura3-52 GAL4+ URA3::∆gcn5 gcn4-∆1 .

  • 24A = FT4 HIS3 A5 (no UAS)- 5 ( Saccharomyces cerevisiae )

    GCN5 was deleted with 1XA-gcn5::URA3 from M. MenciaBased on FT4 HIS3 A5 (dA::dT). MAT? no UAS::HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 24B = FT4 HIS3 A5 6231 (dA:dT)- 5 ( Saccharomyces cerevisiae )

    MAT? dA:dT::HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. cut with SacI/SphI this cnstruct replacesBased on FT4 HIS3 A5 (dA::dT).

  • 24C = FT4 HIS3 A5 7001 (Rap1)- 5 ( Saccharomyces cerevisiae )

    MAT? Rap1::HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. GCN5 -359>1321(Stop)Based on FT4 HIS3 A5 (Rap1).

  • 24D = FT4 HIS3 A5 7321 (Gcn4)- 5 ( Saccharomyces cerevisiae )

    with the URA3 marker (1-step)Based on FT4 HIS3 A5 (Gcn4). MAT? Gcn4:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 24E = FT4 HIS3 A5 7331 (Abf1)- 5 ( Saccharomyces cerevisiae )

    MAT? Abf1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Abf1).

  • 24F = FT4 HIS3 A5 7341 (Mcm1)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Mcm1). MAT? Mcm1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 24G = FT4 HIS3 A5 7351 (Ste12)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Ste12). MAT? Ste12:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 24H = FT4 HIS3 A5 7361 (Hap2-5)- 5 ( Saccharomyces cerevisiae )

    MAT? Hap2-5:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Hap2-5).

  • 24I = FT4 HIS3 A5 7371 (Reb1)- 5 ( Saccharomyces cerevisiae )

    MAT? Reb1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Reb1).

  • 24J = FT4 HIS3 A5 7381 (Leu3)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Leu3). MAT? Leu3:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25A = FT4 HIS3 A5 7391 (alpha1)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (alpha1). MAT? a1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25B = FT4 HIS3 A5 7401 (Swi6)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Swi6). MAT? Swi6:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25C = FT4 HIS3 A5 7411 (Swi4)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Swi4). MAT? Swi4:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25D = FT4 HIS3 A5 7421 (Adr1)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Adr1). MAT? Adr1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25E = FT4 HIS3 A5 7431 (Met4)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Met4). MAT? Met4:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25F = FT4 HIS3 A5 7441 (Hap1/CYC1)- 5 ( Saccharomyces cerevisiae )

    MAT? Hap1/CYC1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Hap1/CYC1).

  • 25G = FT4 HIS3 A5 7451 (Hap1/CYC7)- 5 ( Saccharomyces cerevisiae )

    MAT? Hap1/CYC7:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Hap1/CYC7).

  • 25H = FT4 HIS3 A5 7461 (Hsf1)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Hsf1). MAT? Hsf1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25I = FT4 HIS3 A5 7471 (ArgRII)- 5 Vishy #65 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (ArgRII). MAT? ArgRII:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 25J = FT4 HIS3 A5 7481 (Gal4)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Gal4). MAT? Gal4:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 26A = FT4 HIS3 A5 7491 (Swi5)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Swi5). MAT? Swi5:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 26B = FT4 HIS3 A5 7501 (Ppr1)- 5 ( Saccharomyces cerevisiae )

    MAT? Ppr1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Ppr1).

  • 26C = FT4 HIS3 A5 7511 (Put3)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Put3). MAT? Put3:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 26D = FT4 HIS3 A5 7521 (Bas1)- 5 ( Saccharomyces cerevisiae )

    MAT? Bas1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 (Bas1).

  • 26E = FT4 HIS3 A5 7531 (Bas2)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Bas2). MAT? Bas2:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 26F = FT4 HIS3 A5 8701 (Ace1)- 5 ( Saccharomyces cerevisiae )

    Based on FT4 HIS3 A5 (Ace1). MAT? Ace1:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3.

  • 26G = FT4 HIS3 A5 6231-7321 ((dA:dT)42-Gcn4)- 5 ( Saccharomyces cerevisiae )

    MAT? UASx:HIS3 leu2::PET56 trp1- 63 ura3-52 gcn5::URA3. Based on FT4 HIS3 A5 ((dA:dT)42-Gcn4).

  • 8460::320 (KY320 his3 TGTAA) ( Saccharomyces cerevisiae )

  • 8a = CC1 (toa1 ts/YCp22) ( Saccharomyces cerevisiae )

  • Ay43-2 = KY898 but acr1::URA3 ( Saccharomyces cerevisiae )

  • BJ2168 S. Saccharomyces Hansen, teleomorph (haploid) ( Saccharomyces cerevisiae )

    MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2. Homozygous prb1 mutations alter sporulation, homozygous pep4 mutations prevent itProteinase deficient (CH1)ATCC 208277.

  • BY105 ahc1 ::hisG ( Saccharomyces cerevisiae )

    ura3-52 trp1- 161 lys2- 202 gcn4- 1ahc1 ::hisG.

  • BY105 rad6 ::LEU2 ( Saccharomyces cerevisiae )

    ura3-52 trp1- 161 lys2- 202 gcn4- 1rad6 ::LEU2.

  • BY105 snf2 ::LEU2 ( Saccharomyces cerevisiae )

    ura3-52 trp1- 161 lys2- 202 gcn4- 1snf2 ::LEU2.

  • BY40 (CC1) TOA1+/YEp195 ( Saccharomyces cerevisiae )

  • BY4727 ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0.

  • BY4727 ADH2-9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87).

  • BY4727 BMH2 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87).

  • BY4727 ESF2 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87). Slow growth.

  • BY4727 IRC20 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87). Slow growth.

  • BY4727 JHD2 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87).

  • BY4727 YDR520C-9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87). Slow growth.

  • BY4727 Act1 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 Gcr2 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 LIA1 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87).

  • BY4727 Mer1 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 Myo1 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 OAF3 - 9myc ( Saccharomyces cerevisiae )

    BY4727 is MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0, tagged strain is TRP+ (tagged via PCR from pWZV87).

  • BY4727 Tye7 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 Yap2 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 Yap3 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4727 Yap7 - 9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • BY4730 Mat a Leu2 0 met15 0 ura3 0 ( Saccharomyces cerevisiae )

  • BY4741 + pZM419 (Ycplac33 Pgal1::3HA) ( Saccharomyces cerevisiae )

    MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741 Gal4 deletion (YPL248C) ( Saccharomyces cerevisiae )

    MATa his3 1 leu2 0 lys2 0 ura3 0.

  • BY4741 HMO1 deletion ( Saccharomyces cerevisiae )

    This is probably Dan Hall’s yDH544 - it was given to Jia Jing by Qian Wang, who got it from Joe Wade. .

  • BY4741, GAL1-454 (URA+) ( Saccharomyces cerevisiae )

    BY4741 containing 700bp GAL1/10 intergenic segment (in YIplac211) integrated at the 5’ end of YLR454w such that the GAL1-YLR454 joint is at the common ATG. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0YIplac211 is URA+.

  • BY4741, GAL1-454 (URA+), bre1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), bye1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), cdc73::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), chd1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), ctk1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), ctk2::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), ela1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), elp1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), elp3::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), elp4::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), fkh2::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), isw1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), leo1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), mft1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), ppr2::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), rad26::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), rrp6::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), rtf1::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), set2::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), spt4::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, GAL1-454 (URA+), thp2::KAN ( Saccharomyces cerevisiae )

    Grow on -URA medium. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1 ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, ctk1::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, elp1::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, elp4::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, leo1::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, rtf1::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, set2::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4741, HA-Hog1, thp2::KAN ( Saccharomyces cerevisiae )

    YPD. BY4741 is MATa his3 1 leu2 0 met 15 0 ura3 0.

  • BY4742 HSF1-3xHA ( Saccharomyces cerevisiae )

    Derivative of BY4742 and BY4741 with phenotype met+ and lys+.

  • BY4742 Med 15-3xHA (Gal11-3xHA) ( Saccharomyces cerevisiae )

    BY4742 containing C-terminally tagged protein expressed from natural promoter at normal chromosomal locus.

  • BY4742 Med 15-3xmyc (Gal11-3xmyc ) ( Saccharomyces cerevisiae )

    BY4742 containing C-terminally tagged protein expressed from natural promoter at normal chromosomal locus.

  • BY4742 Med 17-3xmyc (Srb4-3xmyc ) ( Saccharomyces cerevisiae )

    BY4742 containing C-terminally tagged protein expressed from natural promoter at normal chromosomal locus.

  • BY4742 Med 5-3xmyc (Nut1-3xmyc ) ( Saccharomyces cerevisiae )

    BY4742 containing C-terminally tagged protein expressed from natural promoter at normal chromosomal locus.

  • BY4742 FPR1 deletion (YNL135C) ( Saccharomyces cerevisiae )

    MATalpha his3 1 leu2 0 lys2 0 ura3 0.

  • BY4742 Gal4 deletion (YPL248C) ( Saccharomyces cerevisiae )

    MATalpha his3 1 leu2 0 lys2 0 ura3 0.

  • BY4742 set1::kan ( Saccharomyces cerevisiae )

  • BYdelta 2 ( Saccharomyces cerevisiae )

    Mat a, ura3-52, lys2-801, ade2-101, trp1- 1, GAL+, leu2::PET56. Carries YCp22 (CEN/TRP1) with yeast TFIID gene cloned as a 2.4 kb EcoRI-BamHI fragment). Derived from KY320.

  • BYdelta2 trp+ ( Saccharomyces cerevisiae )

  • BYdelta2 ts-1 ( Saccharomyces cerevisiae )

    Derived from BY 2. Contains chromosomal deletion of TBP.. See BY 2.

  • BYdelta2 ts-2 ( Saccharomyces cerevisiae )

    Derived from BY 2. Contains chromosomal deletion of TBP.. See BY 2.

  • BYdelta2 with 3.15 (T153I)/TRP1, mat a ( Saccharomyces cerevisiae )

    See BYdelta2.

  • BYdelta2 with 3.16 (F148H)/TRP1, mat a ( Saccharomyces cerevisiae )

    See BYdelta2.

  • BYdelta2 with 5.16 (F237D)/TRP1, mat a ( Saccharomyces cerevisiae )

    See BYdelta2.

  • BYdelta2 with 5.26 (E236P)/TRP1, mat a ( Saccharomyces cerevisiae )

    See BYdelta2. BY 2 with 5.26 (E236P)/TRP1, mat a.

  • BYdelta2/N2-1 (K138T, Y139A) ( Saccharomyces cerevisiae )

    See BYdelta2. Chromosomal TBP deletion covered by mutant N2-1 TBP on TRP1 vector. This strain grows slowly..

  • BYdelta40 (KY320, TOA1::ADE2) ( Saccharomyces cerevisiae )

    Constructed by S. Chou.

  • BYdelta40/plasmid pPK9 ( Saccharomyces cerevisiae )

    BYdelta40 is KY320, TOA1::ADE2pPK9 is CEN/TRP (based on YCplac22). Strain has genomic deletion of TOA1 (small subunit of TFIIA). N-terminally triple HA tagged TOA1 is supplied on pPK9 under natural TOA1 promoter.

  • CC1-U+ (BY40) ( Saccharomyces cerevisiae )

    KY320 toa1::ADE2 (TOA1/YEp195).

  • CC10-T+ = CC1 Ace1-his3 (TOA1/YCp22) ( Saccharomyces cerevisiae )

  • CC9-GF8, YCp33 ( Saccharomyces cerevisiae )

    CC1 Ppr1-his3 (gal1-FLAG-toa1 ts/YCp22 YCp33).

  • DD100 = DD89 + pD260 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD101 = DD89 + pD261 (UAS-PUT1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD102 = DD88 + pD263 (UAS-MLS1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD103 = DD88 + pD264 (UAS-CTR1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD104 = DD88 + pD265 (UAS-PUT1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD105 = DD88 + pD266 (UAS-MLS1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD106 = DD88 + pD267 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD107 = DD88 + pD268 (UAS-PUT1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD108 = DD88 + pD269 (UAS-MLS1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD109 = DD88 + pD270 (UAS-CTR1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD110 = DD88 + pD271 (UAS-PUT1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD111 = DD88 + pD272 (UAS-CYC1, TATA-MLS3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD112 = DD88 + pD273 (UAS-CTR1, TATA-MLS3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD113 = DD88 + pD274 (UAS-ERG3, TATA-MLS3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD114 = DD88 + pD275 (UAS-CYC1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD115 = DD88 + pD276 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD116 = DD88 + pD277 (UAS-ERG3, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD117 = DD88 + pD278 (UAS-CYC1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD118 = DD88 + pD279 (UAS-CTR1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD119 = DD88 + pD280 (UAS-ERG3, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD120 = DD89 + pD263 (UAS-MLS1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD121 = DD89 + pD264 (UAS-CTR1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD122 = DD89 + pD265 (UAS-PUT1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD123 = DD89 + pD266 (UAS-MLS1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD124 = DD89 + pD267 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD125 = DD89 + pD268 (UAS-PUT1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD126 = DD89 + pD269 (UAS-MLS1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD127 = DD89 + pD270 (UAS-CTR1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD128 = DD89 + pD271 (UAS-PUT1, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD129 = DD89 + pD272 (UAS-CYC1, TATA-MLS1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD130 = DD89 + pD273 (UAS-CTR1, TATA-MLS1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD131 = DD89 + pD274 (UAS-ERG3, TATA-MLS1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD132 = DD89 + pD275 (UAS-CYC1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD133 = DD89 + pD276 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD134 = DD89 + pD277 (UAS-ERG3, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD135 = DD89 + pD278 (UAS-CYC1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD136 = DD89 + pD279 (UAS-CTR1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD137 = DD89 + pD280 (UAS-ERG3, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD88 = MM101 + wt TBP (TRP1) ura- ura3-52 ( Saccharomyces cerevisiae )

  • DD89 = MM101 + N2-1 TBP (Trp1 ura- ura3-52) ( Saccharomyces cerevisiae )

  • DD90 = DD88 + pD256 (UAS-CYC1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD91 = DD88 + pD257 (UAS-CTK1, TATA-CTK1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD92 = DD88 + pD258 (UAS-ERG3, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD93 = DD88 + pD259 (UAS-ML51, TATA-ML51) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD94 = DD88 + pD260 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD95 = DD88 + pD261 (UAS-PUT1, TATA-PUT1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD96 = DD89 + pD256 (UAS-CYC1, TATA-CYC1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD97 = DD89 + pD257 (UAS-CTR1, TATA-CTR1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD98 = DD89 + pD258 (UAS-ERG3, TATA-ERG3) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DD99 = DD89 + pD259 (UAS-MLS1, TATA-MLS1) ( Saccharomyces cerevisiae )

    Plasmids pD256-pD280 are based on pRS306, a URA3 integration vector. All plasmids were integrated at the ura3-52 locus by linearizing with EcoRV. Basis for pD256-pD280 (UAS &TATA cassettes were moved around to give various combinations listed): EcoRV/------------UAS-TATA-GFP---------------/EcoRV.

  • DKY110 (leu+ 6AU+ lga- ura3-52) TFIIS+ ( Saccharomyces cerevisiae )

    DKY110 and DKY120 are, respectively, TFIIS+ and TFIIS- spores obtained after the sporulation of the GHY285xZ280 diploid. Unfortunately, I've never had a chance to test their basic phenotypes such as mating type, major auxotrophies etc. I tested only leu auxotrophy that stands for the HA-rpb3 and 6AU sensitivity that goes with TFIIS deletion. ura3-52 is obvious because it presented in both parents. Please see paper for the description of the lga phenotype.. .

  • DKY111 - ura3:pDK21 (WT-lacZ) ( Saccharomyces cerevisiae )

    DKY110 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY112 - ura3:pDK22 (UUC-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY110 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY113 - ura3:pDK23 (UC-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY110 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY114 - ura3:pDK24 (UU-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY110 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY115 - ura3:pDK25 (C-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY110 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY120 (leu+ 6AU- lga- ura3-52) TFIIS- ( Saccharomyces cerevisiae )

    DKY110 and DKY120 are, respectively, TFIIS+ and TFIIS- spores obtained after the sporulation of the GHY285xZ280 diploid. Unfortunately, I've never had a chance to test their basic phenotypes such as mating type, major auxotrophies etc. I tested only leu auxotrophy that stands for the HA-rpb3 and 6AU sensitivity that goes with TFIIS deletion. ura3-52 is obvious because it presented in both parents. Please see paper for the description of the lga phenotype..

  • DKY121 - ura3:pDK21 (WT-lacZ) ( Saccharomyces cerevisiae )

    DKY120 derivatives obtained by the integration ofthe plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ withdifferent versions of the Artificial Arrest site (see plasmids description).

  • DKY122 - ura3:pDK22 (UUC-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY120 derivatives obtained by the integration of the plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ with different versions of the Artificial Arrest site (see plasmids description).

  • DKY123 - ura3:pDK23 (UC-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY120 derivatives obtained by the integration of the plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ with different versions of the Artificial Arrest site (see plasmids description).

  • DKY124 - ura3:pDK24 (UU-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY120 derivatives obtained by the integration of the plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ with different versions of the Artificial Arrest site (see plasmids description).

  • DKY125 - ura3:pDK25 (C-ARTAR-lacZ) ( Saccharomyces cerevisiae )

    DKY120 derivatives obtained by the integration of the plasmid pDK2* into Ura3 locus. Plasmids pDK2* carry gal1:lacZ with different versions of the Artificial Arrest site (see plasmids description).

  • EAS16 = S. pombe with pES14 ( Schizosaccharomyces pombe )

    pES14 is S. pombe integrating vector pJK210 containing a 2789 bp PET56-HIS3 PCR product (-1209 to +1580 relative to the HIS3 ATG). pJK210 is URA+. Tube is labeled yES16.

  • EHEM12 Eaf3 - HA, Esa1- myc ( Saccharomyces cerevisiae )

  • EHRM3 Eaf3 - HA, Rpd3- myc ( Saccharomyces cerevisiae )

  • FG2156 S. pombe (h- atf1-12 myc URA4+) ( Schizosaccharomyces pombe )

  • Fhl1 (haploid derivative of BY4743) ( Saccharomyces cerevisiae )

    Unknown.

  • FP102 ( Saccharomyces cerevisiae )

  • FP104 ( Saccharomyces cerevisiae )

  • FP110 ( Saccharomyces cerevisiae )

  • FP112 ( Saccharomyces cerevisiae )

  • FP132 FY3 TFIIBts ( Saccharomyces cerevisiae )

  • FP134 (S288C a ura3-52 trp1 161 IIBts) ( Saccharomyces cerevisiae )

  • FP135 (S288C ura3-52 trp1 161 IIBts) ( Saccharomyces cerevisiae )

  • FP136 (S288C a ura3-52 lys2 202 IIBts) ( Saccharomyces cerevisiae )

  • FP137 (S288C ura3-52 lys2 202 IIBts) ( Saccharomyces cerevisiae )

  • FP155 ( Saccharomyces cerevisiae )

  • FP157 ( Saccharomyces cerevisiae )

  • FP158 ( Saccharomyces cerevisiae )

  • FP159 ( Saccharomyces cerevisiae )

  • FT4 ( Saccharomyces cerevisiae )

    Mat a, otherwise isogenic to FT5. Derived from S288C. Mat a ura3-52 trp1 63 his3 200 leu2::PET56.

  • FT4 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • FT4 sin3::HIS3 ( Saccharomyces cerevisiae )

  • FT4 9A 3HA-Set1 ( Saccharomyces cerevisiae )

    Mata, ura3-52 trp1-D63 his3-D200 leu2::PET56 3xHA-SET1. Set1 tagged at N-terminus.

  • FT4 9A 3HA-Set1 cdc73::Kan ( Saccharomyces cerevisiae )

    Mata, ura3-52 trp1-D63 his3-D200 leu2::PET56 3xHA-SET1. Set1 tagged at N-terminus.

  • FT4 9A 3HA-Set1 paf1::Kan ( Saccharomyces cerevisiae )

    Mata, ura3-52 trp1-D63 his3-D200 leu2::PET56 3xHA-SET1. Set1 tagged at N-terminus.

  • FT4 9A 3HA-Set1 rtf1::Kan ( Saccharomyces cerevisiae )

    Mata, ura3-52 trp1-D63 his3-D200 leu2::PET56 3xHA-SET1. Set1 tagged at N-terminus.

  • FT4 HA3-Set1 ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56.

  • FT4 HA3-Set1 [cdc73::kan] ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 cdc73::kan.

  • FT4 HA3-Set1 [paf1::kan] ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 paf1::kan.

  • FT4 HA3-Set1 [rad6::kan] ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 rad6::kan.

  • FT4 HA3-Set1 [rtf1::kan] ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 rtf1::kan.

  • FT4 HIS3 A5 7461-Hsf ( Saccharomyces cerevisiae )

    See FT4. Contains a heat-shock driven his3 promoter.

  • FT4 Rad6-9myc [rtf1::kan] ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 rad6::kan.

  • FT4 Rad6-9myc::TRP ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 rad6::kan.

  • FT4 STH1 - 9 myc ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 . Protein tagged at C-terminus with 9myc::TRP1 cassette (Cosma et al.. 1999. Cell 97:299-311).

  • FT4 X FT5 ( Saccharomyces cerevisiae )

    MATa /alpha his3-D200 leu2::PET56 trp1-D63 ura 3-52(2n his3-D200 leu2::PET56 trp1-D63 ura 3-52).

  • FT4 YCplac22 ( Saccharomyces cerevisiae )

  • FT4/FT5 diploid ( Saccharomyces cerevisiae )

  • FT5 ( Saccharomyces cerevisiae )

    Mat , otherwise isogenic to FT4. Derived from S288C. Mat ura3-52 trp1 63 his3 200 leu2::PET56.

  • FT5 gcn5::LEU2 ( Saccharomyces cerevisiae )

  • FT5 gcn5::LEU2 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • FT5 gcn5::LEU2 sin3::HIS3 ( Saccharomyces cerevisiae )

  • FT5 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • FT5 sin3::HIS3 ( Saccharomyces cerevisiae )

  • FT5 sin4::HIS3 ( Saccharomyces cerevisiae )

  • FT5 ume6::LEU2 ( Saccharomyces cerevisiae )

  • FT5 HDA1-HA3 (YIplac128 - LEU) ( Saccharomyces cerevisiae )

  • FT5 HOS1-HA3 (YIplac128 - LEU) ( Saccharomyces cerevisiae )

  • FT5 HOS2-HA3 (YIplac128 - LEU) ( Saccharomyces cerevisiae )

  • FT5 HOS3-HA3 (YIplac128 - LEU) ( Saccharomyces cerevisiae )

  • FT5 RPD3-HA3 (YIplac128 - LEU) ( Saccharomyces cerevisiae )

  • FY104 ( Saccharomyces cerevisiae )

    From Fred Winston.

  • FY1291 ( Saccharomyces cerevisiae )

    MATa ura3-52 leu2 1 lys2-173R2 trp1 63 arg4-12 spt20 200::ARG4.

  • FY1346 ( Saccharomyces cerevisiae )

    MAT alpha ade2 ::hisG his3 200 leu2 0 met 15 0 trp1 63 ura3 0.

  • FY1346 X SPY5 ( Saccharomyces cerevisiae )

    MATa /alpha ade2::hisG his3-D200 leu2-D0 LYS2 met17-D0 trp1-D63 ura3-D0(2n ade2::hisG his3::ADE2 LEU2 lys2::PET56 met17-D0 trp1-D63 ura3-D0).

  • FY1351 (MATa, leu2 0, lys2 0, ura3 0) ( Saccharomyces cerevisiae )

    MATa, leu2 0, lys2 0, ura3 0 (isogenic to S288C).

  • FY406 ( Saccharomyces cerevisiae )

    FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pSAB6 (URA3, CEN, ARS, HTA1-HTB1).

  • FY406 FLAG-H2B dot1::kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Rtf1:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp10:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp11:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp12:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp13:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp14:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp15:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp16:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp1::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp2::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp3::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp4::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp5::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp6::Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp7:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp8:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ Ubp9:Kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B HIS+ URA- ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200. Transformed with modified pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 FLAG-H2B rad6::kan ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • FY406 K123R (H2B) HIS+, URA- Clone 1 ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200. Transformed with modified pJH23 (FB1251) HIS3 AMP CEN HTA1-HTB2 with K123R mutation.

  • FY406 K123R (H2B) HIS+, URA- Clone 2 ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, FB1251 (pJH23 = HIS3 AMP CEN HTA1-HTB2).

  • FY406 WT H2B HIS+, URA- ( Saccharomyces cerevisiae )

    FB1251 (pJH23) made by cloning 2.6 kb Bam-SacII fragment containing HTA1-HTB2 from pCC60 (FB1247) into Bam-SacII of pRS313.. FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200, lys-128deltapJH23 (FB1251) HIS3 AMP CEN HTA1-HTB2.

  • FY56 HA-Rbp3 GAL1-YLR454 ( Saccharomyces cerevisiae )

    Parent strain was provided by Fred Winston. MAT HA-Rbp3 GAL1-YLR454 his4-912 lys2-128 ura3-52.

  • FY56 HA-Rbp3 YLR454 ( Saccharomyces cerevisiae )

    MAT HA-Rbp3 his4-912 lys2-128 ura3-52. Parent strain was provided by Fred Winston.

  • GHY285 (hisG replacement of ppr2) ( Saccharomyces cerevisiae )

    GHY285 is the TFIIS-minus strain from Grant Hartzog . This strain was obtained by hisG replacement of ppr2 gene. This replacement was accomplished by the transforming the basic Fred Winston strain FY653 (mat alpha his4-912-delta lys2-128-delta leu2-delta-1 ura3-52) with the ppr2-hisG plasmid pJD3 constructed in Caroline Kane's lab (UC-Berkley).In order to cross this strain with Z280, I looped out the hisG-Ura3 by growing GHY285 at the FOA plate and used the revertants for the cross. Mat alpha his4-912-delta lys2-128-delta leu2-delta-1 ura3-52ppr2::hisG-Ura3.

  • IIB shutoff ( Saccharomyces cerevisiae )

    MATa ade2-101 leu2::PET56 trp1-D1 ura 3-52 Cu inducible ROX1/UBR1 TFIIB shut-off (URA+).

  • JD41 (HIS3 allele Sc4251 integrated into FT5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56. SEE INFO SHEET FOR MORE DETAILS(old number JDY4251).

  • JD41 (HIS3 allele Sc4251 integrated into FT5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56. SEE INFO SHEET FOR MORE DETAILS(old number JDY4251).

  • JD8702 (HIS3 allele HIS3A5-8701 integrated into FT5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56. SEE INFO SHEET FOR MORE DETAILS.

  • JDY1 (WT) ( Saccharomyces cerevisiae )

    LYS2 locus: LYS2. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: his3d200 SEE INFO SHEET FOR MORE DETAILS.

  • JDY100 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD130 alpha2MCM1-Gcn4LYS2 locus: int pJD21 Gcn4.

  • JDY101 ssn6::LEU2 (sick & clumpy) ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD130 alpha2MCM1-Gcn4LYS2 locus: int pJD21 Gcn4.

  • JDY102 tup1::URA3 (sick & clumpy) ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD130 alpha2MCM1-Gcn4LYS2 locus: int pJD21 Gcn4.

  • JDY11 (WT) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD11 INO1-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY110 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 6231 dA:dTLYS2 locus: int pJD80 dA:dT. SEE INFO SHEET FOR MORE DETAILS.

  • JDY111 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD60 IME2-dA:dTLYS2 locus: int pJD80 dA:dT. SEE INFO SHEET FOR MORE DETAILS.

  • JDY112 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD60 IME2-dA:dTLYS2 locus: int pJD80 dA:dT. SEE INFO SHEET FOR MORE DETAILS.

  • JDY113 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD60 IME2-dA:dTLYS2 locus: int pJD80 dA:dT. SEE INFO SHEET FOR MORE DETAILS.

  • JDY114 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD60 IME2-dA:dTLYS2 locus: int pJD80 dA:dT. SEE INFO SHEET FOR MORE DETAILS.

  • JDY12 (rdp3 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD11 INO1-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY120 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7001 Rap1LYS2 locus: int pJD81 Rap1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY121 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD61 IME2-Rap1LYS2 locus: int pJD81 Rap1.

  • JDY122 rpd3 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD61 IME2-Rap1LYS2 locus: int pJD81 Rap1.

  • JDY123 sin3 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD61 IME2-Rap1LYS2 locus: int pJD81 Rap1.

  • JDY124 ume6 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD61 IME2-Rap1LYS2 locus: int pJD81 Rap1.

  • JDY125 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY122.

  • JDY126 rpd3 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY122.

  • JDY13 (sin3 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD11 INO1-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY130 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7331 Abf1LYS2 locus: int pJD83 Abf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY131 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD63 IME2-Abf1LYS2 locus: int pJD83 Abf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY132 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD63 IME2-Abf1LYS2 locus: int pJD83 Abf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY133 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD63 IME2-Abf1LYS2 locus: int pJD83 Abf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY134 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD63 IME2-Abf1LYS2 locus: int pJD83 Abf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY135 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY131. SEE INFO SHEET FOR MORE DETAILS.

  • JDY136 rpd3 ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY131. SEE INFO SHEET FOR MORE DETAILS.

  • JDY14 (UME6 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD11 INO1-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY140 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7381 Leu3LYS2 locus: int pJD95 Leu3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY141 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD65 IME2-Leu3LYS2 locus: int pJD95 Leu3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY142 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD65 IME2-Leu3LYS2 locus: int pJD95 Leu3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY143 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD65 IME2-Leu3LYS2 locus: int pJD95 Leu3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY144 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD65 IME2-Leu3LYS2 locus: int pJD95 Leu3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY150 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7511 Put3LYS2 locus: int pJD99 Put3.

  • JDY151 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD70 IME2-Put3LYS2 locus: int pJD99 Put3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY152 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD70 IME2-Put3LYS2 locus: int pJD99 Put3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY153 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD70 IME2-Put3LYS2 locus: int pJD99 Put3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY154 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD70 IME2-Put3LYS2 locus: int pJD99 Put3. SEE INFO SHEET FOR MORE DETAILS.

  • JDY160 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7461 Hsf1LYS2 locus: int pJD96 Hsf1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY161 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD67 IME2-Hsf1LYS2 locus: int pJD96 Hsf1.

  • JDY162 rpd3 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD67 IME2-Hsf1LYS2 locus: int pJD96 Hsf1.

  • JDY163 sin3 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD67 IME2-Hsf1LYS2 locus: int pJD96 Hsf1.

  • JDY164 ume6 KO ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD67 IME2-Hsf1LYS2 locus: int pJD96 Hsf1.

  • JDY170 WT (formerly JDY7481) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 7481 Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY171 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY172 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY173 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY174 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY175 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY172. SEE INFO SHEET FOR MORE DETAILS.

  • JDY176 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY172. SEE INFO SHEET FOR MORE DETAILS.

  • JDY180 WT (formerly JDY8701) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int 8701 Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY181 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY182 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY183 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY184 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY185 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into pJD181. SEE INFO SHEET FOR MORE DETAILS.

  • JDY186 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into pJD181. SEE INFO SHEET FOR MORE DETAILS.

  • JDY187 ace1 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY188 ace1; rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD73 IME2-Ace1LYS2 locus: int pJD93 Ace1. SEE INFO SHEET FOR MORE DETAILS.

  • JDY191 gcn5 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY192 gcn5; rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY193 swi2 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY194 swi2; rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD68 IME2-Gal4LYS2 locus: int pJD97 Gal4. SEE INFO SHEET FOR MORE DETAILS.

  • JDY2 (rpd3 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: his3d200 LYS2 locus: LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY201 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Ada2-myc18 integrated into JDY51 (Gcn4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY202 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Ada2-myc18 integrated into JDY51 (Gcn4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY203 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Swi2-myc18 integrated into JDY51 (Gcn4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY204 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Swi2-myc18 integrated into JDY51 (Gcn4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY21 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD12 IME2-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY211 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Ada2-myc18 integrated into JDY171 (Gal4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY212 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Ada2-myc18 integrated into JDY171 (Gal4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY213 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Swi2-myc18 integrated into JDY171 (Gal4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY214 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Swi2-myc18 integrated into JDY171 (Gal4-HIS3). SEE INFO SHEET FOR MORE DETAILS.

  • JDY22 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD12 IME2-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY220 (= yLK29A rpd3) ( Saccharomyces cerevisiae )

    yLK29 = W303-1a + 3xHA TBP TFIIB-Myc9-KLTRP1.

  • JDY23 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD12 IME2-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY24 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD12 IME2-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY3 (sin3 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: his3d200 LYS2 locus: LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY301 ace1 KO pACE1 ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pACE1.

  • JDY302 ace1rpd3 KO (pACE1) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pACE1.

  • JDY303 ace1 KO (pAC-Hsf1) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Hsf1.

  • JDY304 ace1rpd3 KO (pAC-Hsf1) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Hsf1.

  • JDY305 ace1 KO (pAC-Gcn4) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gcn4.

  • JDY306 ace1rpd3 KO (pAC-Gcn4) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gcn4.

  • JDY307 ace1 KO (pAC-Gal4) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gal4.

  • JDY308 ace1rpd3 KO (pAC-Gal4) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gal4.

  • JDY309 ace1 KO (pAC-Gal11) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gal11.

  • JDY31 WT ( Saccharomyces cerevisiae )

    SEE INFO SHEET FOR MORE DETAILS. FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD13 LexA-CYC1/HIS3LYS2 locus: int pJD17 lys2::CYC1/LEU2.

  • JDY310 ace1rpd3 KO (pAC-Gal11) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Gal11.

  • JDY311 ace1 KO (pAC-TBP) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TBP.

  • JDY312 ace1rpd3 KO (pAC-TBP) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TBP.

  • JDY313 ace1 KO (pAC-TAF17) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF17.

  • JDY314 ace1rpd3 KO (pAC-TAF17) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF17.

  • JDY315 ace1 KO (pAC-TAF19) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF19.

  • JDY316 ace1rpd3 KO (pAC-TAF19) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF19.

  • JDY317 ace1 KO (pAC-TAF23) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF23.

  • JDY318 ace1rpd3 KO (pAC-TAF23) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-TAF23.

  • JDY319 ace1 KO (pAC-Srb5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Srb5.

  • JDY320 ace1rpd3 KO (pAC-Srb5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56Carries plasmid based on pRS305 (URA5): pAC-Srb5.

  • JDY4 (ume6 KO) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: his3d200 LYS2 locus: LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY41 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD21 X-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY42 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD21 X-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY43 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD21 X-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY44 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD21 X-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY51 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY52 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY53 sin3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY54 ume6 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY61 gnc5 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY62 gnc5; rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY63 swi2 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY64 swi2; rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD30 -10IME2-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY65 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY52. SEE INFO SHEET FOR MORE DETAILS.

  • JDY66 rpd3 KO ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET563-HA-TBP integrated into JDY52. SEE INFO SHEET FOR MORE DETAILS.

  • JDY70 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD34 -100IME2-Sc4251LYS2 locus: int pJD54 -100X/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY71 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD35 -200IME2-Sc4251LYS2 locus: int pJD55 -200X/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY72 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD37 -400IME2-Sc4251LYS2 locus: int pJD57 -400X/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY73 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD39 -800IME2-Sc4251LYS2 locus: int pJD59 -800X/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY7482 (HIS3 allele HIS3A5-7481 integrated into FT5) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56. SEE INFO SHEET FOR MORE DETAILS.

  • JDY8 (WT) ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: his3d200 LYS2 locus: int pJD17 lys2::CYC1/LEU2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY90 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD48 -10LexAop-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JDY92 WT ( Saccharomyces cerevisiae )

    FT5 a, ura3-52, trp1D63, leu2::PET56HIS3 locus: int pJD49 -100LexAop-Sc4251LYS2 locus: int pJD52 X-Sc4251/LYS2. SEE INFO SHEET FOR MORE DETAILS.

  • JGY100 ( Saccharomyces cerevisiae )

    MATa, ura3-42, trp1- 1, his3::SUC2-HIS3, lys2-801am, ade2-101oc, leu2::leu2-PET56, spt15 , YCplac33-TBP.

  • JHY89 (histone H3 mutations: S10, S28A) ( Saccharomyces cerevisiae )

  • JRY1 = Esa1 shutoff in ZMY117 (1step with 25A) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56. Confirmed by PCR with 2918/2919, HA-ESA1 degradation following copper addition, death on copper .

  • JRY1 = Esa1 shutoff in ZMY117 (2-step with 25G) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56. Confirmed by PCR with 2918/2919, HA-ESA1 degradation following copper addition, death on copper .

  • JRY1 YCplac22 ESA1 ( Saccharomyces cerevisiae )

    TRP1.

  • JRY10 shuttle strain ( Saccharomyces cerevisiae )

    Parent strain JRY5 (FY1346 X SPY5)Confirm by PCR with 3582/3378, Grow on CAA-U, Cannot grow on SCD FOA, Halo assay for mating type. . MATa ade2::hisG his3::ade2 LYS? met17- 0 trp1- 63 ura3-52 esa1::hisG (ESA1 null with YCplac33-ESA1 covering plasmid from JRY3 sporulation).

  • JRY11 YCplac22-HA-ESA1 shuttle ( Saccharomyces cerevisiae )

    MATa his3:: leu2::PET56 trp1- 63 ura3-52 esa1::hisG (ESA1 null with YCplac22-HA-ESA1 shuttled in). Parent strain JRY8 (FT4 X FT5)Confirm by growth on FOA, doesn't grow on CAA-U, does grow on CAA-T.

  • JRY14 = 3xHA-ESA1 RPSIIB Rap1 ( Saccharomyces cerevisiae )

    MATa, leu2 0, lys2 0, ura3 0 (isogenic to S288C)Mutant RPSIIB promoter on YCplac33 (URA3). Strain contains both WT and mutant promoters for RPS11BGROW ON CAA -URA.

  • JRY15 RPL19B-DRAP1 integrated ( Saccharomyces cerevisiae )

    Parent strain JRY4 (FY1351)PCR with 4694/4695, Two bands for RPS11B ChIP. MATa leu2-D0 lys2-D0 ura3-D0 (3xHA-ESA1 replacing Genomic copy, RPS11B-DRAP1 integrated (URA3) at genomic locus with WT copy remaining).

  • JRY16 RPL19B-DRAP1 CEN ( Saccharomyces cerevisiae )

    Parent strain JRY4 (FY1351)I never used this strain as the primers for ChIP were not good - JR. MATa leu2-D0 lys2-D0 ura3-D0 (3xHA-ESA1 replacing Genomic copy plus yCplac33-RPL19B-DRAP1).

  • JRY17 RPL19B-DRAP1 CEN ( Saccharomyces cerevisiae )

    MATa leu2-D0 lys2-D0 ura3-D0 (3xHA-ESA1 replacing Genomic copy, RPL19B-DRAP1 at genomic locus with WT copy remaining). Parent strain JRY4 (FY1351)PCR with 4692/4693, I never used this strain for ChIP - JR.

  • JRY18 HA-ESA1 ( Saccharomyces cerevisiae )

    Parent strain ResGen BY4741Confirm by PCR with 3425/3324, 6101/5818, 6100/6139, expression of HA-ESA1. MATa his3-D1 leu2-D0 met 15-D0 ura3-D0 esa1::HA-ESA1 3xHA-ESA1 replacing Genomic copy (2-step with yIplac211 HA-ESA1 1-444bp).

  • JRY19 HA-ESA1 eaf3::KAN ( Saccharomyces cerevisiae )

    MATa his3-D1 leu2-D0 met 15-D0 ura3-D0 eaf3::KAN 3xHA-ESA1 replacing Genomic copy (2-step with yIplac211 HA-ESA1 1-444bp). Parent strain ResGen 7143 ( EAF3)Confirm by PCR with 3425/3324, 6101/5818, 6100/6139, expression of HA-ESA1, growth on G418.

  • JRY20 HA-ESA1 ( Saccharomyces cerevisiae )

    Parent strain JRY8 (FT4 X FT5)Confirm by growth on FOA, doesn't grow on CAA-U, does grow on CAA-T. MATa his3-D200 leu2::PET56 trp1-D63 ura3-52 es1::hisG 1 (ESA1 null with yCplac22-ESA1 shuttled in).

  • JRY21 ESA1 (ts) ( Saccharomyces cerevisiae )

    MATa his3-D200 leu2::PET56 trp1-D63 ura3-52 esa1::hisG (ESA1 null with yCplac22-ESA1-D414 (ts) shuttled in). Parent strain JRY8 (FT4 X FT5)Growth on FOA, doesn't grow on CAA-U, does grow on CAA-T, ts at 37, slow growth at 30.

  • JRY22 HA-ESA1 (ts) ( Saccharomyces cerevisiae )

    Parent strain JRY8 (FT4 X FT5)Growth on FOA, doesn't grow on CAA-U, does grow on CAA-T, ts at 37, slow growth at 30. MATa his3-D200 leu2::PET56 trp1-D63 ura3-52 esa1::hisG (ESA1 null with yCplac22-ESA1-D414 (ts) shuttled in).

  • JRY23 RPS11B-DRAP1, loop out ( Saccharomyces cerevisiae )

    MATa leu2-D0 lys2D0 ura3-52 esa1::hisG 3xHA-ESA1 replacing Genomic copy, RPS11B-DRAP1 at genomic locus (looped out so that no WT RPS11B allele). Parent strain JRY15 (FY1351)Growth on FOA, PCR with 4694/4695.

  • JRY24 HA-ESA1 ( Saccharomyces cerevisiae )

    MATa ade2::hisG his3::ADE2 leu2::PET56 met17-D0 trp1-D63 ura3-52 esa1::HA-ESA13xHA-ESA1 replacing Genomic copy (2-step with yIplac211 HA-ESA1 1-444bp). Parent strain SPY-ADEConfirm by PCR with 3425/3324, expression of HA-ESA1.

  • JRY3 = Esa1::hisG (2-step with pBS ESA1-HisG-Ura3) ( Saccharomyces cerevisiae )

    Mat a/alpha, his3-D200 leu2::PET56 trp1-D63 ura3-52 esa1::hisG. Parent strain FT4 x FT5Confirm by PCR with 3582/3378, tetrad analysis essential.

  • JRY4 = FY1351 3xHA-ESA1 ( Saccharomyces cerevisiae )

    MATa, leu2 0, lys2 0, ura3 0 (isogenic to S288C)3xHA-ESA1 replacing genomic copy (2-step with YIplac211 HA-ESA1 1-444bp). Confirm by PCR with 3425/3324, expression of HA-ESA1.

  • JRY5 = ESA1::hisG (2-step with pBS ESA1-HisG-Ura3) ( Saccharomyces cerevisiae )

    Parent strains FY1346 X SPY5Confirm by PCR with 3582/3378, tetrad analysis essential. MATa/alpha ade2::hisG his3- 200 leu2- 0 LYS2 met17- 0 trp1- 63 ura3- 0 esa1::hisG (2n his3::ade2 LEU2 lys2::PET56).

  • JRY5 YCplac33 ESA1 ( Saccharomyces cerevisiae )

    URA3.

  • JRY7 shuttle strain ( Saccharomyces cerevisiae )

    Parent strain JRY3 (FT4 X FT5)Confirm by PCR with 3582/3378, Grow on CAA-U, Cannot grow on SCD FOA, Halo assay for mating type. . MATalpha his3- 200 leu2::Pet56 trp1- 63 ura3-52 esa1::hisG (ESA1 null with YCplac33-ESA1 covering plasmid from JRY3 sporulation).

  • JRY8 shuttle strain ( Saccharomyces cerevisiae )

    Parent strain JRY3 (FT4 X FT5)Confirm by PCR with 3582/3378, Grow on CAA-U, Cannot grow on SCD FOA, Halo assay for mating type. . MATa his3- 200 leu2::Pet56 trp1- 63 ura3-52 esa1::hisG (ESA1 null with YCplac33-ESA1 covering plasmid from JRY3 sporulation).

  • JRY9 shuttle strain ( Saccharomyces cerevisiae )

    Parent strain JRY5 (FY1346 X SPY5)Confirm by PCR with 3582/3378, Grow on CAA-U, Cannot grow on SCD FOA, Halo assay for mating type. . MATalpha ade2::hisG his3::ade2 leu2:: 60 LYS? met17- 0 trp1- 63 ura3-52 esa1::hisG (ESA1 null with YCplac33-ESA1 covering plasmid from JRY3 sporulation).

  • JTW1 = BY4727 Fhl1-9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+NOTE: This strain was remade. As of 11/4/05 it's been checked by PCR but not by ChIP.

  • JTW2 (BY4727 Ifh1-9myc) + pDH053 ( Saccharomyces cerevisiae )

    TRP+. BY4727 = MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0pDH053 = YCp111 (CEN/LEU) Eft2 prom - 3HA - Gal4(1-147)-CYC1.

  • JTW2 (BY4727 Ifh1-9myc) + pDH053 FHA domain ( Saccharomyces cerevisiae )

    TRP+FHA domain of Fhl1 fused to Gal4 DBD. BY4727 = MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0pDH053 = YCp111 (CEN/LEU) Eft2 prom - 3HA - Gal4(1-147)-CYC1.

  • JTW2 (BY4727 Ifh1-9myc) + pDH053 Gcr1 ( Saccharomyces cerevisiae )

    TRP+Gcr1 fused to Gal4 DBD. BY4727 = MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0pDH053 = YCp111 (CEN/LEU) Eft2 prom - 3HA - Gal4(1-147)-CYC1.

  • JTW2 (BY4727 Ifh1-9myc) + YCplac111 (CEN/LEU) ( Saccharomyces cerevisiae )

    TRP+. BY4727 = MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0.

  • JTW2 = BY4727 Ifh1-9myc ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+.

  • JTW3 (See description) Gal induction of ifh1 ( Saccharomyces cerevisiae )

    Haploid derivative of BY4743 ifh1 with covering plasmid pJZW1 (pZM419-ifh1-3HA, derived from YCplac33 CEN/URA).

  • JTW3b (See comments) Gal induction of fhl1 ( Saccharomyces cerevisiae )

    Haploid derivative of BY4743 fhl1 with covering plasmid pJZW1 (pZM419-fhl1-3HA, derived from YCplac33 CEN/URA).

  • JTW4 = BY4727 + Fhl1-9myc (TRP1) + Ifh1-TAP (URA3) ( Saccharomyces cerevisiae )

    BY4727 = MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0.

  • JTW5 = BY4727 Fhl1-9myc Ifh1-TAP HMO1 ( Saccharomyces cerevisiae )

    MATalpha, his3 200, leu2 0, lys2 0, met15 0, trp1 63, ura3 0. TRP+NOTE: This strain was remade. As of 11/4/05 it's been checked by PCR but not by ChIP.

  • KY 516 (KS55 ope1-1) ( Saccharomyces cerevisiae )

    KY55 = KY162 (YR 21-Sc2782 TRP1+ his3- 13)KY162 = Mat a, ura3-52, trp1-289, his3- 200, can1. Crunchy mutantHIS+ revertant.

  • KY114a ( Saccharomyces cerevisiae )

  • KY1151 = KY320 with Sc3647 ( Saccharomyces cerevisiae )

    Paper has details of constructsSC3647 has Gal promoter.

  • KY1152 = KY320 with Sc3647 and T7 RNAP ( Saccharomyces cerevisiae )

    Paper has details of constructsSC3647 has Gal promoter.

  • KY1229 = KY320 with Sc3680 ( Saccharomyces cerevisiae )

    Paper has details of constructsSC3680 has no ded1 element.

  • KY1232 = KY320 with Sc3680 and T7 RNAP ( Saccharomyces cerevisiae )

    URA+. Paper has details of constructsSC3680 has no ded1 element.

  • KY1239 = KY320 with Sc3684 ( Saccharomyces cerevisiae )

    The details of the construct design are described in the publication.
    SC3684 has Ded1 promoter.

  • KY1242 = KY320 with Sc3684 and T7 RNAP ( Saccharomyces cerevisiae )

    Paper has details of constructsSC3684 has Ded1 promoter. URA+.

  • KY2031 or Z25 ( Saccharomyces cerevisiae )

    RPS13:TRP allele of KY2002. This is one of only four C. Klein strains available. Most of his stocks were either missing or could not be resuscitated..

  • KY30 ura3-52 trp1-289 his3 1 ( Saccharomyces cerevisiae )

  • KY320 ( Saccharomyces cerevisiae )

    Mat ura3-52, lys2-801, ade2-101, trp1- 1, his3 200, GAL+, leu2::PET56. Similar to KY117, constructed by replacing the leu2 locus of KY117 with the leu-PET56 allele.

  • KY320 thr- (mating type tester strain) ( Saccharomyces cerevisiae )

  • KY320 a thr- (mating type tester strain) ( Saccharomyces cerevisiae )

  • KY320 Diploid ( Saccharomyces cerevisiae )

  • KY320a ( Saccharomyces cerevisiae )

    Mat a, ura3-52, lys2-801, ade2-101, trp1- 1, his3 200, GAL+, leu2::PET56. Similar to KY117, constructed by replacing the leu2 locus of KY117 with the leu-PET56 allele.

  • KY522 (KY55 ope2-1) ( Saccharomyces cerevisiae )

    KY55 = KY162 (YR 21-Sc2782 TRP1+ his3- 13)KY162 = Mat a, ura3-52, trp1-289, his3- 200, can1. Crunchy mutantHIS+ revertant.

  • KY523 (KY55 ope3-1) ( Saccharomyces cerevisiae )

    KY55 = KY162 (YR 21-Sc2782 TRP1+ his3- 13)KY162 = Mat a, ura3-52, trp1-289, his3- 200, can1. Crunchy mutantHIS+ revertant.

  • KY803 ( Saccharomyces cerevisiae )

    Ura3-52 trp1- 1 leu2-PET56 gal2 gcn4- 1.

  • KY803 a ( Saccharomyces cerevisiae )

    Ura3-52 trp1- 1 leu2-PET56 gal2 gcn4- 1.

  • KY803 not1-2 (MY28 or MY8) ( Saccharomyces cerevisiae )

    Published as MY28, stored as MY8.

  • KY803 not2-1 (MY27 or MY16) ( Saccharomyces cerevisiae )

    Published as MY27, stored as MY16.

  • KY803 not2-4 (MY22 or MY15) ( Saccharomyces cerevisiae )

    Published as MY22, stored as MY15.

  • KY803 not3-2 (MY25 or MY11) ( Saccharomyces cerevisiae )

    Published as MY25, stored as MY15.

  • KY803 not4-1 (MY20 or MY13) ( Saccharomyces cerevisiae )

    Published as MY20, stored as MY13.

  • KY803 TRP1::his3 (Mat ) ( Saccharomyces cerevisiae )

  • KY803 TRP1::his3 (Mat a) ( Saccharomyces cerevisiae )

  • KY898 ( Saccharomyces cerevisiae )

    Mat a, ura3-52, lys2-801, ade2-101, leu2-PET56, trp1 1, his3-303.

  • KY898 ( Saccharomyces cerevisiae )

    Mata ura3052 kys2-801 ade2-101 leu2-PET56 trp1-delta1 his3-303.

  • KY902 ( Saccharomyces cerevisiae )

    Isogenic to KY 898 (Mata ura3052 kys2-801 ade2-101 leu2-PET56 trp1-delta1 his3-303) but acr1-delta1.

  • KY905 ( Saccharomyces cerevisiae )

    Isogenic to KY898 but acr1- 1 gcn4::TRP1.

  • KY905 ( Saccharomyces cerevisiae )

    Isogenic to KY 898 (Mata ura3052 kys2-801 ade2-101 leu2-PET56 trp1-delta1 his3-303) but acr1-delta1 gcn4::TRP1.

  • L40 for 2-hybrid ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 LYS2::lexA-HIS3 URA3::lexA-LacZ, for 2-hybrid.

  • L577 HA-Rpb3 GAL1-YLR454 ( Saccharomyces cerevisiae )

    MAT HA-Rpb3 GAL1-YLR454 spt16-197 his4-912 lys2-128 ura3-52. Parent strain was provided by Fred Winston.

  • L577 HA-Rpb3 YLR454 ( Saccharomyces cerevisiae )

    Parent strain was provided by Fred Winston. MAT HA-Rpb3 spt16-197 his4-912 lys2-128 ura3-52.

  • L9FT4 ( Saccharomyces cerevisiae )

  • L9FT4 ( Saccharomyces cerevisiae )

  • L9FT4 tup1::LEU2 ( Saccharomyces cerevisiae )

    From S. Chatterjee.

  • L9FT4a ( Saccharomyces cerevisiae )

    His3 locus contains a single LexA operator upstream of the TR TATA element.

  • MAP19 = W303-1A with sko1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP32 W303-1a with hog1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 hog1::KAN MX.

  • MAP34 = SFY526 with cyc8::KAN MX ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ.

  • MAP34 with pGBT9 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ cyc8::KAN MXpGBT9 = ADH1p-GAL4DBD fusion vector, TRP1, Amp, Clontech. Grow on CAA -T.

  • MAP34 with pMP235 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ cyc8::KAN MXpMP235 = SKO1 ORF (SmaI/SalI) in pGBT9, full length Gal4DBD-Sko1 fusion. Grow on CAA -T.

  • MAP34 with pMP257 ( Saccharomyces cerevisiae )

    Grow on CAA -T. MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ cyc8::KAN MXpMP257 = SKO1 (1-315) (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion.

  • MAP34 with pMP258 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ cyc8::KAN MXpMP258 = SKO1 (1-315) E (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion with triple mutations of Hog1 phosphorylation sites. Grow on Caa -T.

  • MAP34 with pMP259 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ cyc8::KAN MXSKO1 ORF (SmaI/SalI) in pGBT9, Gal4DBD-Sko1 fusion with triple mutations of Hog1 phosphorylation sites. Grow on Caa -T.

  • MAP36 = W303-1A with hog1::KAN MX, 3HA-SKO1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP37 = W303-1A with 3HA-SKO1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP51 = W303-1A with 3HA-HOG1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP54 = W303-1A with sko1::KAN MX, 3HA-HOG1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP57 = SFY526 with hog1::KAN MX ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ.

  • MAP57 with pGBT9 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ hog1::KAN MXpGBT9 = ADH1p-GAL4DBD fusion vector, TRP1, Amp, Clontech. Grow on Caa -T.

  • MAP57 with pMP235 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ hog1::KAN MXpMP235 = SKO1 ORF (SmaI/SalI) in pGBT9, full length Gal4DBD-Sko1 fusion. Grow on Caa -T.

  • MAP57 with pMP257 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ hog1::KAN MXpMP257 = SKO1 (1-315) (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion. Grow on Caa -T.

  • MAP57 with pMP258 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ hog1::KAN MXpMP258 = SKO1 (1-315) E (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion with triple mutations of Hog1 phosphorylation sites. Grow on Caa -T.

  • MAP57 with pMP259 ( Saccharomyces cerevisiae )

    Grow on Caa -T. MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ hog1::KAN MXSKO1 ORF (SmaI/SalI) in pGBT9, Gal4DBD-Sko1 fusion with triple mutations of Hog1 phosphorylation sites.

  • MAP59 = W303-1A with tup1::KAN MX, 3HA-SKO1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 Very clumpy.

  • MAP62 = W303-1A with hog1::KAN MX, ADA2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP63 = W303-1A with sko1::KAN MX, ADA2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP64 = W303-1A with hog1::KAN MX, SWI2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP65 = W303-1A with sko1::KAN MX, SWI2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 .

  • MAP66 = W303-1A with tup1::KAN MX, ADA2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 Clumpy.

  • MAP67 = W303-1A with tup1::KAN MX, SWI2-18myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 Clumpy.

  • MAP72 W303-1a with nha1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 nha1::KAN MX.

  • MAP73 W303-1a with NHA1-HA-KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 NHA1-HA-KAN MX.

  • MAP75 W303-1a with tok1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 tok1::KAN MX.

  • MAP76 W303-1a with ena1-4::HIS3, nha1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 ena1-4::HIS3, nha1::KAN MX.

  • MAP78 W303-1a with ena1-4::HIS3, nha1::loxP, hog1::KAN MX ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 ena1-4::HIS3, nha1::loxP, hog1::KAN MX.

  • MaV103 (2 hybrid host strain) ( Saccharomyces cerevisiae )

  • MAV103 for 2-hybrid ( Saccharomyces cerevisiae )

    MATalpha ade2-101 his3-D200 leu2-3,122 trp1-901 gal4D, gal80D, SPAL10::URA3, GAL1::lacZ, GAL1::HIS3@LYS2, canIR, cyh2R. For 2-hybrid.

  • MBY101 = FY834 made HIS3(+) #5 ( Saccharomyces cerevisiae )

    Stock number 5. Made HIS3+ by gene repair with pUC2676.

  • MBY102 = FY833a made HIS3(+) #7 ( Saccharomyces cerevisiae )

    Stock number 7. Made HIS3+ by gene repair with pUC2676 and gcn4 by two-step deletion. Transformed with yCP88-C163 GCN4 plasmid.

  • MBY103 = Diploid of FY834/FY833 #1 ( Saccharomyces cerevisiae )

    Stock number 1.

  • MBY104 = FY833a made HIS3(+) #6 ( Saccharomyces cerevisiae )

    Stock number 6. Made HIS3(+) by gene repair with pUC2676.

  • MBY105 = FY834 made HIS3(+) #8 ( Saccharomyces cerevisiae )

    Stock number 8. Made HIS3(+) by gene repair with pUC 2676 and gcn4 with two-step deletion.

  • MBY106 #53 ( Saccharomyces cerevisiae )

    Same as MBY102 but also transformed with MB3 his3 promoter:lacZ reporter plasmid. Stock number 53.

  • MBY107 #54 ( Saccharomyces cerevisiae )

    Same as MBY106 except also transformed with YCplac22-TBP. Stock number 54.

  • MBY108 #55 ( Saccharomyces cerevisiae )

    Same as MBY107 except that his strain is GCN4(+). Stock number 55.

  • MBY109 = PSY316 made HIS3(+) #73 ( Saccharomyces cerevisiae )

    Made HIS3(+) by gene repair with pUC2676 and gcn4 by two-step deletion. Stock number 73.

  • MBY110 = MBY109 but also ada2 #82 ( Saccharomyces cerevisiae )

    Stock number 82.

  • MBY112 = MaV103 with sds3::LEU disruption #85 ( Saccharomyces cerevisiae )

    Stock number 85.

  • MBY113 = MaV103 with ada2::LEU2 disruption #86 ( Saccharomyces cerevisiae )

    Stock number 86.

  • MBY114 = MaV103 with gcn5::LEU disruption #87 ( Saccharomyces cerevisiae )

    Stock number 87.

  • MBY115 (see genotype for description) #89 ( Saccharomyces cerevisiae )

    FY833a or FY834 (mating type unknown) with dr1::LEU disruption. Stock number 89.

  • MBY116 = not2-4 with sds3::LEU disruption #108 ( Saccharomyces cerevisiae )

    Stock number 108.

  • MBY117 = not2-4 with gcn5::LEU disruption #109 ( Saccharomyces cerevisiae )

    Stock number 109.

  • MBY118 = not2-4 with ada2::LEU disruption #111 ( Saccharomyces cerevisiae )

    Stock number 111.

  • MBY119 = KY803 with sds3::LEU disruption #110 ( Saccharomyces cerevisiae )

    Stock number 110.

  • MBY120 = LY803 with gcn5::LEU disruption #113 ( Saccharomyces cerevisiae )

    Stock number 113.

  • MBY121 = KY803 with ada2::LEU disruption #112 ( Saccharomyces cerevisiae )

    Stock number 112.

  • MBY122 = KY803 with spt8::LEU disruption #114 ( Saccharomyces cerevisiae )

    Stock number 114.

  • MBY123 = PSY316 with spt8::LEU disruption #115 ( Saccharomyces cerevisiae )

    Stock number 115.

  • MKY100 ( Saccharomyces cerevisiae )

    L9FT4a - replaced Lex-L9-HIS3 locus with HIS3-A5-SLOP (6 LexOp?). HIS3-A5 is basal promoter with Tc, TR, and cloning sites. See Fig. 1, Iyer and Struhl, MCB 15:7059-7066 (1995).

  • MKY101 ( Saccharomyces cerevisiae )

  • MKY102 ( Saccharomyces cerevisiae )

  • MKY103 ( Saccharomyces cerevisiae )

  • MKY104 = FT4a-tbp::LEU2 ( Saccharomyces cerevisiae )

  • MKY106 = FT5 -tbp::LEU2 ( Saccharomyces cerevisiae )

  • MKY108 = L9FT4a (ade-) ( Saccharomyces cerevisiae )

  • MKY109 = L9FT5 (ade-) ( Saccharomyces cerevisiae )

  • MKY110 = KY320 with repaired ADE locus ( Saccharomyces cerevisiae )

    Repaired ADE locus by transforming with pSH24 (ADE2 marker in pBluescript).

  • MKY111 ( Saccharomyces cerevisiae )

    TAF30 knockout strain in KY114a background. Transformed with YI211-TAF30 digested with BstEII. Also called K-N/2.

  • MKY112 = MKY108-tbp::ADE2 ( Saccharomyces cerevisiae )

  • MKY114 = MKY109-tbp::ADE2 ( Saccharomyces cerevisiae )

  • MKY115 ( Saccharomyces cerevisiae )

  • MM101 (same as ZM117 tbp::LEU2 trp1 ura 3-52) ( Saccharomyces cerevisiae )

    TBP on URA3/CEN.

  • MSY104 = FY406 FLAG-H2B asf1::KanMX ( Saccharomyces cerevisiae )

    FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2. Deletion verified by PCR, exhibits slow growth.

  • MSY152 = JDy51 Asf1-18myc ( Saccharomyces cerevisiae )

    Trp+. Checked by PCR and Western.

  • MSY154 = FY406 FLAG-H2B GAL1-YLR454 (Ura+) ( Saccharomyces cerevisiae )

    FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2.

  • MSY157 = MSY152 with GAL1-YLR454 (Ura+) ( Saccharomyces cerevisiae )

  • MSY159 = FY406 FLAG-H2B asf1::KanMX GAL1-YLR454 (Ura+) ( Saccharomyces cerevisiae )

    FY406 is MATa, hta1-htb1 ::LEU2, hta2-htb2 ::TRP1, leu2- 1, ura3-52, trp1- 63, his3- 200pJH23 (FB1251) HIS3 AMP CEN HTA1 FLAG-HTB2. Grow on -URA, parent strain without asf1 deletion is MSY154.

  • pLS2 BY 2 TBP-PolIII mutant ( Saccharomyces cerevisiae )

  • PSY316 ada3 #69 ( Saccharomyces cerevisiae )

    Stock number 69.

  • PSY316 ada2 #47 ( Saccharomyces cerevisiae )

    Stock number 47.

  • PSY316 gcn5 #66 ( Saccharomyces cerevisiae )

    Stock number 66.

  • RMY200 ( Saccharomyces cerevisiae )

    MATa ade2-101 his3-200 lys2-801 trp1-901 ura3-52 hht1-hhf1::LEU2 hht2-hhf2::HIS3 pRM200 [pRS316 HHT2-HHF2]. .

  • RY3 (SRB10+) ( Saccharomyces cerevisiae )

    From R. Young, through M. Lee.

  • RY5 ( srb10) ( Saccharomyces cerevisiae )

    From R. Young, through M. Lee.

  • SFY26 with pGBT9 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZpGBT9 = ADH1p-GAL4DBD fusion vector, TRP1, Amp, Clontech. Grow on CAA -T.

  • SFY26 with pMP235 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZpMP235 = SKO1 ORF (SmaI/SalI) in pGBT9, full length Gal4DBD-Sko1 fusion. Grow on CAA -T.

  • SFY26 with pMP257 ( Saccharomyces cerevisiae )

    Grow on CAA -T. MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZpMP257 = SKO1 (1-315) (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion.

  • SFY26 with pMP258 ( Saccharomyces cerevisiae )

    Grow on CAA -T. MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZpMP258 = SKO1 (1-315) E (SmaI/SalI) in pGBT9, Gal4DBD-Sko1(1-315) fusion with triple mutations of Hog1 phosphorylation sites.

  • SFY26 with pMP259 ( Saccharomyces cerevisiae )

    Grow on CAA -T. MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZpMP259 = SKO1 ORF (SmaI/SalI) in pGBT9, Gal4DBD-Sko1 fusion with triple mutations of Hog1 phosphorylation sites.

  • SFY526 ( Saccharomyces cerevisiae )

    MAT ura3 his3 ade2 lys2 trp1 leu2 gal4 gal80 URA3::GAL1-lacZ.

  • SH12 - 162 sua7::ADE2 TBP and IIB on YCp33 ( Saccharomyces cerevisiae )

    Based on KY320a (Mat a, ura3-52, lys2-801, ade2-101, trp1- 1, his3 200, GAL+, leu2::PET56).

  • SKY697 W303-1a with ena1-4::HIS3 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 ena1::HIS3.

  • SLY95 (SRB10+) ( Saccharomyces cerevisiae )

    From R. Young, through D. Tzamarias.

  • SLY96 (srb10-3) ( Saccharomyces cerevisiae )

    From R. Young, through D. Tzamarias.

  • SPY - ADE ( Saccharomyces cerevisiae )

    MATa ade2::hisG his3::ADE2 leu2::PET56 met17-0 trp1-63 ura3-0.

  • SPY - ADE 3HA - TAF130 ( Saccharomyces cerevisiae )

    MATa ade2::hisG his3::ADE2 leu2::PET56 met17-0 trp1-63 ura3-0.

  • SPY-ADE for promoter-HIS3 fusions ( Saccharomyces cerevisiae )

    MATa ade2::hisG his3::ADE2 leu2::PET56 met17-D0 trp1-D63 ura3-D0.

  • SPY-ADE 3HA-TBP Gcr1-9myc ( Saccharomyces cerevisiae )

    Trp+.

  • SPY-ADE 3HA-TBP Gcr2-9myc ( Saccharomyces cerevisiae )

    Trp+.

  • THC42 sir3::HIS3 ( Saccharomyces cerevisiae )

    mat::TRP1 HMLalpha hmr::rA1A2 ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sir3::HIS3 lys2 bar1::hisG.

  • THC67 GAL10P-SIR3HA::URA3 ( Saccharomyces cerevisiae )

    Probably contains 2 copies of GAL-SIR3HA expression vector, possibly three copies.. mat::TRP1 HMLalpha hmr ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1::GAL10P-SIR3HA::URA3 lys2 bar1::hisG.

  • THC70 ( Saccharomyces cerevisiae )

    Probably contains 2 copies of GAL-SIR3HA expression vector, possibly three copies.. mat::TRP1 hml::loxP-kanMX-loxP hmr::rHMRa ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1(::GAL-SIR3HA::URA3) sir3::HIS3 lys2 bar1::hisG.

  • THC78 sir3::HIS3 ( Saccharomyces cerevisiae )

    Mat::TRP1 hml::loxP-(kanMX-loxP)2 hmr::rHMRa (or hmr::rA1A2 not sure) ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 sir3::HIS3 lys2 bar1::hisG.

  • UCC1111 dot1 deletion His5+ ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 dot1::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 dot1::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 HHF2-HHT2 K79A mutation on TRP/CEN plasmid ( Saccharomyces cerevisiae )

    UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pF13 (TRP/CEN)-HHT2-HHF2 K79A]. WT obtained from Mark Parthunplasmid F13 obtained from Sharon Dent Roth.

  • UCC1111 HHF2-HHT2 K79P mutation on TRP/CEN plasmid ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pF13 (TRP/CEN)-HHT2-HHF2 K79A].

  • UCC1111 HHF2-HHT2 K79Q mutation on TRP/CEN plasmid ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pF13 (TRP/CEN)-HHT2-HHF2 K79A].

  • UCC1111 rad6::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 rtf1::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 set1::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 sir2::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 sir3::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 sir4::kan ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC1111 WT (TRP+, ADE-, HHF2-HHT2 on TRP/CEN plasmid) ( Saccharomyces cerevisiae )

    UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pF13 (TRP/CEN)-HHT2-HHF2]. WT obtained from Mark Parthunplasmid F13 obtained from Sharon Dent Roth.

  • UCC1111 WT (TRP-, ADE+) ( Saccharomyces cerevisiae )

    WT obtained from Mark Parthun. UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • UCC506 ( Saccharomyces cerevisiae )

  • UCC506 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • UCC506 sin3::HIS3 ( Saccharomyces cerevisiae )

  • UCC508 ( Saccharomyces cerevisiae )

  • UCC508 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • UCC508 sin3::HIS3 ( Saccharomyces cerevisiae )

  • UCC510 ( Saccharomyces cerevisiae )

  • UCC510 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • UCC510 sin3::HIS3 ( Saccharomyces cerevisiae )

  • W1588-4C = W303-1A RAD5 ( Saccharomyces cerevisiae )

    MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 RAD5(does W303-1a have a RAD5 defect?????).

  • W303 AR5 Bre2-9myc [cdc73:kan] ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS). Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 Bre2-9myc [rad6:kan] ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS). Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 Bre2-9myc [rtf1:kan] ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS). Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 Bre2-9myc::TRP ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS). Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 rtf1::kan ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS)Select on G418. Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 set1::TRP ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS)Select on G418. Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 set1::TRP set2,dot1::kan ( Saccharomyces cerevisiae )

    Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5, set 1 deleted by TRP markerset2, dot 1 deleted by Kanamycin marker. W303 AR5 obtained from David Sinclair (Pathology, HMS).

  • W303 AR5 set1::TRP, set2::kan ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS)Select on G418. Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 set1::TRP, set2::kan, dot1::His5 ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS)Select on G418. Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 set2::kan ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS)Select on G418. Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303 AR5 WT ( Saccharomyces cerevisiae )

    W303 AR5 obtained from David Sinclair (Pathology, HMS). Mata, ade2-1, leu2-3, can1-100, trp1-1, ura3-52, his3-11,15, RDN1::ADE2, RAD5.

  • W303-1a GAL1 - YLR454 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 (W303-1a)YIplac204 is TRP+. W303-1a containing 700bp GAL1/10 intergenic segment (in YIplac204) integrated at the 5’ end of YLR454w such that the GAL1-YLR454 joint is at the common ATG.

  • W303-1a HIS3 ( Saccharomyces cerevisiae )

    YPD. MATa ade2 his3 leu2 trp1 ura3.

  • W303-1a HIS3 3xmyc-Gcn4 ( Saccharomyces cerevisiae )

    YPD. MATa ade2 his3 leu2 trp1 ura3.

  • W303-1a HIS3 Gcn4-3xmyc ( Saccharomyces cerevisiae )

    YPD. MATa ade2 his3 leu2 trp1 ura3.

  • W303-1a GAL1-YLR454 (TRP+) ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+). Grow on -TRP medium.

  • W303-1a GAL1-YLR454 (TRP+), hpr1::HIS3 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), hpr1::HIS3.

    Grow on -TRP medium.

  • W303-1a GAL1-YLR454 Hpr1-13myc ( Saccharomyces cerevisiae )

    W303-1a/GAL1-454 containing 13 myc epitopes integrated at the 3’ end of the natural HPR1 locus, and flanked downstream by HIS3Grow on HIS- medium. MATa ade2 his3 leu2 trp1 ura3 (W303-1a)YIplac204 is TRP+.

  • W303-1a GAL1-YLR454 Tho2-13myc ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 (W303-1a)YIplac 204 is TRP+. W303-1a/GAL1-454 containing 13 myc epitopes integrated at the 3’ end of the natural THO2 locus, and flanked downstream by HIS3Grow on HIS- medium.

  • W303-1a GAL1-YLR454, hpr1::HIS3, pJD3 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), hpr1::HIS3pJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, pJD3 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+)pJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, pJD3, HA-Rpb3 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), HA-Rpb3pJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, pJD3, Spt16-3Xmyc ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3XmycpJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, pJD3, Spt16-3Xmyc ( Saccharomyces cerevisiae )

    Grow on -TRP-URA mediumDuplicate of y10-13. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3XmycpJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, Spt16-3xmyc, pJD3 ( Saccharomyces cerevisiae )

    Grow on -TRP, transformants were treated with 2xFOA to loop out URA marker on pJD3. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3xmycp JD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, Spt16-3xmyc, YIplac211 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3xmyc. Grow on -TRP, transformants were treated with 2xFOA to loop out URA marker on YIplac211.

  • W303-1a GAL1-YLR454, Tfg2-3Xmyc, HA-Rpb3, pJD3 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Tfg2-3Xmyc, HA-Rpb3pJD3 is yTFIIS ::hisG-URA3-hisG (PPR2 ).

  • W303-1a GAL1-YLR454, YIplac211 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3Xmyc.

  • W303-1a GAL1-YLR454, YIplac211, hpr1::HIS3 ( Saccharomyces cerevisiae )

    Grow on -TRP-URA medium. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), YIplac211, hpr1::HIS3.

  • W303-1a HA-Rpb3 ( Saccharomyces cerevisiae )

    MATa ade2-1 can1-100 his3-11 leu2-3,112 trp1 ura3-1 HA-Rpb3. YPD.

  • W303-1A msn2::HIS3 msn4::TRP1 ( Saccharomyces cerevisiae )

    MATa, ura3, leu2, his3, trp1 ade2 msn2::HIS3 msn4::TRP1.

  • W303-1a Pob3-3myc ( Saccharomyces cerevisiae )

  • W303-1a Spt16-3myc ( Saccharomyces cerevisiae )

  • W303-1a Tfg2-3myc ( Saccharomyces cerevisiae )

  • W303-1a, GAL1-YLR454 (TRP+), set1::KAN ( Saccharomyces cerevisiae )

    Grow on -TRP. MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), set1::KAN.

  • W303-1a, GAL1-YLR454 (TRP+), Spt16-3xmyc, YIplac211 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 GAL1-YLR454 (TRP+), Spt16-3xmyc, YIplac211 (INT/URA). Grow on -TRP-URA.

  • W303-1a, HA-Hog1 (MAP51) ( Saccharomyces cerevisiae )

    YPD. MATa ade2 his3 leu2 trp1 ura3 HA-Hog1.

  • W303-1a, HA-Hog1 (MAP51), ctk1::KAN ( Saccharomyces cerevisiae )

    YPD. MATa ade2 his3 leu2 trp1 ura3 HA-Hog1 ctk1::KAN.

  • W303-1a, hpr1::HIS3 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 hpr1::HIS3. YPDfrom Katya Strasser.

  • W303-1a, tho2::KAN ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 tho2::KAN. YPD THIS STRAIN IS VERY SICK!! GROW AT RT ON AGAR PLATE - DOESN'T LIKE 30 DEGREES!!from Katya Strasser.

  • WZY42 TEL2 HHT2-HHF2 K79A mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL2 HHT2-HHF2 K79P mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL2 HHT2-HHF2 K79Q mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL2 WT ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL3 HHT2-HHF2 K79A mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL3 HHT2-HHF2 K79P mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL3 HHT2-HHF2 K79Q mutation ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • WZY42 TEL3 WT ( Saccharomyces cerevisiae )

    WT (from S. Roth) contains HHT-HHF2 on TRP/CEN plasmid (F13, from Sharon Roth). Mutations A, P, Q are on TRP/CEN plasmids. The ADE2-TEL(VR) derivative of WZY42 was constructed by integrating EcoRI-linearlized pHR10-6 plasmid DNA and selecting clones on the basis of the colony color phenotype.. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • XF236F8 ( Saccharomyces cerevisiae )

    MAT his3 1 leu2 0 lys2 0 met15 0 trp1 63 ura30 Pdr1:: Pdr1-(HA)3 Gal11:: Gal11::Gal11-(tMyc)3. Constructed by mating strain 207Leu and Gal11-tMyc, then tetrad dissection. 207Leu is a BY4727 derivative that has three copies of HA tag at the C-terminus of Pdr1, with a Leu2 CEN plasmid. Gal11-tMyc was a derivative of Gal11-TAP in BY4741 background with TAP tag replaced by three copies of Myc tag..

  • XY004 = FY833a ade2 ( Saccharomyces cerevisiae )

  • XY005 = FY833a ade2 tbp::Ade2 with BP on URA3 ( Saccharomyces cerevisiae )

  • XY005 gcn5 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out gcn5 with gcn5 ::LEU2.

  • XY005 gcn5 ::LEU2 ( Saccharomyces cerevisiae )

    Derived from yBY105 (???) ura3-52 trp1- 161 lys2- 202 gcn4- 1.

  • XY005 sin4 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out Sin4 with FB1808.

  • XY005 snf2 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out Snf2 with FB2097.

  • XY005 spt3 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out Spt3 with FB652.

  • XY005 spt7 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out spt7 with FB1564.

  • XY005 spt8 ( Saccharomyces cerevisiae )

    XY005 with TBP wt on URA3, knock out Spt8 with FB749.

  • XY101= XY005 with TBP1 on TRP1 ( Saccharomyces cerevisiae )

  • XY102= XY005 with TBP2 on TRP1 ( Saccharomyces cerevisiae )

  • XY103= XY005 with TBP3 on TRP1 ( Saccharomyces cerevisiae )

  • XY104= XY005 with TBP4 on TRP1 ( Saccharomyces cerevisiae )

  • XY106 = XY005 with TBP6 on TRP1 ( Saccharomyces cerevisiae )

  • XY107 = XY005 with TBP7 on TRP1 ( Saccharomyces cerevisiae )

  • XY109 = XY005 with TBP9 on TRP1 ( Saccharomyces cerevisiae )

  • XY110 = XY005 with TBP10 on TRP1 ( Saccharomyces cerevisiae )

  • XY111= XY005 with TBP11 on TRP1 ( Saccharomyces cerevisiae )

  • XY112 = XY005 with TBP wt on TRP1 ( Saccharomyces cerevisiae )

  • XY201 = ySH103 pXY201 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -54 to +30).

  • XY202 = ySH103 pXY202 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -34 to +30).

  • XY203 = ySH103 pXY203 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -7 to +30).

  • XY204 = ySH103 pXY204 ( Saccharomyces cerevisiae )

    SH103 = his3-200 ura3-52 trop1-161 lys2 -202 leu2-1::PET56 gcn4-1 (derived from FY833)his3 promoter deletion on YIp55 (-120 to -105, -83 to +30).

  • XY205 = ySH103 pXY205 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-152 to -105, -83 to +30).

  • XY206 = ySH103 pXY206 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-203 to -105, -83 to +30).

  • XY207 = ySH103 pXY207 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -83 to -54).

  • XY208 = ySH103 pXY208 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -83 to -34).

  • XY209 = ySH103 pXY209 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -83 to -7).

  • XY210 = ySH103 pXY210 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-83 to +30).

  • XY211 = ySH103 pXY211 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -74 to +30).

  • XY213 = ySH103 pXY213 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -83 to +13).

  • XY214 = ySH103 pXY214 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, -105 to -215).

  • XY215 = ySH103 pXY215 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -105, +30 to -83).

  • XY216 = ySH103 pXY216 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter insert into coding region of Ade2 (see map).

  • XY217 = ySH103 pXY217 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -155).

  • XY218 = ySH103 pXY218 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)his3 promoter deletion on YIp55 (-447 to -195).

  • XY219 = ySH103 pXY219 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833)multi hinfI sites replace his3 promoter (-220 to +30).

  • XY220 = ySH103 pXY220 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY221= ySH103 pXY221 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY222= ySH103 pXY222 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY224= ySH103 pXY224 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY230 = ySH103 pXY230 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY232= ySH103 pXY232 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • XY234= ySH103 pXY234 ( Saccharomyces cerevisiae )

    ySH103 = his3- 200 ura3-52 trp1- 161 lys2 - 202 leu2- 1::PET56 gcn4- 1 (derived from FY833).

  • Y1794 ( Saccharomyces cerevisiae )

  • Y1990 ( Saccharomyces cerevisiae )

  • Y1991 ( Saccharomyces cerevisiae )

  • Y2084 ( Saccharomyces cerevisiae )

  • Y2085 ( Saccharomyces cerevisiae )

  • yAG10 - Isogenic to KY898 but ∆acr1∆aca1∆aca2∆hac1 ( Saccharomyces cerevisiae )

  • yAG12 - Isogenic to KY898 but ∆gcn4 ( Saccharomyces cerevisiae )

  • yAG13 - Isogenic to KY898 but A12∆gcn4 ( Saccharomyces cerevisiae )

  • yAG14=KY114 ( Saccharomyces cerevisiae )

    Mat alpha ura3-52 trp1-289 aas-5039 can1-k2.

  • yAG15 - Isogenic to KY114 but ∆gcn4 ( Saccharomyces cerevisiae )

  • yAG16 - Isogenic to KY898 but ∆hal6 ( Saccharomyces cerevisiae )

  • yAG17 - Isogenic to KY898 but hal7::LEU2 ( Saccharomyces cerevisiae )

  • yAG18 - Isogenic to KY898 but ∆hal6 hal7::LEU2 ( Saccharomyces cerevisiae )

  • yAG19 - Isogenic to KY898 but ∆acr1∆aca1∆aca2∆hal6 ( Saccharomyces cerevisiae )

  • yAG1=KY898 ( Saccharomyces cerevisiae )

    MATa ura3-52 lys2-801 ade2-101 leu2-PET56 trp1- 1 his3-303.

  • yAG2 Isogenic to KY898 but ∆aca1 ( Saccharomyces cerevisiae )

  • yAG20 - Isogenic to KY898 but ∆acr1∆aca1∆aca2hal7::LEU2 ( Saccharomyces cerevisiae )

  • yAG21 - Isogenic to KY898 but ∆acr1∆aca1∆aca2∆hal6 hal7::LEU2 ( Saccharomyces cerevisiae )

  • yAG22 - Isogenic to KY898 but hog1::TRP ( Saccharomyces cerevisiae )

  • yAG23 - Isogenic to KY898 but ∆acr1∆aca1∆aca2hog1::TRP ( Saccharomyces cerevisiae )

  • yAG24 - Isogenic to KY898 but tup1::LEU2 ( Saccharomyces cerevisiae )

  • yAG25 - Isogenic to KY898 but ∆acr1 tup1::LEU2 ( Saccharomyces cerevisiae )

  • yAG26 - Isogenic to KY898 but cyc8::LEU2 ( Saccharomyces cerevisiae )

  • yAG27 - Isogenic to KY898 but ∆met28 ( Saccharomyces cerevisiae )

  • yAG28 - Isogenic to KY898 but ∆acr1∆aca1∆aca2∆met28 ( Saccharomyces cerevisiae )

  • yAG29 - Isogenic to KY898 but ∆hot1 ( Saccharomyces cerevisiae )

  • yAG3 - Isogenic to KY898 but ∆aca2 ( Saccharomyces cerevisiae )

  • yAG30 - Isogenic to KY898 but ∆acr1∆aca1∆aca2∆hot1 ( Saccharomyces cerevisiae )

  • yAG4 - Isogenic to KY898 but ∆aca1∆aca2 ( Saccharomyces cerevisiae )

  • yAG5 - Isogenic to KY898 but ∆acr1 ( Saccharomyces cerevisiae )

  • yAG6 - Isogenic to KY898 but ∆acr1∆aca1 ( Saccharomyces cerevisiae )

  • yAG7 - Isogenic to KY898 but ∆acr1∆aca2 ( Saccharomyces cerevisiae )

  • yAG8 - Isogenic to KY898 but ∆acr1∆aca1∆aca2 ( Saccharomyces cerevisiae )

  • yAG9 - Isogenic to KY898 but ∆hac1 ( Saccharomyces cerevisiae )

  • yDH030 = FY1346 +pDH043 ( Saccharomyces cerevisiae )

    FY1346 mat-alpha, ade2, his3, met15, trp1, ura3, pDH043 (Gal4 UAS ~800bp from HIS3). pDH043 Gal1/10 800 bp from His3.

  • yDH031 = FY1346 + pDH044 ( Saccharomyces cerevisiae )

    FY1346 mat-alpha, ade2, his3, met15, trp1, ura3, pDH044 (Gal4 UAS ~100bp from HIS3). pDH044 Gal1/10 130bp from His3.

  • yDH048 = FY1346 3myc-TBP (n-term) ( Saccharomyces cerevisiae )

    All tagging was done by integration of PCR product from plasmid pMPY-3xMYC followed by looping out of URA marker leaving only the 3myc tag at the c-terminus (unless noted as n-term in which case pDH037 was used for PCR). mat-alpha, ade2, his3, met15, trp1, ura3, 3myc-TBP n-term.

  • yDH049 = FY1346 3myc-TAF17 ( Saccharomyces cerevisiae )

    All tagging was done by integration of PCR product from plasmid pMPY-3xMYC followed by looping out of URA marker leaving only the 3myc tag at the c-terminus (unless noted as n-term in which case pDH037 was used for PCR). mat-alpha, ade2, his3, met15, trp1, ura3, 3myc-TAF17.

  • yDH050 = FY1346 3myc-TAF61 ( Saccharomyces cerevisiae )

    All tagging was done by integration of PCR product from plasmid pMPY-3xMYC followed by looping out of URA marker leaving only the 3myc tag at the c-terminus (unless noted as n-term in which case pDH037 was used for PCR). mat-alpha, ade2, his3, met15, trp1, ura3, 3myc-TAF61.

  • yDH102 = 3myc TBP(N) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH103 = 3myc TAF9 (TAF17) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH104 = 3myc TAF13 (TAF19) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH105 = 3myc TAF10 (TAF25) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH106 = 3myc TAF14 (TAF30) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH107 = 3myc TAF11 (TAF40) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH108 = 3myc TAF3 (TAF47) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH109 = 3myc TAF4 (TAF48) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH110 = 3myc TAF6 (TAF60) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH111 = 3myc TAF12 (TAF61) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH113 = 3myc TAF7 (TAF67) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH114 = 3myc TAF5 (TAF90) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH115 = 3myc TAF1 (TAF145) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH116 = 3myc TAF2 (TAF150) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH162 = 3myc Ada1 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH163 = 3myc Ada2 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH164 = 3myc Ada3 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH165 = 3myc GCN5 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH166 = 3myc Spt3 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH168 = 3myc Spt8 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH171 = 3myc Ahc1 ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH172 = 3myc TOA1 (large TFIIA subunit) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH173 = 3myc TOA2 (small TFIIA subunit) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH174 = 3myc Sua7 (TFIIB) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH175 = 3myc TAF8(N) (TAF65) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH254 = 3myc Spt20(N) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH255 = 3myc Tra1(N) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH262 = 3myc Spt7 (N) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH263 = 3myc TAF12 (375-539) (N) (TAF61) ( Saccharomyces cerevisiae )

    Background is ResGen BY4742Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, Gal4.

  • yDH270 = BY4741 delGAL4 3HA-Ace1 ( Saccharomyces cerevisiae )

    Mat a, his3 1, leu2 0, met15 0, ura3 0, Gal4. 3HA-Ace1.

  • yDH271 = BY4741 delGAL4 3HA-Hsf1 ( Saccharomyces cerevisiae )

    Mat a, his3 1, leu2 0, met15 0, ura3 0, Gal4. 3HA-Hsf1.

  • yDH273 = BY4741 3HA-Gal4 ( Saccharomyces cerevisiae )

    Mat a, his3 1, leu2 0, met15 0, ura3 0. 3HA-Gal4.

  • yDH305 = 3myc-Tra1 (n-term) ( Saccharomyces cerevisiae )

    Jerry Workman strain, Tra1 on plasmid. 3myc-Tra1 (n-term).

  • yDH306 = 3myc-Tra1 (n-term) ( Saccharomyces cerevisiae )

    3myc-Tra1 (n-term). Jerry Workman strain, TS2-Tra1 on plasmid.

  • yDH321 = 3myc TBP(N) BY4741 ada2 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH102 (3myc-TBP). MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN.

  • yDH322 = 3myc TFIIB BY4741 ada2 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH174 (3myc-TFIIB). MATalpha, his3 1, leu2 0, ura3 0, gal4::KAN, ada2::KAN.

  • yDH323a = 3myc Taf130 BY4741 ada2 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH130 (3myc-Taf130). MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN.

  • yDH323alpha = 3myc Taf130 BY4741 ada2 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH130 (3myc-Taf130). MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN.

  • yDH324a = 3myc Ada1 BY4741 ada2 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1).

  • yDH324alpha = 3myc Ada1 BY4741 ada2 background ( Saccharomyces cerevisiae )

    MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1).

  • yDH325 = 3myc Tra1 BY4741 ada2 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH255 (3myc-Tra1). MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, ada2::KAN.

  • yDH326a = 3myc TBP (N) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH102 (3myc-TBP). MATa, his3 1, leu2 0, lys2 0, ura3 0, gal4::KAN, spt3::KAN.

  • yDH326alpha = 3myc TBP (N) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH102 (3myc-TBP). MATalpha, his3 1, leu2 0, ura3 0, gal4::KAN, spt3::KAN.

  • yDH327 = 3myc TFIIB BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH174 (3myc-TFIIB). MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN.

  • yDH328a = 3myc TAF130 (TAF145) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH130 (3myc-Taf130). MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN.

  • yDH328a lpha = 3myc TAF130 (TAF145) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH130 (3myc-Taf130). MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN.

  • yDH329a = 3myc Ada1 BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1). MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN.

  • yDH329alpha = 3myc Ada1 BY4741 spt3 background ( Saccharomyces cerevisiae )

    MATalpha, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1).

  • yDH330a= 3myc Tra1 (N) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH255 (3myc-Tra1). MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt3::KAN.

  • yDH330alpha = 3myc Tra1 (N) BY4741 spt3 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH255 (3myc-Tra1). MATalpha, his3 1, leu2 0, lys2 0, ura3 0, gal4::KAN, spt3::KAN.

  • yDH331= 3myc TBP (N) BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH102 (3myc-TBP).

  • yDH332a = 3myc TFIIB BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH174 (3myc-TFIIB).

  • yDH332alpha = 3myc TFIIB BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH174 (3myc-TFIIB).

  • yDH333 = 3myc TAF130 BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH130 (3myc-Taf130).

  • yDH334a = 3myc Ada1 BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1).

  • yDH334alpha = 3myc Ada1 BY4741 spt20 background ( Saccharomyces cerevisiae )

    MATalpha, his3 1, leu2 0, ura3 0, gal4::KAN, spt20::KAN. All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH162 (3myc-Ada1).

  • yDH335 = 3myc Tra1 (N) BY4741 spt20 background ( Saccharomyces cerevisiae )

    All sporulations were screened for del-SAGA, del-Gal4 and myc tag. Mating types as noted on tubes.Mated with yDH255 (3myc-Tra1). MATalpha, his3 1, leu2 0, lys2 0, ura3 0, gal4::KAN, spt20::KAN.

  • yDH407 = BY4742 3myc-Nut1 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Nut1. 3myc-Nut1.

  • yDH408 = BY4742 3myc-Ssn3 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Ssn3. 3myc-Ssn3.

  • yDH409 = BY4742 3myc-Med4 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Med4. 3myc-Med4.

  • yDH410 = BY4742 3myc-Paf1 ( Saccharomyces cerevisiae )

    3myc-Paf1. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Paf1.

  • yDH411 = BY4742 3myc-Cdc73 ( Saccharomyces cerevisiae )

    3myc-Cdc73. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Cdc73.

  • yDH412 = BY4742 3myc-Tfa2 ( Saccharomyces cerevisiae )

    3myc-Tfa2. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Tfa2.

  • yDH413 = BY4742 3myc-Kin28 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Kin28. 3myc-Kin28.

  • yDH414 = BY4742 3myc-Tfb1 ( Saccharomyces cerevisiae )

    3myc-Tfb1. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Tfb1.

  • yDH415 = BY4742 3myc-Rfa1 ( Saccharomyces cerevisiae )

    3myc-Rfa1. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Rfa1.

  • yDH416 = BY4742 3myc-Rfa2 ( Saccharomyces cerevisiae )

    3myc-Rfa2. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Rfa2.

  • yDH417 = BY4742 3myc-Rfa3 ( Saccharomyces cerevisiae )

    3myc-Rfa3. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Rfa3.

  • yDH418 = BY4742 3myc-Spt2 ( Saccharomyces cerevisiae )

    3myc-Spt2. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Spt2.

  • yDH419 = BY4742 3myc-Hmo1 ( Saccharomyces cerevisiae )

    3myc-Hmo1. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1.

  • yDH420 = BY4742 3myc-Hgh1 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hgh1. 3myc-Hgh1.

  • yDH421 = BY4742 3myc-Nhp10 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Nhp10. 3myc-Nhp10.

  • yDH422 = BY4742 3myc-Rpt6 ( Saccharomyces cerevisiae )

    3myc-Rpt6. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Rpt6.

  • yDH423 = BY4742 3myc-Nhp6B ( Saccharomyces cerevisiae )

    3myc-Nhp6B. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Nhp6B.

  • yDH424 = BY4742 del Nhp6A 3myc-Nhp6B ( Saccharomyces cerevisiae )

    3myc-Nhp6B. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, del Rhp6A, 3myc-Nhp6B.

  • yDH441 = BY4242 3myc-Nhp6A ( Saccharomyces cerevisiae )

    3myc-Nhp6A. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Nhp6A.

  • yDH442 + BY4742 3myc-Srb4 ( Saccharomyces cerevisiae )

    3myc-Srb4. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Srb4.

  • yDH442A = BY4742 3myc-Gal11 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Gal11. 3myc-Gal11.

  • yDH443 = BY4742 3myc-Med10 ( Saccharomyces cerevisiae )

    3myc-Med10. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Med10.

  • yDH444 = BY4742 3myc-Sin4 ( Saccharomyces cerevisiae )

    3myc-Sin4. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Sin4.

  • yDH445 = BY4742 3myc-Rsc3 ( Saccharomyces cerevisiae )

    3myc-Rsc3. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Rsc3.

  • yDH446 = BY4742 3myc-Spt20 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Spt20. 3myc-Spt20.

  • yDH447 = BY4742 3myc-Esa1 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Esa1. 3myc-Esa1.

  • yDH448 = BY4742 3mcy-Swi2 ( Saccharomyces cerevisiae )

    3myc-Swi2. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Swi2.

  • yDH449 = BY4742 3myc-Ino80 ( Saccharomyces cerevisiae )

    3myc-Ino80. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Ino80.

  • yDH450 = yDH407 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid)THERE ARE TWO STRAINS NAMED yDH550. BY4741, 3myc-Nut1, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH451 = yDH408 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid)THERE ARE TWO STRAINS NAMED yDH551. BY4741, 3myc-ssn3, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH452 = yDH409 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Med4, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH453= yDH410 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Paf1, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH454 = yDH411 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Cdc73, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH455 = yDH412 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Tfa2, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH456 = yDH413 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-kin28, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH457 = yDH414 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Tfb1, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH458 = yDH415 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Rfa1, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH459 = yDH416 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Rfa2, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH460 = yDH417 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Rfa3, del-Gal4(pFA-KanMX6 plasmid).

  • yDH461 = yDH418 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Spt2, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH462 = yDH419 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Hmo1, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH463 = yDH420 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Hgh2, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH464 = yDH421 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Nhp10, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH465 = yDH422 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Rpt6, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH466 = yDH423 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Nhp6B, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH467 = yDH441 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Nhp6A, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH468 - yDH442 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Srb4, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH469 = yDH442A del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Gal11, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH470 = yDH443 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Med10, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH471 = yDH444 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Sin4, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH472 = yDH445 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Rsc3, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH473 = yDH446 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Spt20, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH474 = yDH447 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    BY4742, 3myc-Esa1, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH475 = yDH448 del-Gal4 (pFA-KanMX6 plasmid) MISSING ( Saccharomyces cerevisiae )

    BY4742, 3myc-Swi2, del-Gal4 (pFA-KanMX6 plasmid). del-Gal4 (pFA-KanMX6 plasmid).

  • yDH476 = yDH449 del-Gal4 (pFA-KanMX6 plasmid) ( Saccharomyces cerevisiae )

    del-Gal4 (pFA-KanMX6 plasmid). BY4742, 3myc-Ino80, del-Gal4 (pFA-KanMX6 plasmid).

  • yDH531 = BY4742 3myc-Taf130 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Taf130. 3myc-Taf130.

  • yDH532 = BY4742 3myc-TBP (n-term) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-TBP. 3myc-TBP (n-term).

  • yDH533 = BY4742 3myc-Ada1 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Ada1. 3myc-Ada1.

  • yDH534 = BY4742 3myc-Spt3 ( Saccharomyces cerevisiae )

    3myc-Spt3. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Spt3.

  • yDH535 = BY4742 3myc-Spt8 ( Saccharomyces cerevisiae )

    3myc-Spt8. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Spt8.

  • yDH536 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    RPS8A (-761/-550) in RPS11B. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, RPS8A (-761/-550) insert into RPS11B.

  • yDH537 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, RPL9A (-588/-220) insert into RPS11B. RPL9A (-588/-220) in RPS11B.

  • yDH538 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    PGK1 (-653/-246) in RPS11B. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, PGK1 (-653/-246) insert into RPS11B.

  • yDH539 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    PYK1 (-844/-586) in RPS11B. Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, PYK1 (-844/-586) insert into RPS11B.

  • yDH540 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, RPS8A (-692/-630) insert into RPS11B. RPS8A (-692/-630) in RPS11B.

  • yDH541 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, RPS8A (-692/-650) insert into RPS11B. RPS8A (-692/-650) in RPS11B.

  • yDH542 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, RPS8A (-692/-650) w/ mutant Rap1 insert into RPS11B. RPS8A (-692/-650) w/ mutant Rap1 in RPS11B.

  • yDH543 = yDH419 (3myc-HMO1) w/insert (see below) ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, vector only insert into RPS11B. vector only.

  • yDH544 = BY4741 del-Hmo1 ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, del-Hmo1 (pFA-KanMX6 plasmid). del-Hmo1 (pFA-KanMX6 plasmid)THIS IS NOT THE CORRECT STRAIN - DOES NOT GROW ON KAN, DOES NOT HAVE SMALL PHENOTYPE. SEE yG4-26 for HMO1 deletion.

  • yDH545 = BY4741 del-Nhp6B ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, del-Nhp6B (pFA-HisMX6 plasmid). del-Nhp6B (pFA-HisMX6 plasmid).

  • yDH546 = BY4741 del-Nhp6A del-Nhp6B ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, del-Nhp6A, del-Nhp6B (pFA-HisMX6 plasmid). del-Nhp6B (pFA-HisMX6 plasmid).

  • yDH547 = BY4742 del-Fpr1 3myc-Hmo1 ( Saccharomyces cerevisiae )

    Mat alpha, his3 1, leu2 0, lys2 0, ura3 0, 3myc-Hmo1, del-Fpr1. 3myc-Hmo1.

  • yDH548 = BY4743 del-Fhl1 3Myc-Hmo1 ( Saccharomyces cerevisiae )

    Joe Wade's BY4743, del-Fhl1sporulated to (a, del-met, del-lys). 3myc-Hmo1.

  • yDH549 = BY4741 del-Nhp6B c-term (159-300) ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, del-Nhp6B (159-300). del-Nhp6B c-term(159-300).

  • yDH550 = BY4741 del-Nhp6A, del-NHP6B c-term (159-300) ( Saccharomyces cerevisiae )

    MATa, his3 1, leu2 0, ura3 0, met15 0, del-Nhp6A, del-NHP6B c-term (159-300). del-NHP6B c-term (159-300).

  • yDH551 = yDH441 3myc-Nhp6A del-Nhp6B c-term(159-300) ( Saccharomyces cerevisiae )

    BY4741, 3myc-Nhp6A, del-Nhp6B c-term(159-300). del-Nhp6B c-term(159-300).

  • yDH552 = WT 723B ???? ( Saccharomyces cerevisiae )

    No information. Not sure of strain name, can't read writing on tube.

  • yDH553 = MH 723B ???? ( Saccharomyces cerevisiae )

    No information. Not sure of strain name, can't read writing on tube.

  • yDH555 = MM 723B ???? ( Saccharomyces cerevisiae )

    No information. Not sure of strain name, can't read writing on tube.

  • yHN1 = FT4 RSC1 - 9 myc ( Saccharomyces cerevisiae )

    Protein tagged at C-terminus with 9myc::TRP1 cassette (Cosma et al.. 1999. Cell 97:299-311). Mat a ura3-52 trp1 63 his3 200 leu2::PET56 Rsc1-9myc::TRP.

  • yHN10 = FT4 RSC8 mbp1 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc mbp1::His5+. http://web.wi.mit.edu/young/RSC.

  • yHN13 = FT4 RSC8 hir1 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc hir2::His5+. http://web.wi.mit.edu/young/RSC.

  • yHN14 = FT4 RSC8 hir2 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc hir2::His5+. http://web.wi.mit.edu/young/RSC.

  • yHN15 = FT4 RSC8 ure2 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc ure2::His5+small colonies. http://web.wi.mit.edu/young/RSC.

  • yHN2 = FT4 RSC2 - 9 myc ( Saccharomyces cerevisiae )

    Protein tagged at C-terminus with 9myc::TRP1 cassette (Cosma et al.. 1999. Cell 97:299-311). Mat a ura3-52 trp1 63 his3 200 leu2::PET56 Rsc2-9myc::TRP.

  • yHN3 = FT4 RSC3 - 9 myc ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 Rsc3-9myc::TRP. Protein tagged at C-terminus with 9myc::TRP1 cassette (Cosma et al.. 1999. Cell 97:299-311).

  • yHN4 = FT4 RSC8 - 9 myc ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 rsc8-9myc::TRP. Protein tagged at C-terminus with 9myc::TRP1 cassette (Cosma et al.. 1999. Cell 97:299-311).

  • yHN7 = FT4 RSC8 Swi4 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc swi4::His5+. http://web.wi.mit.edu/young/RSC.

  • yHN8 = FT4 RSC8 Swi5 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc swi5::His5+. http://web.wi.mit.edu/young/RSC.

  • yHN9 = FT4 RSC8 Swi6 KO ( Saccharomyces cerevisiae )

    Mat a ura3-52 trp1 63 his3 200 leu2::PET56 RSC8-9myc swi6::His5+sick. http://web.wi.mit.edu/young/RSC.

  • YHT+B16(1) = YKT2 + p414 CYC1 -Tet LVP 3HA ( Saccharomyces cerevisiae )

    CAA-T. Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5).

  • YHT+B17(1) = YKT2 + p414 CYC1 ( Saccharomyces cerevisiae )

    CAA-T. Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5).

  • YHT+B5(1) = YKT2 + p414 ADH Tet 3HA UN596 ( Saccharomyces cerevisiae )

    CAA-T. Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5).

  • YHTR13(1) =YKT1R + p414ADH ( Saccharomyces cerevisiae )

    CAA-T. Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5).

  • YHTR34(1) = YKT1R + p414 ADH Tet 3HA UN596 ( Saccharomyces cerevisiae )

    CAA-T. Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5).

  • YJL102-K36R (parent strain is RMY200) ( Saccharomyces cerevisiae )

    MATa ade2-101 his3-200 lys2-801 trp1-901 ura3-52 hht1-hhf1::LEU2 hht2-hhf2::HIS3 pJWL116 [pRS316 hht2 K26R-HHF2]. This strain isn't actually mentioned in the reference. From what I can figure out, pJWL116 = pRM200 with K36R mutation in hht2..

  • YKT1R (Rap1 site at HIS3) ( Saccharomyces cerevisiae )

    Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5)YIp211HisTet Rap (URA3).

  • YKT2 (lacks activator sites upstream of HIS3 core) ( Saccharomyces cerevisiae )

    Mat alpha, ura3-52, trp1- 63, his3- 200, leu2::PET56 (FT5)YIp211HisTet (URA3).

  • YKY5A = WZY42 Myc-H4 ( Saccharomyces cerevisiae )

    Made by YKK, similar to H. Ng strain which he didn't leave in labNOTE: THE TUBE IS MARKED WITH THE INCORRECT PARENT STRAIN (UCC1111). WZY42 IS CORRECT!. WZY42[MATa, ura3-52, lys2-801, ade2-101, trp1-63, his3-200, leu2-1, hht1-hhf1::pWZ405-F2F9-LEU2, hht2-hhf2::pWZ403-F4F10-HIS3,Ycp50-cpyII (HHT2-HHF2)].

  • YKY6B = UCC1111 myc-H4 ( Saccharomyces cerevisiae )

    Made by YKK, should be identical to H. Ng strain . UCC1111 [MATalpha, ade2::his3-200, leu2-0, lys2-0, met15-0, trp1-63, ura3-0, adh4::URA3-TEL (VII-L), hhf2-hht2::MET15, hhf1-hht1::LEU2, pRS412 (ADE2 CEN ARS)-HHF2-HHT2].

  • yLAS1 (BY 2 mat a wt TBP/URA3) ( Saccharomyces cerevisiae )

    Mat a, ura3-52, lys2-801, ade2-101, trp1- 1, GAL+, leu2::PET56 spt15 deleted between Xba I and HindIII. Carries YCp86 (CEN/URA3) with yeast TFIID gene cloned as a 2.4 kb EcoRI-BamHI fragment). Derived from KY320.

  • yLAS3 = BY 2 MATa wtTBP/TRP1 (YCp22) ( Saccharomyces cerevisiae )

    Mat a, ura3-52, lys2-801, ade2-101, trp1- 1, GAL+, leu2::PET56 spt15 deleted between Xba I and HindIII.

  • yLF20 (FT4 yap1) ( Saccharomyces cerevisiae )

  • yLF22 (FT4 yap2) ( Saccharomyces cerevisiae )

  • yLF25 (FT4 yap5) ( Saccharomyces cerevisiae )

  • yLF26 (FT4 yap1 yap5) ( Saccharomyces cerevisiae )

  • yLF29 (FT4 yap1 yap5) ( Saccharomyces cerevisiae )

  • yLF30 (FT4 yap2 yap5) ( Saccharomyces cerevisiae )

  • yLF50 (FT4 yap1 yap2 yap5) ( Saccharomyces cerevisiae )

  • yLF51 (FT4 yap4::TRP1) ( Saccharomyces cerevisiae )

  • yLF52 (FT4 yap1 yap4::TRP1) ( Saccharomyces cerevisiae )

  • yLF53 (FT4 yap2 yap4::TRP1) ( Saccharomyces cerevisiae )

  • yLF54 (FT4 yap4::TRP1 yap5) ( Saccharomyces cerevisiae )

  • yLF55 (FT4 yap1 yap2 yap4::TRP1 ( Saccharomyces cerevisiae )

  • yLF56 (FT4 yap1 yap4::TRP1 yap5) ( Saccharomyces cerevisiae )

  • yLF57 (FT4 yap2 yap4::TRP1 yap5) ( Saccharomyces cerevisiae )

  • yLF58 (FT4 yap1 yap2 yap4::TRP1 yap5) ( Saccharomyces cerevisiae )

  • yLK15 = yLK20 = W303-1a +HA3-TAF17 ( Saccharomyces cerevisiae )

    MATa ade2-1 can1-100 his3-11 leu2-3,112 trp1 ura3-1. Constructed using INT/URA pL25 [YIp211 with PstI-EcoRI fragment containing full TAF17 sequence (-692 to +961) with HA epitopes inserted at codon 3].

  • yLK16 = yLK21 = W303-1a +HA3-TAF150 ( Saccharomyces cerevisiae )

    MATa ade2-1 can1-100 his3-11 leu2-3,112 trp1 ura3-1. Constructed using INT/URA pL27 [YIp211 with PstI-EcoRI fragment containing TAF150 sequence (+2860 to +5179) with HA epitopes inserted just before the stop codon].

  • yLK20 = W303-1a +HA3-TAF17 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPD.

  • yLK21 = W303-1a + HA3-TAF150 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPD.

  • yLK25 = W303-1a + 3xHA TBP Mot1-Myc9-KLTRP1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPDKLTRP1 is K. lactis TRP1.

  • yLK27 = W303-1a + Mot1-Myc9-KLTRP1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPDKLTRP1 is K. lactis TRP1.

  • yLK28 = W303-1a + TFIIB-Myc9-KLTRP1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPD.

  • yLK29 = W303-1a + 3xHA TBP TFIIB-Myc9-KLTRP1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPDKLTRP1 is K. lactis TRP1.

  • yLK3 = W303-1A with untagged TBP ( Saccharomyces cerevisiae )

    TBP/L deltaC on YIplac211 (URA3). See attached information sheet. Grow on CAA -URA.

  • yLK30 = W303-1a + TFIIB - 3xHA ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPD.

  • yLK31 = W303-1a + TFIIB - 3xHA Mot1-Myc9-KLTRP1 ( Saccharomyces cerevisiae )

    MATa ade2 his3 leu2 trp1 ura3 . YPDKLTRP1 is K. lactis TRP1.

  • yLK4 = W303-1a with 3-HA tagged TBP ( Saccharomyces cerevisiae )

    3-HA TBP on YIplac211 (URA3). See attached information sheet.W303-1a is MATa ade2 his3 leu2 trp1 ura3 . Grow on CAA -URA.

  • yML2 TBPm3 mutant allele Sc3765 ( Saccharomyces cerevisiae )

    Published as yML2, stored as yML11 in M. Lee’s original stocks. Derived from KY320 (Mata or alpha, ura3-52, lys2-801, ade2-101, trp1- 1, his3 200, GAL+, leu2::PET56). HIS3 locus replaced with TGTAAA-containing allele Sc3765 (no Gcn4 binding site)..

  • yML3 TBPm3 mutant allele Sc3745 ( Saccharomyces cerevisiae )

    Derived from KY320 (Mata or alpha, ura3-52, lys2-801, ade2-101, trp1- 1, his3 200, GAL+, leu2::PET56). HIS3 locus replaced with TGTAAA-containing allele Sc3745 (with Gcn4 binding site).. Published as yML3, stored as yML12 in M. Lee’s original stocks.

  • yML54 (TBP mutant V161A) ( Saccharomyces cerevisiae )

  • yML55 (TBP mutant F148L) ( Saccharomyces cerevisiae )

  • yML6 ( Saccharomyces cerevisiae )

    Published as yML6, stored as yML40 in Lee strains. A5 8710:: 2 TRP (TBP-WT).

  • yML7 ( Saccharomyces cerevisiae )

    3660:: 2 TRP (TBP-WT). Published as yML7, stored as yML32 in Lee strains.

  • YPH250 ( Saccharomyces cerevisiae )

  • YPH250 rpd3::HIS3 ( Saccharomyces cerevisiae )

  • YPH250 sin3::HIS3 ( Saccharomyces cerevisiae )

  • YPH499 ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1.

  • YPH499 CycC-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med14-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med15-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med17-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med19-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med3-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YPH499 Med7-TAP ( Saccharomyces cerevisiae )

    MATa ura3-52 his3- 200 ade2-101uaa trp1- 63 lys2-801uag leu2- 1. C-term TAP tag introduces TRP+ marker.

  • YSB524 HA-KIN28 3myc-SET1 ( Saccharomyces cerevisiae )

    Grow at RT (25 degrees C) in 100 ml flask (CAA-U-T). They don't grow well in tubes.. ura3-1 leu2-3, 112 trp1-1 kin28 ::LEU2 ade2-1 ade3-22 can1-100 with pRS314(TRP1) HA-KIN28 ADH1 3myc-SET1 (URA).

  • YSB524 HA-kin28-16 3myc-SET1 ( Saccharomyces cerevisiae )

    ura3-1 leu2-3, 112 trp1-1 kin28 ::LEU2 ade2-1 ade3-22 can1-100 with pRS314(TRP1) HA-kin28-16 (N123D, P206L, V232A, L293S) ADH1 3myc-SET1 (URA). Grow at RT (25 degrees C) in 100 ml flask (CAA-U-T). They don't grow well in tubes. Temperature sensitive at 37 degrees..

  • YSB592 HA-kin28 (T17D) 3myc-SET1 ( Saccharomyces cerevisiae )

    ura3-1 leu2-3, 112 trp1-1 his3-11 kin28 ::LEU2 ade2-1 ade3-22 can1-100 with pRS314(TRP1) HA-kin28-(T-17D) ADH1 3myc-SET1 (URA) GROWS VERY SLOWLY!!!. Grow at RT (25 degrees C) in 100 ml flask (CAA-U-T). They don't grow well in tubes. .

  • ySH1 (KY320, TFIIB sua7::ADE2)/plasmid SH30 ( Saccharomyces cerevisiae )

    URA3-52, trp1 1, his3 200, leu2-1, lys2-1, TFIIB sua7::ADE2. Strain constructed by S. Chou.

  • ySH1/plasmid pPK1 ( Saccharomyces cerevisiae )

    C-terminally, 3-HA tagged TFIIB supplied on plasmid pPK1 under natural TFIIB promoter. URA3-52, trp1 1, his3 200, leu2-1, lys2-1,TFIIB sua7::ADE2Plasmid is CEN/TRP.

  • ySH1/pRS316IIB ( Saccharomyces cerevisiae )

    IIB-262. KY320 a sua7::ADE2 (pRS316yIIB).

  • ySH101 (833P) = FY833 leu2-1:PET56 ( Saccharomyces cerevisiae )

  • ySH102 (833 g) = FY833 gcn4 ( Saccharomyces cerevisiae )

  • ySH103 (833 gP) = FY833 leu1-1:PET56 gcn4 ( Saccharomyces cerevisiae )

  • ySH104 (833 gPH) ( Saccharomyces cerevisiae )

    This strain should be HIS+. S288C background - MAT ura3-52 lys2 202 trp1 161 ade8 leu2 1 leu1-1:PET56 gcn4.

  • ySH105 = ySH103 A5-7321-his3 ( Saccharomyces cerevisiae )

  • ySH106 = ySH103 TR-TC-his3 ( Saccharomyces cerevisiae )

  • ySH107 = ySH103 TR-TR-his3 ( Saccharomyces cerevisiae )

  • ySH108 = ySH103 8999(TC)-8440(TATAA)-his3 ( Saccharomyces cerevisiae )

  • ySH109 = ySH103 9101(CATAA)-8440(TATAA)-his3 ( Saccharomyces cerevisiae )

  • ySH111 = ySH103 9000(TATAA)8440(TATAA)-his3 ( Saccharomyces cerevisiae )

  • ySH112 = ySH103 Sc3121-his3 via pSH222 ( Saccharomyces cerevisiae )

  • ySH113 = ySH103 Sc3621-his3 via pSH223 ( Saccharomyces cerevisiae )

  • ySH114 = ySH103 his3-161 (0T) via pSH224 ( Saccharomyces cerevisiae )

  • ySH115 = ySH103 his3-151 (C to A) via pSH225 ( Saccharomyces cerevisiae )

  • ySH117 = ySH103 Sc3619-his3 via pSH227 ( Saccharomyces cerevisiae )

  • ySH118 = ySH103 Sc2883-his3 via pSH228 ( Saccharomyces cerevisiae )

  • ySH12-161 KY320 a sua7::ADE2 tbp (pSH161) ( Saccharomyces cerevisiae )

    IIB-475.

  • ySH12-162 KY320 a sua7::ADE2 tbp (pSH162) ( Saccharomyces cerevisiae )

    IIB-475.

  • ySH120 = ySH103 Sc2884-his3 ( Saccharomyces cerevisiae )

  • ySH121 = ySH103 his3 p ( Saccharomyces cerevisiae )

  • ySH16-UT ( Saccharomyces cerevisiae )

    IIBact-245. KY320 a toa7::ADE2 tbp (TOA1/YEp195, TBP/YCp22).

  • ySH2/pRS316IIB ( Saccharomyces cerevisiae )

    IIB-262. KY320 sua7::ADE2 (pRS316yIIB).

  • ySH21-31 = ySH1 A5-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH22-31 = ySH1 Abfl-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH23-31 = ySH1 Ace1-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH24-31 = ySH1 Bas2-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH25-31 = ySH1 dAdT-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH26-31 = ySH1 Hap1-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH27-31 = ySH1 Hap2-5-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH28-31 = ySH1 Hsf-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH29-31 = ySH1 Gal4-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH3 ( Saccharomyces cerevisiae )

    S288C a ura3-52 lys2 202 trp1 161 his3 200 leu2 1 IIBts.

  • ySH30-31 = ySH1 Gcn4-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH31-31 = ySH1 L9-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH32-31 = ySH1 Ppr1-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH33-31 = ySH1 Put3-his3 (pSH1) ( Saccharomyces cerevisiae )

  • ySH34-31 = ySH1 Rap1-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH35-31 = ySH1 Reb1-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH36-31 = ySH1 Leu3-his3 (pSH31) ( Saccharomyces cerevisiae )

  • ySH4 ( Saccharomyces cerevisiae )

    S288C ura3-52 lys2 202 trp1 161 his3 200 leu2 1 IIBts.

  • ySH5 (FY7 his3:cyc1-1019-his3-TRP1) ( Saccharomyces cerevisiae )

  • ySH6-30 (ySH1 gcn4 1 (pSH30)) ( Saccharomyces cerevisiae )

    IIB-409.

  • ySH7 (KY320 a gcn4 1 HIS3+) ( Saccharomyces cerevisiae )

    IIB422.

  • ySH8-31 (ySH6 HIS3+ (pSH31)) ( Saccharomyces cerevisiae )

    IIB-422.

  • YTT199 = W1588C isw1::ADE2 isw2::LEU2 ( Saccharomyces cerevisiae )

    MATa his3-11,15 trp1-1 ura3-1 can1-100 RAD5 isw1::ADE2 isw2::LEU2.

  • YTT223 = W1588C isw1::ADE2 chd1::TRP1 ( Saccharomyces cerevisiae )

    MATa his3-11,15 leu2-3,112 ura3-1 can1-100 RAD5 isw1::ADE2 chd1::TRP1.

  • YTT227 = W1588C isw1::ADE2 isw2::LEU2 chd1::TRP1 ( Saccharomyces cerevisiae )

    MATa his3-11,15 ura3-1 can1-100 RAD5 isw1::ADE2 isw2::LEU2 chd1::TRP1.

  • Z24, pTL7, GAL1-454 (TRP+) ( Saccharomyces cerevisiae )

    Grow on -TRP medium.

  • Z280 (mat a his3-200 leu2-3,112 ura3-52 rpb3+12CA5+Leu2) ( Saccharomyces cerevisiae )

    IZ280 is the strain carrying HA-tagged rpb3. It was constructed in Rick Youngs lab and was reported to work in the chromatin IP experiments by Mark Ptashne and David Bentley. The strain was received from Nancy Hannet (hanet@wi.mit.edu). According to her, "rpb3 + 12CA5 + Leu2" means "12CA5 is inserted directly after the start codon and Leu2 is inserted 3' of rpb3").

  • Z422, pTL7, GAL1-454 (TRP+) ( Saccharomyces cerevisiae )

    Grow on -TRP medium.

  • Z425, pTL7, GAL1-454 (TRP+) ( Saccharomyces cerevisiae )

    Grow on -TRP medium.

  • Z428, pTL7, GAL1-454 (TRP+) ( Saccharomyces cerevisiae )

    Grow on -TRP medium.

  • ZGY005 ( Saccharomyces cerevisiae )

    Obtained from Bruce Stillman (Cold Spring Harbor).

  • ZGY005 Dot1-9myc ( Saccharomyces cerevisiae )

    Tagged with Nasmyth 9-myc.

  • ZGY005-8 ( Saccharomyces cerevisiae )

    Obtained from Bruce Stillman (Cold Spring Harbor).

  • ZM280 ( Saccharomyces cerevisiae )

    MAT his3 1 leu2 0 lys2 0 ura30 TFC6-3XFLAG.

  • ZM281 ( Saccharomyces cerevisiae )

    MAT his3 1 leu2 0 lys2 0 ura30 TFC6-3XHSV.

  • ZM283 ( Saccharomyces cerevisiae )

    MAT his3 1 leu2 0 lys2 0 ura30 TFC6-3XV5.

  • ZM284 ( Saccharomyces cerevisiae )

    MAT his3 1 leu2 0 lys2 0 ura30 TFC6-3XVSV-G.

  • ZMY117 18F (ZMY 117 his3::ADE2) ( Saccharomyces cerevisiae )

  • ZMY117 (isogenic to ZMY60 except for leu2::PET56) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56.

  • ZMY117 Spt shutoff ( Saccharomyces cerevisiae )

  • ZMY117 YCp33 ( Saccharomyces cerevisiae )

    Grow on -URA medium.

  • ZMY119 (TOA1 double shutoff in ZY117 background) ( Saccharomyces cerevisiae )

    URA.

  • ZMY127 (ZMY117 t’fd with YCplac33 -CEN/URA) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56. Maintain on -URA medium.

  • ZMY130 (TAF23 shutoff strain, ZMY117 background) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56.

  • ZMY131 ( Saccharomyces cerevisiae )

  • ZMY131 (117-269) TAF17 shutoff strain ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY141 (117-210) TBP knockout ( Saccharomyces cerevisiae )

    Maintain on -URA medium. CAUTION: Tends to revert. You may need to streak several colonies to plates + and - copper, and then use one that dies on copper.. URA.

  • ZMY141 (117-210) TBP knockout (duplicate stock) ( Saccharomyces cerevisiae )

    Maintain on -URA medium. CAUTION: Tends to revert. You may need to streak several colonies to plates + and - copper, and then use one that dies on copper.. URA.

  • ZMY141 (117-210) TBP shutoff strain ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY153 (117-193) TAF90 shutoff strain ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY154 (117-207) TFIIB shutoff strain ( Saccharomyces cerevisiae )

    Maintain on -URA medium. URAZMY117 background.

  • ZMY155 (117-203) TAF19 shutoff strain ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY59 (Ace-UBR1 only) ( Saccharomyces cerevisiae )

  • ZMY60 ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, LEU2, HIS3, ade2-101. KY114 background.

  • ZMY60 ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, LEU2, HIS3, ade2-101. KY114 background.

  • ZMY60 transformed with YCplac33 (CEN/URA) ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, LEU2, HIS3, ade2-101. Maintain on -URA medium.

  • ZMY66 TAF60 knockout, ZMY117 background ( Saccharomyces cerevisiae )

    Mat a, Ace-UBR1, Ace-ROX1, trp1-D1, ura3-52, HIS3, ade2-101, leu2::PET56.

  • ZMY66 (TAF60 shutoff strain, ZMY60 background) ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY69 (TAF130 shutoff strain, ZMY60 background) ( Saccharomyces cerevisiae )

    ZMY60 background.

  • ZMY71 (TFIIB shutoff strain, ZMY60 background) ( Saccharomyces cerevisiae )

    ZMY60 background.

  • ZMY75 (TAF130 shutoff, ZMY60 background) ( Saccharomyces cerevisiae )

    URA. Maintain on -URA medium.

  • ZMY87 (TAF17 shutoff strain, ZMY 60 background) ( Saccharomyces cerevisiae )

    ZMY60 background.

  • ZMY95 (TAF23 shutoff strain, ZMY60 background) ( Saccharomyces cerevisiae )

    ZMY60 background.

  • ZMY97 (TAF67 shutoff strain, ZMY60 background) ( Saccharomyces cerevisiae )

    ZMY60 background.

  • ZY71 ( Saccharomyces cerevisiae )

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Last updated: 2015-11-30T09:04:06.924-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016