The CytoGenomics Core provides an invaluable technical resource to the investigators of the BWH, MGH, and affiliated institutions. Services are also available to external academic and commercial laboratories; these should contact us via our website for sample submission and pricing. Cytogenetic studies can provide insight into regions of the genome that are pathogenetic in various neoplasms leading to an understanding of the molecular pathways participating in the biology of cancer. It is appropriate to consider cytogenetics as a fundamental adjunct to a variety of investigations underway, including basic and clinical research. For example, a rather simple cytogenetic analysis of mouse ES cells to determine ploidy prior to injections into blastulas leads to a greater success rate in establishing founders for knock-out and knock-in experiments. The primary chromosomal assignment of a gene by a FISH experiment may lead to correlation of a disease with that gene. Other cytogenetic studies may be important in establishing a diagnosis for correlation with clinical outcome. The advent of molecular probes for FISH analysis has facilitated cytogenetic studies in the mouse, and other model organisms and this Core aggressively implements such technologies.
In addition to state-of-the-art analyses for human samples, the BWH CytoGenomics Core also performs routine mouse karyotyping and a variety of other molecular cytogenetic analyses, services not easily obtainable elsewhere.
Microarray scanner with SureScan High Resolution Technology.
Array Comparative Genomic Hybridization oven for hybridization of test + reference onto array.
The X-15R spins up to three liters of sample at an industry-leading 5,250 x g, with a robust refrigeration system that cools from ambient to 4C in less than 4 minutes – over 10 minutes faster than other three liter centrifuges. Save even more time with optional ARIES rotor technology that accepts up to 50 grams (effectively one tube!) of rotor imbalance, allowing you to eye-balance your samples with confidence. The X-15R is the ideal choice for all your cellular separation applications. Max speed of 10,200 rpm (11,400 x g) and temperature control with 3 liters of volume process capability.
Max speeds up to 21,000 rpm (53,300 x g) and capable processing up-to 4 liters of volume. With temperature control
Sterile handling of tissue cultures with UV light sterilization capability.
Incubator/shaker for bacterial cultures.
Fluorescence microscope for multi-color FISH imaging.
Fluorescence microscope for two-color FISH imaging.
Fluorescence microscope for paraffin tissue sample FISH imaging.
Phase contrast microscope.
Low speed centrifuge used to separate and deposit a monolayer of cells on slides while maintaining cellular integrity.
Vacuum centrifugation to concentrate sample of interest. Core hosts two instances of this instrument.
Tissue culture incubator with percent carbon dioxide control.
"CGH assesses genomic imbalances in neoplasms, providing an entry point into the identification of genes that are amplified or deleted, which might play a role in the pathogenesis or pathobiology of a tumor. This method is based on hybridization of differentially-labeled tumor DNA with normal control DNA onto normal human metaphase chromosomes or array-based platforms."
"Standard metaphase spreads of human and mouse chromosomes are analyzed. The primary banding technique is GTG banding. However, other banding methods, such as fluorescent R-, QFQ-, CBG-, and NOR-banding can be used depending on the chromosomal region to be evaluated."
"We have extensive experience using FISH in both metaphase and interphase cells for human and mouse studies. Interphase cells from freshly disaggregated tissue as well as from paraffin-embedded tissues and tissue sections can also be analyzed. We employ a wide spectrum of FISH probes including painting, repetitive and locus-specific probes, and we have developed probes for specific applications when comparable probes are not commercially available."