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HSCI iPS Cell Core Facility

Directors: Cowan, Chad A., Ph.D.; Rossi, Derrick J., Ph.D.

Location: Harvard University, Bauer Building Room B01, 7 Divinity Avenue, Cambridge, MA 02138

Summary:

The HSCI iPS Core facility was created to accelerate research in the stem cell field by facilitating the derivation and distribution of iPS cell lines. Disease-specific iPS lines provide us with a unique opportunity to study the mechanisms of disease and ultimately to develop new treatments. The iPS Core serves as a repository for iPS cells produced by HSCI scientists and functions as a laboratory to produce disease-specific lines for sharing with the HSCI and broader research community.

Affiliations:

People:

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Protocols

  • Standard operating procedures for the culture of human iPS ( Protocol )

    In general, caring for hiPS cells is identical to caring for hESCs, which you are assumed to have experience with prior to requesting these cell lines. While there are different ways of culturing these cell lines, the general concepts are the same. Provided are the general instructions we have used to culture hiPS cells (primarily adapted from the protocols from WiCell).

Reagents

  • 11b - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

    Source Cells: Dermal Fibroblasts.

    Gender: Male.

  • 15b - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

    Source Cells: Dermal Fibroblasts.

    Gender: Female.

  • 17a - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

  • 18a - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

  • 18b - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

  • 18c - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

  • 20b - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Male. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4.

  • 27e-ALS (SOD1/G85R) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Genetic Disorder: Amyotrophic lateral sclerosis SOD1(G85R). Derivation Method: Viral Integration
    Retrovirus, Transgenes = Oct4, Sox2, KLF4.

  • 29d-ALS (SOD1/L144F) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4 Genetic Disorder: Amyotrophic lateral sclerosis (SOD1D90A).

  • 29e-ALS (SOD1/L144F) ( Induced pluripotent stem cell line )

    Source Cells: Dermal fibroblasts. Gender: Female. Derivation Method: Viral integration
    retrovirus transgenes = Oct4, Sox2, KLF4 Genetic Disorder: Amyotrophic lateral sclerosis (SOD1D90A).

  • BJ (ATCC CRL-2522) ( Primary fibroblast cell line )

    The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines.

    A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000.

    These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82.

    They are telomerase negative.

    Cells from ATCC ampules have the capacity to proliferate to a maximum of 72 population doublings before the onset of senescence.

  • BJ epi iPS-C - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Source of Cells: fibroblasts (purchased from ATCC)

  • BJ RiPS-A - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Source of Cells: fibroblasts (purchased from ATCC)

  • BJ RiPS-D - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Source of Cells: fibroblasts (purchased from ATCC)

  • BJ RiPS-E - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Source of Cells: fibroblasts (purchased from ATCC)

  • BJ SiPS -C - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    gender=male
    Healthy donor control
    Sendai Virus

  • BJ SiPS -D - Healthy Donor Control (Male) ( Induced pluripotent stem cell line )

    gender=male
    Healthy donor control
    Sendai Virus

  • ery ED3 SiPSA - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • ery ED4 SiPSA - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • HUES 01 ( Embryonic stem cell line )

    Cell Line: HUES 1
    NIH Registration Number: 0014
    NIH Approval Number: NIHhESC-09-0014
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 02 ( Embryonic stem cell line )

    Cell Line: HUES 2
    NIH Registration Number: 0015
    NIH Approval Number: NIHhESC-09-0015
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 03 HB9::GFP ( Embryonic stem cell line )

    Stably transfected HUES 3 cell lines expressing GFP under control of the Murine Hb9 promoter
    Marks spinal cord motor neurons

    Derived from
    HUES 3
    NIH Registration Number: 0016
    NIH Approval Number: NIHhESC-09-0016
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 04 ( Embryonic stem cell line )

    Cell Line: HUES 04
    NIH Registration Number: 0017
    NIH Approval Number: NIHhESC-09-0017
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 06 ( Embryonic stem cell line )

    Cell Line: HUES 6
    NIH Registration Number: 0019
    NIH Approval Number: NIHhESC-09-0019
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 07 ( Embryonic stem cell line )

    Cell Line: HUES 7
    NIH Registration Number: 0020
    NIH Approval Number: NIHhESC-09-0020
    Karyotype: Normal, 46XY
    Ethnicity: Europe (Closest Reference Population: French/Basque)
    Pluripotency Tested: In vitro, in vivo

  • HUES 08 ( Embryonic stem cell line )

    Cell Line: HUES 8
    NIH Registration Number: 0021
    NIH Approval Number: NIHhESC-09-0021
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 09 ( Embryonic stem cell line )

    Cell Line: HUES 9
    NIH Registration Number: 0022
    NIH Approval Number: NIHhESC-09-0022
    Karyotype: Abnormal, 46XX, Inv9
    Pluripotency Tested: In vitro, in vivo

  • HUES 10 ( Embryonic stem cell line )

    Cell Line: HUES 10
    NIH Registration Number: 0023
    NIH Approval Number: NIHhESC-09-0023
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 11 ( Embryonic stem cell line )

    Cell Line: HUES 11
    NIH Registration Number: 0024
    NIH Approval Number: NIHhESC-09-0024
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 12 ( Embryonic stem cell line )

    Cell Line: HUES 12
    NIH Registration Number: 0025
    NIH Approval Number: NIHhESC-09-0025
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 14 ( Embryonic stem cell line )

    Cell Line: HUES 14
    NIH Registration Number: 0027
    NIH Approval Number: NIHhESC-09-0027
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 15 ( Embryonic stem cell line )

    Cell Line: HUES 15
    NIH Registration Number: 0028
    NIH Approval Number: NIHhESC-09-0028
    Karyotype: Normal, 46XX
    Pluripotency Tested: In vitro, in vivo

  • HUES 16 ( Embryonic stem cell line )

    Cell Line: HUES 16
    NIH Registration Number: 0029
    NIH Approval Number: NIHhESC-09-0029
    Karyotype: Normal, 46XY
    Ethnicity: Europe (Closest Reference Population: French)
    Pluripotency Tested: In vitro, in vivo

  • HUES 17 ( Embryonic stem cell line )

    Cell Line: HUES 17
    NIH Registration Number: 0030
    NIH Approval Number: NIHhESC-09-0030
    Karyotype: Normal, 46XY
    Pluripotency Tested: In vitro, in vivo

  • HUES 19 ( Embryonic stem cell line )

    Cell Line: HUES 19
    NIH Registration Number: 0032
    NIH Approval Number: NIHhESC-09-0032
    Karyotype: 46XY

  • HUES 20 ( Embryonic stem cell line )

    Cell Line: HUES 20
    NIH Registration Number: 0033
    NIH Approval Number: NIHhESC-09-0033
    Karyotype: 46XY

  • HUES 21 ( Embryonic stem cell line )

    Cell Line: HUES 21
    NIH Registration Number: 0034
    NIH Approval Number: NIHhESC-09-0034
    Karyotype: 46XX

  • HUES 22 ( Embryonic stem cell line )

    Cell Line: HUES 22
    NIH Registration Number: 0035
    NIH Approval Number: NIHhESC-09-0035
    Karyotype: 46XY

  • HUES 24 ( Embryonic stem cell line )

    Cell Line: HUES 24
    NIH Registration Number: 0037
    NIH Approval Number: NIHhESC-09-0037
    Karyotype: Abnormal, 47XY

  • HUES 44 ( Embryonic stem cell line )

    Cell Line: HUES 44
    NIH Registration Number: 0158
    NIH Approval Number: NIHhESC-12-0158

  • HUES 45 ( Embryonic stem cell line )

    Cell Line: HUES 45
    NIH Registration Number: 0076
    NIH Approval Number: NIHhESC-10-0076
    Karyotype: Normal, 46XX

  • HUES 48 ( Embryonic stem cell line )

    Cell Line: HUES 48
    NIH Registration Number: 0053
    NIH Approval Number: NIHhESC-10-0053
    Karyotype: Normal, 46XX
    Derivation Method: Mechanical Dissection - Laser-mediated ICM

  • HUES 49 ( Embryonic stem cell line )

    Cell Line: HUES 49
    NIH Registration Number: 0054
    NIH Approval Number: NIHhESC-10-0054
    Karyotype: Normal, 46XX
    Derivation Method: Mechanical Dissection -
    Laser-mediated ICM isolation

  • HUES 53 ( Embryonic stem cell line )

    Cell Line: HUES 53
    NIH Registration Number: 0055
    NIH Approval Number: NIHhESC-10-0055
    Karyotype: Normal, 46XY
    Derivation Method: Mechanical Dissection - Laser-mediated ICM isolation

  • HUES 62 ( Embryonic stem cell line )

    Cell Line: HUES 62
    NIH Registration Number: 0065
    NIH Approval Number: NIHhESC-10-0065
    Karyotype: Normal, 46XX
    Derivation Method: Mechanical Dissection - Laser-mediated ICM isolation

  • HUES 64 ( Embryonic stem cell line )

    Cell Line: HUES 64
    NIH Registration Number: 0067
    NIH Approval Number: NIHhESC-10-0067
    Karyotype: Normal, 46XY
    Derivation Method: Mechanical Dissection - Laser-mediated ICM isolation

  • HUES 66 ( Embryonic stem cell line )

    Cell Line: HUES 66
    NIH Registration Number: 0057
    NIH Approval Number: NIHhESC-10-0057
    Pluripotency Tested: In Vitro (EBs)

  • STiPS A3 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • STiPS A4 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • STiPS A5 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • STiPS A6 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • STiPS O-XX1 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

  • STiPS O-XX2 - Healthy Donor Control (Female) ( Induced pluripotent stem cell line )

    Healthy Donor Control
    Female
    Sendai Virus

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Last updated: 2012-07-20T10:34:27.044-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016