The Harvard Center for Biological Imaging (HCBI) was created to foster collaborative research in the most state-of-the-art facility available. To do this, a unique partnership with Carl Zeiss Microscopy has been established in which microscopy systems are replaced every 2-3 years, ensuring access to only the most up-to-date imaging systems. The facility is open 24 hours/day, 7 days/week and access to the HCBI is open to all academic and industrial researchers.
Workstation 1: High-end PC for graphics work running Arivis, Imaris, Volocity, Fiji(ImageJ), and ZEN 2 lite. System can be used for 3D rendering, quantitation and analysis of data.
Workstation 2: High-end PC for processing work which includes up-to-date versions of ZEN Black and ZEN Blue with all modules enabled. The station is primarily used for processing 3DSIM and PALM/STORM data and conversion of AxioScan files to .tif/.bigtif format.
Workstation 3: High-end PC for graphics work running ZEN Black Lightsheet edition and Arivis 4D Vision. The computer features 30TB of data storage and is EXCLUSIVELY used for processing data sets acquired on the Lightsheet microscope.
Are you tired of waiting for your tissues to clear? Speed up the Process with the X-CLARITY Tissue Clearing System. Perform the lipid removal step of the CLARITY protocol in as little as3 hours (2mm slice) or 24 hours (Whole brain). HCBI provides all the consumables and buffers needed for this step in the protocol!
"The unique Multiview Light Sheet Fluorescence Microscope allows you to record the development of large, living samples and gently image them to deliver exceptionally high information content.
It is also fast. Lightsheet Z.1 gives you the tools you need to work at unprecedented speed and acquire images of your whole sample volume at sub-cellular resolution – in a fraction of the time it takes using other techniques."
Inverted LSM Pascal (from Schier Lab) running the old AIM software. Lasers: 405, Argon (458,488,514) and 543 HeNe.
Inverted LSM 510 META (from Melton Lab) running AIM software. Lasers: 405 Diode, 488 (458,477,514) Argon, 543 HeNe and 633 HeNe.
Upright system (from the Imaging Center) LSM 510 META running the AIM software. Lasers: Argon (458, 488, 514) 543 HeNe and 633 HeNe.
The LSM 880 confocal microscope offers 34-channels of supreme sensitivity, owing to its GaAsP detectors with the added bonus of a super-resolution Airyscanner. This extraordinary point scanner can simultaneously image and spectrally separate up to 10 different dyes, create high resolution 3D images over large tiled areas, or image single molecules through fluorescence correlation techniques. The unparalleled sensitivity of this system also makes it the ideal live-cell imaging point scanner, allowing for high-speed confocal acquisition with brilliant signal to noise ratios. The Airyscan detector removes the requirement for a tightly closed pinhole allowing more of the light collected from your sample to reach the detector. Along with deconvolution Airyscan provides a 1.7x improvement over the theoretical diffraction limit.
Laser lines: 405, 458, 488, 514, 561, 594, 633
Commonly imaged dyes: all visible spectrum and near IR dyes (405-700nm)
Service includes individual training on the instruments.
Open to all academic and commercial researchers on an hourly rate basis. See HCBI website for details.