This lab's mission is "to provide high standard cell sorting and analysis services to the research community at the Brigham and Women’s Hospital and the Longwood Medical area."
3 lasers: Blue 488nm; Red 640nm; UV 355nm
Cell sorting protocol.
Service includes: single cell sorting (cloning) into 96-well and 384-well plates, sorting (up to 10 colors) of multicolor immunophenotyped samples, sorting and analysis of GFP, YFP, mTomato, gene expression proteins, sorting stem cell side populations using Hoechst, and sorting up to 4 populations at once into Eppendorf, 5 ml or 15 ml tubes.
Uses standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
"Specialties: Multicolor immunophenotyping. 11 colors including Qdots by customer choice; DNA analysis with Hoechst 33342 and 33358; DAPI, PI, YoYo1, DRAQ5; Analysis of Side Population with Hoechst 33342 and cells surface staining; GFP, YFP, td-Tomato; High through put flow cytometry combined with Interllycyt system. Utilizing multiplex Luminex bead assays."